Current Trends in <strong>Biotechnology</strong> <strong>and</strong> <strong>Pharmacy</strong> Vol. 5 (3) 1346 -1352 July 2011, ISSN 0973-8916 (Print), 2230-7303 (Online) 1351 fertilizers had no effect on the yields <strong>of</strong> dry roots (14). Highest root yield <strong>of</strong> aswag<strong>and</strong>ha was obtained with the application <strong>of</strong> 20 kg N <strong>and</strong> 40 kg P 2 O 5 ha -1 (11). HPLC analysis revealed that application <strong>of</strong> 50% NPK + 10 tonnes <strong>of</strong> farm yard manure ha -1 gave maximum yield <strong>of</strong> forskolin. The statistical analysis revealed that fresh <strong>and</strong> dry weights <strong>of</strong> roots, number <strong>of</strong> roots plant -1 <strong>and</strong> root length were significant compared to controls <strong>and</strong> positively correlated with each other. It is suggested that appropriate dose <strong>of</strong> application <strong>of</strong> fertilizers at appropriate time will result in higher tuber yield <strong>and</strong> forskolin content. Our results also suggest that time <strong>of</strong> harvest for optimum yields <strong>of</strong> tuber is also important. References 1. Srinivasappa, K.N., Anuradha, M.N., Farooqi, A.A., Kathiresan, C., Suresh, H.C., <strong>and</strong> Shivan<strong>and</strong>a, T.N. (2007). Use <strong>of</strong> bi<strong>of</strong>ertilizers in cultivation <strong>of</strong> medicinal <strong>and</strong> aromatic plants - a review. BIOMED, 2: 235-256. 2. Paturde, J.K., Wankhade, S.G., Khode P.P, Chatol, P.U, Deo, D.D. <strong>and</strong> Bhuyar, S.A. (2002). Effect <strong>of</strong> organic manures <strong>and</strong> plant population on yield <strong>of</strong> safed musli (Chlorophytum borivallianum). Agricultural Science Digest, 22: 51-52. 3. Sivakumar, C.V., <strong>and</strong> Vidhyasekaran, P. (1990). Control <strong>of</strong> Meloidogyne incognita on Coleus Forskohlii with Paecilomyces lilacinus in farm yard manure amended <strong>and</strong> non-amended soil. Journal <strong>of</strong> Biological Control, 4: 68-69. 4. Veeraraghavathatham, D, Venkatachalam, R., <strong>and</strong> Sundararajan., S. (1988). Effect <strong>of</strong> various levels <strong>of</strong> N, P <strong>and</strong> K on the tuber Yield <strong>of</strong> Coleus forskohlii. South Indian Horticulture, 36: 252-257. 5. Geetha, K., <strong>and</strong> Madhavan Nair., K.P. (1993). Effect <strong>of</strong> N <strong>and</strong> K in Coleus. Journal <strong>of</strong> Tropical Agricultural, 31: 198-203. 6. Somanath, I., Bhaskar, S., <strong>and</strong> Sreenivasmurthy C.A. (2007). Influence <strong>of</strong> FYM <strong>and</strong> NPK on uptake <strong>of</strong> nutrients <strong>and</strong> growth <strong>of</strong> Coleus forskohlii. BIOMED, 2: 265-271. 7. Tiwari, J.P., Tiwari, A.B., Tiwari, G., <strong>and</strong> Singh A.K. (2000). Effect <strong>of</strong> nitrogen application on growth <strong>and</strong> yield <strong>of</strong> Acorus calamus. Proceedings <strong>of</strong> the National Seminar on the Research <strong>and</strong> Development in Aromatic Plants. Current trends in biology, uses, production <strong>and</strong> marketing <strong>of</strong> essential oils, Lucknow, India, 30-31 July 1999. Journal <strong>of</strong> Medicinal <strong>and</strong> Aromatic Plant Sciences, 22: 636-638. 8. Maryada, M, Kaushal, S.K <strong>and</strong> Kaistha, B.P. (2001). Response <strong>of</strong> aswag<strong>and</strong>ha (Withania somnifera) to nitrogen fertilization under mid hill conditions <strong>of</strong> Himachal Pradesh. Himachal Jourmal <strong>of</strong> Agriculture Research, 27: 77-80. 9. Hedge, D.M. (1988). Response <strong>of</strong> periwinkle (Catharanthus roseus L.) to nitrogen, phosphorus <strong>and</strong> potassium fertilization. Agricultural Research Journal <strong>of</strong> Kerala, 26: 227-233. 10. Maheshwari, S.K., Yadav, S, Gangrade, S.K <strong>and</strong> Trivedi K.C. (1988). Effect <strong>of</strong> fertilizers on growth, root <strong>and</strong> alkaloid yield <strong>of</strong> rauwolfia (Rauwolfia serpentina). Indian Journal <strong>of</strong> Agricultural Sciences, 58: 87- 488. 11. Maheshwari, S.K. <strong>and</strong> Yadav, S. (1981). Response <strong>of</strong> Withania somnifera to variable levels <strong>of</strong> N <strong>and</strong> P rainfed. Paper presented at the IV All India Workshop on Medicinal Nageswara Rao et al
Current Trends in <strong>Biotechnology</strong> <strong>and</strong> <strong>Pharmacy</strong> Vol. 5 (3) 1346 -1352 July 2011, ISSN 0973-8916 (Print), 2230-7303 (Online) 1352 <strong>and</strong> Aromatic Plants, Madhurai, 31 st August-September 3, pp: 11-12. 12. Sheu, B.W., Juan Y.H. <strong>and</strong> Yan H.F. (2000). The effects <strong>of</strong> organic <strong>and</strong> inorganic fertilizers on the growth <strong>and</strong> yield <strong>of</strong> Herng- Chuen Yams, Dioscorea doryophora. Bulletin <strong>of</strong> National Pingtung University <strong>of</strong> Science <strong>and</strong> Technology, 8: 103-107. 13. Muthumanickam, D <strong>and</strong> Balakrishnamurthy G. (1999). Studies on nutritional requirement for aswag<strong>and</strong>ha (Withania somnifera) in sheuroy hills <strong>of</strong> Tamilnadu, India. Journal <strong>of</strong> species <strong>and</strong> Aromatic Crops, 8: 179-183. 14. Nigam K.B., Rawat G.S. <strong>and</strong> Prasad B. (1984). Effect <strong>of</strong> methods <strong>of</strong> sowing, plant density <strong>and</strong> fertility level on aswag<strong>and</strong>ha (Withania somnifera Dunal.). South Indian Horticulture 32: 356-359. Effect <strong>of</strong> NPK <strong>and</strong> farm yard manure on the accumulation <strong>of</strong> forskolin
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