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full issue - Association of Biotechnology and Pharmacy

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Current Trends in <strong>Biotechnology</strong> <strong>and</strong> <strong>Pharmacy</strong><br />

Vol. 5 (3) 1346 -1352 July 2011, ISSN 0973-8916 (Print), 2230-7303 (Online)<br />

1351<br />

fertilizers had no effect on the yields <strong>of</strong> dry roots<br />

(14). Highest root yield <strong>of</strong> aswag<strong>and</strong>ha was<br />

obtained with the application <strong>of</strong> 20 kg N <strong>and</strong> 40<br />

kg P 2<br />

O 5<br />

ha -1 (11). HPLC analysis revealed that<br />

application <strong>of</strong> 50% NPK + 10 tonnes <strong>of</strong> farm<br />

yard manure ha -1 gave maximum yield <strong>of</strong><br />

forskolin. The statistical analysis revealed that<br />

fresh <strong>and</strong> dry weights <strong>of</strong> roots, number <strong>of</strong> roots<br />

plant -1 <strong>and</strong> root length were significant compared<br />

to controls <strong>and</strong> positively correlated with each<br />

other. It is suggested that appropriate dose <strong>of</strong><br />

application <strong>of</strong> fertilizers at appropriate time will<br />

result in higher tuber yield <strong>and</strong> forskolin content.<br />

Our results also suggest that time <strong>of</strong> harvest for<br />

optimum yields <strong>of</strong> tuber is also important.<br />

References<br />

1. Srinivasappa, K.N., Anuradha, M.N.,<br />

Farooqi, A.A., Kathiresan, C., Suresh, H.C.,<br />

<strong>and</strong> Shivan<strong>and</strong>a, T.N. (2007). Use <strong>of</strong> bi<strong>of</strong>ertilizers<br />

in cultivation <strong>of</strong> medicinal <strong>and</strong><br />

aromatic plants - a review. BIOMED, 2:<br />

235-256.<br />

2. Paturde, J.K., Wankhade, S.G., Khode P.P,<br />

Chatol, P.U, Deo, D.D. <strong>and</strong> Bhuyar, S.A.<br />

(2002). Effect <strong>of</strong> organic manures <strong>and</strong> plant<br />

population on yield <strong>of</strong> safed musli<br />

(Chlorophytum borivallianum).<br />

Agricultural Science Digest, 22: 51-52.<br />

3. Sivakumar, C.V., <strong>and</strong> Vidhyasekaran, P.<br />

(1990). Control <strong>of</strong> Meloidogyne incognita<br />

on Coleus Forskohlii with Paecilomyces<br />

lilacinus in farm yard manure amended <strong>and</strong><br />

non-amended soil. Journal <strong>of</strong> Biological<br />

Control, 4: 68-69.<br />

4. Veeraraghavathatham, D, Venkatachalam,<br />

R., <strong>and</strong> Sundararajan., S. (1988). Effect <strong>of</strong><br />

various levels <strong>of</strong> N, P <strong>and</strong> K on the tuber<br />

Yield <strong>of</strong> Coleus forskohlii. South Indian<br />

Horticulture, 36: 252-257.<br />

5. Geetha, K., <strong>and</strong> Madhavan Nair., K.P.<br />

(1993). Effect <strong>of</strong> N <strong>and</strong> K in Coleus. Journal<br />

<strong>of</strong> Tropical Agricultural, 31: 198-203.<br />

6. Somanath, I., Bhaskar, S., <strong>and</strong><br />

Sreenivasmurthy C.A. (2007). Influence <strong>of</strong><br />

FYM <strong>and</strong> NPK on uptake <strong>of</strong> nutrients <strong>and</strong><br />

growth <strong>of</strong> Coleus forskohlii. BIOMED, 2:<br />

265-271.<br />

7. Tiwari, J.P., Tiwari, A.B., Tiwari, G., <strong>and</strong><br />

Singh A.K. (2000). Effect <strong>of</strong> nitrogen<br />

application on growth <strong>and</strong> yield <strong>of</strong> Acorus<br />

calamus. Proceedings <strong>of</strong> the National<br />

Seminar on the Research <strong>and</strong> Development<br />

in Aromatic Plants. Current trends in<br />

biology, uses, production <strong>and</strong> marketing <strong>of</strong><br />

essential oils, Lucknow, India, 30-31 July<br />

1999. Journal <strong>of</strong> Medicinal <strong>and</strong> Aromatic<br />

Plant Sciences, 22: 636-638.<br />

8. Maryada, M, Kaushal, S.K <strong>and</strong> Kaistha,<br />

B.P. (2001). Response <strong>of</strong> aswag<strong>and</strong>ha<br />

(Withania somnifera) to nitrogen<br />

fertilization under mid hill conditions <strong>of</strong><br />

Himachal Pradesh. Himachal Jourmal <strong>of</strong><br />

Agriculture Research, 27: 77-80.<br />

9. Hedge, D.M. (1988). Response <strong>of</strong><br />

periwinkle (Catharanthus roseus L.) to<br />

nitrogen, phosphorus <strong>and</strong> potassium<br />

fertilization. Agricultural Research Journal<br />

<strong>of</strong> Kerala, 26: 227-233.<br />

10. Maheshwari, S.K., Yadav, S, Gangrade, S.K<br />

<strong>and</strong> Trivedi K.C. (1988). Effect <strong>of</strong> fertilizers<br />

on growth, root <strong>and</strong> alkaloid yield <strong>of</strong><br />

rauwolfia (Rauwolfia serpentina). Indian<br />

Journal <strong>of</strong> Agricultural Sciences, 58: 87-<br />

488.<br />

11. Maheshwari, S.K. <strong>and</strong> Yadav, S. (1981).<br />

Response <strong>of</strong> Withania somnifera to variable<br />

levels <strong>of</strong> N <strong>and</strong> P rainfed. Paper presented<br />

at the IV All India Workshop on Medicinal<br />

Nageswara Rao et al

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