full issue - Association of Biotechnology and Pharmacy

Current Trends in Biotechnology and Pharmacy
Vol. 5 (3) 1346 -1352 July 2011, ISSN 0973-8916 (Print), 2230-7303 (Online)
1348
forskolin was extracted in a soxhlett apparatus
with methanol and concentrated. TLC solvent
system (Rf 0.45, 20% EtOAc + petroleum ether)
showed the presence of forskolin. The extracts
were subjected to HPLC analysis.
Results
Fresh weight of the root : The crop was
harvested at 30, 90 and 150 days after
transplantation. Light irrigation was given one
day before harvesting for easy lifting up of roots.
Harvesting was carried out manually by digging
and uprooting the individual plants. The roots
were separated and washed to remove the soil.
Forskolin content was determined in roots 150
days after transplantation. The influence of
different fertilizer amendments on fresh weight
of the root [kg plant -1 ] at 30, 90 and 150 DAP of
growth in Coleus forskohlii is shown in table 1.
Maximum increase in fresh weight of roots was
recorded in treatment number 3 in all the stages
(at 30 DAP 0.135 kg plant -1 , at 90 DAP 0.347 kg
plant -1 at 150 DAP 0.600 kg plant -1 ) of growth.
Dry weight of the root and content of forskolin
: Influence of various fertilizer amendments on
dry weight of the root [kg plant -1 ] at 30, 90 and
150 DAP of growth in Coleus forskohlii is shown
in Table 2. In treatment number 3, maximum
fresh weight was observed i.e., at 30 DAP 9.1 g
plant -1 , at 90 DAP 18.9 g plant -1 at 150 DAP 60.0
g plant -1 . The fresh and dry weights of roots were
measured under different nitrogen regimes and
are shown in Table 3. The treatment 50% NPK +
10 tonnes of farm yard manure gave the best dry
root weight among the different treatments (Table
3). Samples were collected under different
treatments for quantification of forskolin.
Forskolin was extracted in a soxhlett apparatus,
concentrated and used for HPLC analysis (Fig.
1). HPLC analysis revealed that application of
50% NPK + 10 tonnes of farm yard manure ha -1
gave maximum yields of forskolin after 150 days
of harvest.
Discussion
Somanath et al. (6), in their study on
influence of FYM and NPK on uptake of
nutrients and growth of Coleus forskohlii
observed maximum increase in fresh weight of
roots in treatment number 3 (at 30 DAP 0.135
kg plant -1 , at 90 DAP 0.347 kg plant -1 at 150 DAP
0.600 kg plant -1 ). Application of N at 100 kg
ha -1 (half basal + half one month after
transplanting) along with a basal application of
Table 1. Effect of various fertilizer amendments on fresh weight of root (g per plant) at different
stages of growth in Coleus forskohlii
Treatments Days after planting
T
1
T
2
T
3
T
4
T
5
T
6
30 90 150
: Control – No manures 58 120 267
: 100% NPK recommended dose (N- 40 kg ha -1 ) 83 340 490
: 50% NPK + 10 tonnes of farm yard manure/ha 149 468 600
: 50% NPK + 5 tonnes of vermicompost/ha 100 375 510
: 20 tonnes of farm yard manure/ha 69 160 310
: Vermicompost 10 tonnes/ha 73 190 385
Effect of NPK and farm yard manure on the accumulation of forskolin