full issue - Association of Biotechnology and Pharmacy
full issue - Association of Biotechnology and Pharmacy
full issue - Association of Biotechnology and Pharmacy
You also want an ePaper? Increase the reach of your titles
YUMPU automatically turns print PDFs into web optimized ePapers that Google loves.
Current Trends in <strong>Biotechnology</strong> <strong>and</strong> <strong>Pharmacy</strong><br />
Vol. 5 (3) 1346 -1352 July 2011, ISSN 0973-8916 (Print), 2230-7303 (Online)<br />
1348<br />
forskolin was extracted in a soxhlett apparatus<br />
with methanol <strong>and</strong> concentrated. TLC solvent<br />
system (Rf 0.45, 20% EtOAc + petroleum ether)<br />
showed the presence <strong>of</strong> forskolin. The extracts<br />
were subjected to HPLC analysis.<br />
Results<br />
Fresh weight <strong>of</strong> the root : The crop was<br />
harvested at 30, 90 <strong>and</strong> 150 days after<br />
transplantation. Light irrigation was given one<br />
day before harvesting for easy lifting up <strong>of</strong> roots.<br />
Harvesting was carried out manually by digging<br />
<strong>and</strong> uprooting the individual plants. The roots<br />
were separated <strong>and</strong> washed to remove the soil.<br />
Forskolin content was determined in roots 150<br />
days after transplantation. The influence <strong>of</strong><br />
different fertilizer amendments on fresh weight<br />
<strong>of</strong> the root [kg plant -1 ] at 30, 90 <strong>and</strong> 150 DAP <strong>of</strong><br />
growth in Coleus forskohlii is shown in table 1.<br />
Maximum increase in fresh weight <strong>of</strong> roots was<br />
recorded in treatment number 3 in all the stages<br />
(at 30 DAP 0.135 kg plant -1 , at 90 DAP 0.347 kg<br />
plant -1 at 150 DAP 0.600 kg plant -1 ) <strong>of</strong> growth.<br />
Dry weight <strong>of</strong> the root <strong>and</strong> content <strong>of</strong> forskolin<br />
: Influence <strong>of</strong> various fertilizer amendments on<br />
dry weight <strong>of</strong> the root [kg plant -1 ] at 30, 90 <strong>and</strong><br />
150 DAP <strong>of</strong> growth in Coleus forskohlii is shown<br />
in Table 2. In treatment number 3, maximum<br />
fresh weight was observed i.e., at 30 DAP 9.1 g<br />
plant -1 , at 90 DAP 18.9 g plant -1 at 150 DAP 60.0<br />
g plant -1 . The fresh <strong>and</strong> dry weights <strong>of</strong> roots were<br />
measured under different nitrogen regimes <strong>and</strong><br />
are shown in Table 3. The treatment 50% NPK +<br />
10 tonnes <strong>of</strong> farm yard manure gave the best dry<br />
root weight among the different treatments (Table<br />
3). Samples were collected under different<br />
treatments for quantification <strong>of</strong> forskolin.<br />
Forskolin was extracted in a soxhlett apparatus,<br />
concentrated <strong>and</strong> used for HPLC analysis (Fig.<br />
1). HPLC analysis revealed that application <strong>of</strong><br />
50% NPK + 10 tonnes <strong>of</strong> farm yard manure ha -1<br />
gave maximum yields <strong>of</strong> forskolin after 150 days<br />
<strong>of</strong> harvest.<br />
Discussion<br />
Somanath et al. (6), in their study on<br />
influence <strong>of</strong> FYM <strong>and</strong> NPK on uptake <strong>of</strong><br />
nutrients <strong>and</strong> growth <strong>of</strong> Coleus forskohlii<br />
observed maximum increase in fresh weight <strong>of</strong><br />
roots in treatment number 3 (at 30 DAP 0.135<br />
kg plant -1 , at 90 DAP 0.347 kg plant -1 at 150 DAP<br />
0.600 kg plant -1 ). Application <strong>of</strong> N at 100 kg<br />
ha -1 (half basal + half one month after<br />
transplanting) along with a basal application <strong>of</strong><br />
Table 1. Effect <strong>of</strong> various fertilizer amendments on fresh weight <strong>of</strong> root (g per plant) at different<br />
stages <strong>of</strong> growth in Coleus forskohlii<br />
Treatments Days after planting<br />
T<br />
1<br />
T<br />
2<br />
T<br />
3<br />
T<br />
4<br />
T<br />
5<br />
T<br />
6<br />
30 90 150<br />
: Control – No manures 58 120 267<br />
: 100% NPK recommended dose (N- 40 kg ha -1 ) 83 340 490<br />
: 50% NPK + 10 tonnes <strong>of</strong> farm yard manure/ha 149 468 600<br />
: 50% NPK + 5 tonnes <strong>of</strong> vermicompost/ha 100 375 510<br />
: 20 tonnes <strong>of</strong> farm yard manure/ha 69 160 310<br />
: Vermicompost 10 tonnes/ha 73 190 385<br />
Effect <strong>of</strong> NPK <strong>and</strong> farm yard manure on the accumulation <strong>of</strong> forskolin