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Current Trends in Biotechnology and Pharmacy Vol. 5 (3) 1338 -1345 July 2011, ISSN 0973-8916 (Print), 2230-7303 (Online) 1341 directly collected from the mucosal compartment. Biochemical analysis Estimation of protein: To 0.1 ml of homogenized jejunal sac, 0.9 ml of water and 4.5 ml of alkaline copper reagent were added and kept at room temperature for 10 minutes, then 0.5 ml of Folin’s reagent was added and the color developed was read after 20 minutes at 640 nm (14). The level of protein was expressed as mg/5 cm of jejunal segment. Estimation of glucose: Glucose was estimated in the serosal fluid of the everted intestinal sac by the glucose oxidase/peroxidase (GOD/POD) method (15) by the AGAPPE reagent kit, India. Glucose is oxidized by glucose oxidase into gluconic acid and hydrogen peroxide. Hydrogen peroxide in presence of peroxidase (POD) oxidizes the chromogen 4-aminoantipyrine compound to a red colored compound. The intensity of the red colored compound is proportional to the glucose concentration and is measured at 505 nm. The final color is stable for 2 hours. Estimation of Pyruvate: Pyurvate levels was estimated in the serosal fluid of the everted intestinal sac by DNPH method (16) based on reaction of pyruvate with DNPH which produces a color and the color was read at 520 nm. Estimation of lactate dehydrogenase: Lactate dehydrogenase was estimated in the serosal fluid of the everted intestinal sac by using UV kinetic method by commercially available AUTOPAK kit, India. Lactate dehydrogenase catalyzes the conversion of pyruvate to lactate and NADH to NAD. Lactate dehydrogenase activity in sample is directly proportional to the rate of decrease in the absorbance of NADH at 340 nm. Statistical Analysis: Data was analyzed by one way analysis of variance (ANOVA) followed by Tukey’s multiple comparison. The significant level was fixed at p
Current Trends in Biotechnology and Pharmacy Vol. 5 (3) 1338 -1345 July 2011, ISSN 0973-8916 (Print), 2230-7303 (Online) 1342 Body weight after fasting Fig. 4. Body weight of the control, 4 days and 6 days fasting rats. Results were shown as mean ± SD. P
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Page 175: (December 7-9, 2011) Venue: Karunya
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