full issue - Association of Biotechnology and Pharmacy

Current Trends in Biotechnology and Pharmacy
Vol. 5 (3) 1325 -1337 July 2011, ISSN 0973-8916 (Print), 2230-7303 (Online)
1336
different concentrations were screened, and were
found to increase the production of cephamycin
C as compared to control (Fig. 6).
The effect of inducer on cephamycin C
production: Diamines are known to increase the
production of cephamycin C (8,9). 1,3-
Diaminopropane (a three carbon diamine) was
used as an inducer for the production of
cephamycin C. It was added at 0.4-1.6% v/v to
the RSM optimized media supplemented with
amino acids. Fig. 7 shows the effect of 1,3-
diaminopropane on production of cephamycin
C. The optimum production of cephamycin C
obtained was 3973.20 ± 57.96 mg/l at 1.2% v/v
of 1,3-diaminopropane. 1,3-Diaminopropane
increases the enzyme levels of lysine-6-
aminotransferase and P6C dehydrogenase (the
first and second enzymes of the cephamycin
biosynthetic pathway) responsible for the
conversion of lysine to á-aminoadipic acid (a rate
limiting step) at transcriptional level (9).
Conclusion
Glycerol and yeast extract were found
to be suitable carbon and nitrogen source for the
production of cephamycin C from N.
lactamdurans NRRL 3802. Taguchi L 16
-
orthogonal array design demonstrated the effect
of Na 2
S 2
O 3
, yeast extract, K 2
HPO 4
and L-
glutamic acid in the production medium to be
significant. Further optimization of the medium
using RSM showed complex interaction between
them and also increased the production of
cephamycin C to 3042.19 ± 62.36 mg/l. There
was a 12.52 fold increase in production of
cephamycin C after RSM and 16.35 fold increase
after addition of metabolic precursors and
inducer.
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Lalit D. Kagliwal et al