April Journal-2009.p65 - Association of Biotechnology and Pharmacy

Current Trends in Biotechnology and Pharmacy
Vol. 3 (2) 155-161, April 2009. ISSN 0973-8916
explained by the susceptibility of the tested C.
albicans pathogen to similar bioactive compounds
produced by the Streptomyces isolates were
recovered from the same environment and to their
active broths that were extracted under the same
conditions and analyzed using the same kind of
UV spectrophotometer. Msameh (9) in his
investigation showed 12 out of 26 UV spectra
(46%) that are similar to each other, Saadoun and
Al-Momani (17) showed 12 UV spectra (46%)
that are similar to what was reported by Msameh
(9).
Although the UV-spectra is one of the
basic evidences to identify an antibiotic, similarities
in the UV spectra might explain that compounds
produced by strains from various areas have
similar structure. Similarities in the general UV
spectra and maximum absorbance peaks
presented in this investigation could explain the
ability of the Streptomyces sp. to produce
antibiotics. Betina (5) reported that several
antibiotics can be produced by the same microbial
species. For example, S. hygroscopicus, S.
griseus, S. lavendulae, S. albus and S.
aureofaciens produced a total of 58, 48, 39, 31
and 21 different antibiotics respectively (5). Some
secondary metabolites are produced as a group
of closely related structures; one strain of
Streptomyces produces 32 different
anthracyclines. Therefore, further investigation
is encouraged on the HPLC profile of the
Streptomyces extracts and HPLC-MS by
comparing with known standards. Some
antibiotics as cyclohexamide and actidione have
similar UV-spectra (9) and these spectra were
similar to the metabolites produced by S.
violaceusniger (16) and by Streptomyces isolate
A1 (17).
Although the active Streptomyces strains
reflect different ecological habitats, culture, and
UV analysis conditions, screened isolates showed
similar UV-spectra. Similarities in the general UV
spectra and/or absorbance peaks could explain
160
the ability of the various tested and reported
Streptomyces strains to produce similar active
compounds.
Acknowledgements
Appreciation is extended for Jordan
University of Science and Technology for the
administrative support.
References
1. Aghighi, S., Shahidi, G.H., Rawashdeh, R.,
Bataineh, S. and Saadoun, I. (2004). First
report of antifungal spectra of activity of
Iranian actinomycetes strains against
Alternaria solani, Alternaria alternate,
Fusarium solani, Phytophthora
megasperma, Verticillium dahliae and
Saccharomyces cervisiae. Asian Journal
of Plant Sciences 3, 463-471.
2. Barakate, M., Ouhdouch, Y., Oufdou, K.H.,
and Beaulieu, C. (2002). Characterization
of rhizospheric soil Strteptomycetes from
Moracon habitats and their antimicrobial
activities. World Journal of Microbiology and
Biotechnology 18, 49-54.
3. Blatz, R. H. (1998). Genetic manipulation
of antibiotic producing Streptomyces. Trends
in Microbiology 6, 76-83.
4. Berdy, J. (1995). Are actinomycetes
exhausted as a source of secondary
metabolites Proceeding of the nine
symposiums on the actinomycetes, pp. 13-34.
5. Betina, V. (1983). The chemistry and
biology of antibiotics. Elsevier Scientific
Publishing Company, The Netherlands, pp
35-58
6. Hacene, H., Sabaou, N., Bounage, N. and
Lefevre, G. (1994). Screening for nonpolyenic
antifungal antibiotics produced by
rare actinomycetales. Microbios 79, 81-85.
7. Iliæ, S.B., Konstantinoviæ, S.S., Todoroviæ,
Z.B. (2005). UV/VIS analysis and
Saadoun et al