April Journal-2009.p65 - Association of Biotechnology and Pharmacy

Current Trends in Biotechnology and Pharmacy
Vol. 3 (2) 155-161, April 2009. ISSN 0973-8916
Streptomyces. Several investigators determined
the UV spectra for the active fermented broths
of different Streptomyces isolates on the basis
that future studies may answer the question
whether the metabolites produced by these
isolates responsible for these absorbance peaks
or not (6, 7, 8, 16, 17). Furthermore, the use of
spectroscopy to distinguish polyenic from
nonpolyenic substances were used by several
authors (2, 6, 10). When Sahin and Ugur (22)
investigated the antimicrobial activity of some
Streptomyces isolates that were obtained from
the soils of Mugla province/Turkey, they observed
that MU123 isolate exhibited antifungal activity
against C. albicans (ATCC 10239) and C.
tropicalis (RSKK 665) with an inhibition zone
diameter of 11-20 mm and > 31 mm, respectively.
This isolate showed a UV spectrum (Fig. 1b)
similar to what was reported for the isolate 23 7
from Jordan (16) (Fig. 1a) with 2 maximum
absorbance peaks at 226 and 260 nm (Table 1).
The isolate 23 7
exhibited an inhibition zone
diameter of 16-20 mm against C. albicans (17)
and 25-30 mm against Trichoderma harasmii
and Aspergillus flavus (18). The two maximum
absorbance peaks by 23 7
isolate were at 223 and
250 nm (Table 1). This difference in the maximum
absorbance peaks might be explained by
spectroscopic instrument used in the analysis,
culture and extraction conditions. To confirm that
similarity, the UV-spectrum of the extracted broth
for the 23 7
isolate was repeated by using Jenway/
UK UV-visible spectrophotometer (Table 1, Fig.
1c). Data revealed a UV-spectrum similar to what
was reported for the isolate 23 7
(17) (Fig. 1a)
with 2 maximum absorbance peaks at 220 and
260 nm (Table 1). By using Unicam UVspectrophotometer,
the isolate (C5P1-6) of this
study was analyzed and a similar spectrum
exhibited with 2 maximum absorbance peaks at
225-250 and 300 nm (Table 1, Fig. 1d).
Saadoun et al. (16) in their analysis of
the UV-spectrum (200-500) of n-butanol extract
158
of yeast dextrose broth culture of Streptomyces
violaceusniger showed 2 maximum absorbance
peaks at 231 and 258 nm (Table 1, Fig. 2b). This
Streptomyces sp. was isolated from a sediment
sample, collected from a stream, Auburn,
Alabama-USA (13). S. violaceusniger exhibited
a 10 mm inhibition zone diameter against C.
albicans (16). In another study, Saadoun and Al-
Momani (17) determined the UV-spectra of the
fermented broth for the most active Streptomyces
isolates against C. albicans. They showed
absorbance peaks ranging between 230 and 300
nm and with the isolate A1 exhibiting similar UVspectra
and 2 absorbance peaks at 236 and 262
nm (Table 1, Fig. 2a). A1 was among the isolates
that was recovered from soils of North Jordan
with an activity of 35-40 mm against C. albicans
(17). Similarly, Lemriss and his colleagues (8)
when screened for nonpolyenic antifungal
metabolites in clinical isolates of actinomycetes
found that Streptomyces sp. 96.0333 exhibiting
antifungal activity of >30 mm against all the tested
fungi (Table 1) and a UV spectrum (Fig. 2c) as
the one reported by Saadoun and Al-Momani (17)
and Saadoun et al. (16) with 2 absorbance peaks
at 220-225 and 262 nm (Table 1). To confirm that,
the UV-spectrum of the extracted broth for the
A1 isolate was repeated by using Jenway/UK
UV-visible spectrophotometer (Table 1, Fig. 2d).
Data revealed a UV-spectrum similar to what
was reported for the isolate A1 by Saadoun and
Al-Momani (17) (Fig. 2a) and Saadoun et al. (16)
with 2 absorbance peaks at 240 and 280 nm (Table
1). Also isolates from this study (C1P2-6) and
from Iran (Ir 102) were compared under the same
cultural, extraction and UV analysis conditions.
Data indicated a similar UV spectrum with 2
absorbance peaks for both isolates at 200-225
and 275-300 nm (Table 1, Fig 3a and Fig 3b,
respectively). The isolate Ir 102 exhibited > 20
mm against Alternaria solani and A. alternate
(1). The wide peaks exhibited by the Ir 102 and
C5P1-6 isolates may be explained by the
Saadoun et al