April Journal-2009.p65 - Association of Biotechnology and Pharmacy

Current Trends in Biotechnology and Pharmacy
Vol. 3 (2) 155-161, April 2009. ISSN 0973-8916
extracts of clinical isolates of actinomycetes with
indication of similarities in the maximum
absorbance peaks (8). Similarly, a study by Iliæ et
al. (7) on 20 different Streptomyces isolates from
the soils of Southeastern Serbia indicated that the
UV spectra of the culture extracts for the active
isolates showed absorbance peaks ranging
between 221 and 240 nm. The UV spectra of the
active compounds in methanol showed peaks at
217 and 221 nm.
In this investigation we are comparing
the UV-spectra of some extracted culture broths
of active Streptomyces isolates with each other
as well as with other reported isolates obtained
156
from different habitats and analyzed under
different extraction and instrumental conditions.
Materials and Methods
Isolation and characterization of the active
strains
The procedures were performed as
described by Saadoun and Al-Momani (12) and
Saadoun et al. (21).
Antibacterial and antifungal agents
The activity of the Streptomyces isolates
towards bacteria and Candida albicans was
performed as described by Saadoun et al. (16)
and Saadoun and Al-Momani (17), respectively.
Table 1: Comparison of the UV-spectra of different Streptomyces isolates recovered from different habitats and
analyzed by different UV spectroscopic instruments.
a
ID: Inhibition zone diameter in mm as determined by agar disc diffusion method. b C: Candida; T: Trichoderma;
Tr : Trichophyton rubrum ; Al: Alternaria; As : Aspergillus c ND: Not determined
Saadoun et al