April Journal-2009.p65 - Association of Biotechnology and Pharmacy
April Journal-2009.p65 - Association of Biotechnology and Pharmacy
April Journal-2009.p65 - Association of Biotechnology and Pharmacy
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Current Trends in <strong>Biotechnology</strong> <strong>and</strong> <strong>Pharmacy</strong><br />
Vol. 3 (2) 155-161, <strong>April</strong> 2009. ISSN 0973-8916<br />
Comparative UV-spectra <strong>of</strong> fermented cultural extract <strong>of</strong><br />
antifungal-active Streptomyces isolates recovered from different<br />
ecological habitats<br />
1<br />
Ismail Saadoun, 1 Fouad AL-Momani, 1 Qotaiba Ababneh <strong>and</strong> 2 Shahidi Bonjar<br />
1<br />
Dept. <strong>of</strong> Biological Sciences, Jordan Univ. <strong>of</strong> Science <strong>and</strong> Technology, Irbid-22110, Jordan<br />
2<br />
Department <strong>of</strong> Plant Pathology, College <strong>of</strong> Agricultural Sciences, Bahonar University <strong>of</strong> Kerman, Kerman-Iran<br />
* For Correspondence : isaadoun@just.edu.jo<br />
Abstract<br />
UV-spectra <strong>of</strong> antifungal-active<br />
Streptomyces isolates were compared to<br />
previously reported spectra <strong>and</strong> analyzed under<br />
different extraction conditions. MU123<br />
Streptomyces isolate from Turkey exhibited a UV<br />
spectrum similar to 23 7<br />
isolate from Jordan with 2<br />
maximum absorbance peaks at (226 <strong>and</strong> 260 nm)<br />
<strong>and</strong> at (220 <strong>and</strong> 260 nm), respectively. This<br />
spectrum was repeated by the C5P1-6 isolate from<br />
Jordan with 2 maximum absorbance peaks at 225-<br />
250 <strong>and</strong> 300 nm. The aquatic species identified<br />
as S. violaceusniger showed 2 maximum<br />
absorbance peaks at 231 <strong>and</strong> 258 nm similar to<br />
the UV spectrum <strong>of</strong> a clinical isolate <strong>of</strong><br />
actinomycetes (Streptomyces sp. 96.0333) that<br />
exhibited 2 absorbance peaks at 220-225 <strong>and</strong> 262<br />
nm. When C1P2-6 isolate from Jordan was<br />
compared to Ir 102 from Iran under same cultural,<br />
extraction <strong>and</strong> UV analysis conditions, data<br />
revealed similar UV spectra with 2 absorbance<br />
peaks for both isolates at 200-225 <strong>and</strong> 275-300<br />
nm. Approximately 50% <strong>of</strong> reported active<br />
screened isolates exhibit similar UV-spectra which<br />
might reflect their habitats, culture <strong>and</strong> UV<br />
analysis conditions. Comparison <strong>of</strong> UV-spectra<br />
<strong>and</strong> absorption peaks <strong>of</strong> known antibiotics to that<br />
<strong>of</strong> active Streptomyces isolates might explain the<br />
ability <strong>of</strong> the same Streptomyces sp. to produce<br />
several antibiotics.<br />
Introduction<br />
Members <strong>of</strong> the order Actinomycetales<br />
are the most abundant soil microorganisms under<br />
a wide variety <strong>of</strong> conditions. They include many<br />
species that are characterized by the production<br />
<strong>of</strong> extracellular important bioactive compounds.<br />
Majority <strong>of</strong> those strains belong to species within<br />
the genus Streptomyces which produce two–<br />
thirds <strong>of</strong> the naturally occurring antibiotics world<br />
wide. Such strains were advocated as promising<br />
agents against several pathogens <strong>and</strong> are well<br />
known for their potential to produce a large<br />
number <strong>of</strong> inhibitory metabolites used in industry,<br />
pharmacy, including: antihelminthic, antitumor,<br />
antifungal agents (3, 4, 23). Several studies on<br />
the isolation, characterization <strong>and</strong> genotyping <strong>of</strong><br />
soil streptomycetes <strong>of</strong> Jordan have already been<br />
conducted (11, 13, 14, 15). Other studies showed<br />
the ability <strong>of</strong> different streptomycetes isolates to<br />
inhibit the growth <strong>of</strong> several multi-resistant Grampositive<br />
<strong>and</strong> Gram-negative pathogens (18, 19,<br />
20). Experiments on the nature <strong>of</strong> the inhibitory<br />
metabolite produced by S. violaceusniger<br />
showed a maximum absorption in the UV region<br />
at 210-260 nm (16). In another study,<br />
Streptomyces isolates active against C<strong>and</strong>ida<br />
albicans determined with UV-spectra<br />
absorbance peaks were either at 230 nm or 300<br />
nm or in between (17). Some <strong>of</strong> these spectra<br />
were similar to the UV-spectra <strong>of</strong> the active<br />
Comparison <strong>of</strong> UV spectrum analysis <strong>of</strong> active streptomycetes