LABKIT – CLINICAL DIAGNOSTIC REAGENT - Agentúra Harmony vos

HITACHI 704
INSTRUCCIONES DE TRABAJO
DIRECTIONS FOR USE
AUTOMATIZACIÓN
AUTOMATION
LABKIT – CLINICAL DIAGNOSTIC REAGENT
CHEMELEX, S.A. Polígono Industrial Can Castells. C/ Industria, 113 nave J 08420 Canovelles Tel/ +34 93 849 17 35
Fax/ +34 93 846 78 75 e-mail: chemelex@terra.es
Ed. 10.2002

LABKIT HITACHI 704
ALBUMINE
Preparation of working reagent:
The reagent is ready to use.
Calibration:
Use LABTROL serum calibrator.
Operation conditions:
Push on the key PARAMETER JOB and introduce the following information into the programs 1, 2, and 4,
and then continue following instructions of instrument.
1- TEST NAME
TEST [.....]
TEST NAME
ALB
2- CHEMISTRY PARAMETERS
TEST [.....]
ASSAY CODE 1 - 31 - 0
SAMPLE VOLUME (µl) 3
R1 VOLUME (µl) 500 - 50 - 0
R2 VOLUME (µl) 0 - 20 - 0
WAVELENGHT 700 - 600
CALIB. METHOD 1 – 0
STD 1 CONC. RACK POS. 0.0 – 1
STD 2 CONC. RACK POS. [...] - 2
UNIT
g/dl.
SD LIMIT 0.1
DUPLICATE LIMIT 100
SENSITIVITY 0
ABS. LIMIT 0 – 0
PROZONE LIMIT 0
EXPECTED VALUE 3.5 - 5
INSTRUMENT FACTOR 1.00
4- CHANEL ASSIGNEMENT
PHASE [....]
TEST [....]
Linearity:
The method is linear until 6 g/dl.
CHEMELEX, S.A. Polígono Industrial Can Castells. C/ Industria, 113 nave J 08420 Canovelles Tel/ +34 93 849 17 35
Fax/ +34 93 846 78 75 e-mail: chemelex@terra.es
Ed. 10.2002

LABKIT HITACHI 704
UREA UV-GLDH
Preparation of working reagent:
Dissolve one vial of lyophilised R2 with a little quantity of buffer R1. Return to the original vial of buffer
when it is completely dissolved. The working reagent is stable 28 days refrigerated and keeps it out of
light.
Calibration:
Use LABTROL serum calibrator.
Operation conditions:
Push on the key PARAMETER JOB and introduce the following information into the programs 1, 2, and 4,
and then continue following instructions of instrument.
1- TEST NAME
TEST [.....]
TEST NAME
TRIG.
2- CHEMISTRY PARAMETERS
TEST [.....]
ASSAY CODE 1 - 31 - 0
SAMPLE VOLUME (µl) 4
R1 VOLUME (µl) 400 - [...] - 0
R2 VOLUME (µl) 0 - 20 - 0
WAVELENGHT 600 - 505
CALIB. METHOD 1 – 0
STD 1 CONC. RACK POS. 0.0 – 1
STD 2 CONC. RACK POS. [...] - 2
UNIT
mg/dl.
SD LIMIT 0.1
DUPLICATE LIMIT 100
SENSITIVITY 0
ABS. LIMIT 0 – 0
PROZONE LIMIT 0
EXPECTED VALUE 36 - 165
INSTRUMENT FACTOR 1.00
4- CHANEL ASSIGNEMENT
PHASE [....]
TEST [....]
Linearity:
The method is linear until 1000 mg/dl.
CHEMELEX, S.A. Polígono Industrial Can Castells. C/ Industria, 113 nave J 08420 Canovelles Tel/ +34 93 849 17 35
Fax/ +34 93 846 78 75 e-mail: chemelex@terra.es
Ed. 10.2002

LABKIT HITACHI 704
TRIGLYCERIDES
Preparation of working reagent:
Dissolve one vial of lyophilised R2 with a little quantity of buffer R1. Return to the original vial of buffer
when it is completely dissolved. The working reagent is stable 28 days refrigerated and keeps it out of
light.
Calibration:
Use LABTROL serum calibrator.
Operation conditions:
Push on the key PARAMETER JOB and introduce the following information into the programs 1, 2, and 4,
and then continue following instructions of instrument.
1- TEST NAME
TEST [.....]
TEST NAME
TRIG.
2- CHEMISTRY PARAMETERS
TEST [.....]
ASSAY CODE 1 - 31 - 0
SAMPLE VOLUME (µl) 4
R1 VOLUME (µl) 400 - [...] - 0
R2 VOLUME (µl) 0 - 20 - 0
WAVELENGHT 600 - 505
CALIB. METHOD 1 – 0
STD 1 CONC. RACK POS. 0.0 – 1
STD 2 CONC. RACK POS. [...] - 2
UNIT
mg/dl.
SD LIMIT 0.1
DUPLICATE LIMIT 100
SENSITIVITY 0
ABS. LIMIT 0 – 0
PROZONE LIMIT 0
EXPECTED VALUE 36 - 165
INSTRUMENT FACTOR 1.00
4- CHANEL ASSIGNEMENT
PHASE [....]
TEST [....]
Linearity:
The method is linear until 1000 mg/dl.
CHEMELEX, S.A. Polígono Industrial Can Castells. C/ Industria, 113 nave J 08420 Canovelles Tel/ +34 93 849 17 35
Fax/ +34 93 846 78 75 e-mail: chemelex@terra.es
Ed. 10.2002

LABKIT HITACHI 704
TOTAL PROTEINS
Preparation of working reagent:
The reagent is ready to use.
Calibration:
Use LABTROL serum calibrator.
Operation conditions:
Push on the key PARAMETER JOB and introduce the following information into the programs 1, 2, and 4,
and then continue following instructions of instrument.
1- TEST NAME
TEST [.....]
TEST NAME
TPRO
2- CHEMISTRY PARAMETERS
TEST [.....]
ASSAY CODE 1 - 31 - 0
SAMPLE VOLUME (µl) 5
R1 VOLUME (µl) 500 - 50 - 0
R2 VOLUME (µl) 0 - 50 - 0
WAVELENGHT 660 - 456
CALIB. METHOD 1 – 0
STD 1 CONC. RACK POS. 0.0 – 1
STD 2 CONC. RACK POS. [...] - 2
UNIT
g/dl.
SD LIMIT 0.1
DUPLICATE LIMIT 100
SENSITIVITY 0
ABS. LIMIT 0 – 0
PROZONE LIMIT 0
EXPECTED VALUE 6.7 - 8.7
INSTRUMENT FACTOR 1.00
4- CHANEL ASSIGNEMENT
PHASE [....]
TEST [....]
Linearity:
The method is linear until 15 g/dl.
CHEMELEX, S.A. Polígono Industrial Can Castells. C/ Industria, 113 nave J 08420 Canovelles Tel/ +34 93 849 17 35
Fax/ +34 93 846 78 75 e-mail: chemelex@terra.es
Ed. 10.2002

LABKIT HITACHI 704
TOTAL BILIRUBIN
Preparation of working reagent:
Mix 25 ml. R1 with 0.5 ml. sodium nitrite. Mix well. The working reagent is stable 3 weeks to 2 - 8ºC.
Calibration:
Use LABTROL serum calibrator.
Operation conditions:
Push on the key PARAMETER JOB and introduce the following information into the programs 1, 2, and 4,
and then continue following instructions of instrument.
1- TEST NAME
TEST [.....]
TEST NAME
BIL-T
2- CHEMISTRY PARAMETERS
TEST [.....]
ASSAY CODE 1 - 31 - 0
SAMPLE VOLUME (µl) 15
R1 VOLUME (µl) 350 - 20 - 0
R2 VOLUME (µl) 0 - 20 - 0
WAVELENGHT 660 - 546
CALIB. METHOD 1 – 0
STD 1 CONC. RACK POS. 0.0 – 1
STD 2 CONC. RACK POS. [...] - 2
UNIT
g/dl.
SD LIMIT 0.1
DUPLICATE LIMIT 100
SENSITIVITY 0
ABS. LIMIT 0 – 0
PROZONE LIMIT 0
EXPECTED VALUE 0.0 - 1.1
INSTRUMENT FACTOR 1.00
4- CHANEL ASSIGNEMENT
PHASE [....]
TEST [....]
Linearity:
The method is linear until 30 mg/dl.
CHEMELEX, S.A. Polígono Industrial Can Castells. C/ Industria, 113 nave J 08420 Canovelles Tel/ +34 93 849 17 35
Fax/ +34 93 846 78 75 e-mail: chemelex@terra.es
Ed. 10.2002

LABKIT HITACHI 704
RF Turbidymetric
Preparation of working reagent:
Follow instructions given on the procedure.
Calibration:
Use RF CALIBRATOR.
Operation conditions:
Non-Linear Calibration.
1- TEST NAME
TEST [.....]
TEST NAME
RF
2- CHEMISTRY PARAMETERS
TEST [.....]
ASSAY CODE [2 POINTS] - 20 - 32
SAMPLE VOLUME (µl) 3
R1 VOLUME (µl)
350 - 20 - NO
R2 VOLUME (µl)
40 - 20 - NO
WAVELENGHT 600
CALIB. METHOD NON-LINEAR 1 - 5
STD 1 CONC. RACK POS. 0.0 – 1
STD 2 CONC. RACK POS. 16.5 - 2
STD 3 CONC. RACK POS. 33 – 3
STD 4 CONC. RACK POS. 66 – 4
STD 5 CONC. RACK POS. 132 – 5
STD 6 CONC. RACK POS. 0.0 – 6
UNIT
IU/ml.
SD LIMIT 300
DUPLICATE LIMIT 1000
SENSITIVITY 0
ABS. LIMIT
0 - INCREASE
PROZONE LIMIT
0 - LOWER
EXPECTED VALUE 0 - 20
INSTRUMENT FACTOR 1.0
*Depends on the concentration of calibrated used.
CHEMELEX, S.A. Polígono Industrial Can Castells. C/ Industria, 113 nave J 08420 Canovelles Tel/ +34 93 849 17 35
Fax/ +34 93 846 78 75 e-mail: chemelex@terra.es
Ed. 10.2002

LABKIT HITACHI 704
PHOSPHORUS
Preparation of working reagent:
The reagents are ready to use.
Calibration:
Use LABTROL serum calibrator.
Operation conditions:
Push on the key PARAMETER JOB and introduce the following information into the programs 1, 2, and 4,
and then continue following instructions of instrument.
1- TEST NAME
TEST [.....]
TEST NAME
PHOSPH
2- CHEMISTRY PARAMETERS
TEST [.....]
ASSAY CODE 1 - 32 – 0
SAMPLE VOLUME (µl) 7
R1 VOLUME (µl) 350 - 50 - 0
R2 VOLUME (µl) 0 - 50 - 0
WAVELENGHT 376 - 340
CALIB. METHOD 1 – 0
STD 1 CONC. RACK POS. 0.0 – 1
STD 2 CONC. RACK POS. [...] - 2
UNIT
mg/dl.
SD LIMIT 0.1
DUPLICATE LIMIT 100
SENSITIVITY 0
ABS. LIMIT 0 – 0
PROZONE LIMIT 0
EXPECTED VALUE 2.5 - 5.0
INSTRUMENT FACTOR 1.00
4- CHANEL ASSIGNEMENT
PHASE [....]
TEST [....]
Linearity:
The method is linear until 15 mg/dl.
CHEMELEX, S.A. Polígono Industrial Can Castells. C/ Industria, 113 nave J 08420 Canovelles Tel/ +34 93 849 17 35
Fax/ +34 93 846 78 75 e-mail: chemelex@terra.es
Ed. 10.2002

LABKIT HITACHI 704
PHOSPHOLIPIDES
Preparation of working reagent:
Dissolve 1 vial lyophilised R2 with 10 ml. buffer R1. Homogenise. The working reagent is stable 2 weeks
refrigerated and keeps it out of the light.
Calibration:
Use LABTROL serum calibrator.
Operation conditions:
Push on the key PARAMETER JOB and introduce the following information into the programs 1, 2, and 4,
and then continue following instructions of instrument.
1- TEST NAME
TEST [.....]
TEST NAME
PHLPD
2- CHEMISTRY PARAMETERS
TEST [.....]
ASSAY CODE 1 - 31 - 0
SAMPLE VOLUME (µl) 4
R1 VOLUME (µl) 350 - 50 - 0
R2 VOLUME (µl) 0 - 50 - 0
WAVELENGHT 600 - 505
CALIB. METHOD 1 – 0
STD 1 CONC. RACK POS. 0.0 – 1
STD 2 CONC. RACK POS. [...] - [...]
UNIT
mg/dl.
SD LIMIT 0.1
DUPLICATE LIMIT 100
SENSITIVITY 0
ABS. LIMIT 0 – 0
PROZONE LIMIT 0
EXPECTED VALUE 160 - 270
INSTRUMENT FACTOR 1.00
4- CHANEL ASSIGNEMENT
PHASE [....]
TEST [....]
Linearity:
The method is linear until 600 mg/dl.
CHEMELEX, S.A. Polígono Industrial Can Castells. C/ Industria, 113 nave J 08420 Canovelles Tel/ +34 93 849 17 35
Fax/ +34 93 846 78 75 e-mail: chemelex@terra.es
Ed. 10.2002

LABKIT HITACHI 704
MAGNESIUM
Preparation of working reagent:
The reagent is ready to use.
Calibration:
Use LABTROL serum calibrator.
Operation conditions:
Push on the key PARAMETER JOB and introduce the following information into the programs 1, 2, and 4,
and then continue following instructions of instrument.
1- TEST NAME
TEST [.....]
TEST NAME
MG
2- CHEMISTRY PARAMETERS
TEST [.....]
ASSAY CODE 2 - 15 - 32
SAMPLE VOLUME (µl) 5
R1 VOLUME (µl) 200 - 100 - 0
R2 VOLUME (µl) 200 - 100 - 0
WAVELENGHT 600 - 505
CALIB. METHOD 1 – 0
STD 1 CONC. RACK POS. 0.0 – 1
STD 2 CONC. RACK POS. [...] - 2
UNIT
mg/dl.
SD LIMIT 0.1
DUPLICATE LIMIT 100
SENSITIVITY 0
ABS. LIMIT 0 – 0
PROZONE LIMIT 0
EXPECTED VALUE 1.6 - 2.5
INSTRUMENT FACTOR 1.00
4- CHANEL ASSIGNEMENT
PHASE [....]
TEST [....]
Linearity:
The method is linear until 6 mg/dl.
CHEMELEX, S.A. Polígono Industrial Can Castells. C/ Industria, 113 nave J 08420 Canovelles Tel/ +34 93 849 17 35
Fax/ +34 93 846 78 75 e-mail: chemelex@terra.es
Ed. 10.2002

LABKIT HITACHI 704
Lp(a) Turbidymetric
Preparation of working reagent:
Follow instructions given on the procedure.
Calibration:
Use Lp(a) CALIBRATOR.
Operation conditions:
Non-Linear Calibration.
1- TEST NAME
TEST [.....]
TEST NAME
Lp(a)
2- CHEMISTRY PARAMETERS
TEST [.....]
ASSAY CODE [2 POINTS] - 20 - 32
SAMPLE VOLUME (µl) 3
R1 VOLUME (µl)
350 - 20 - NO
R2 VOLUME (µl)
40 - 20 - NO
WAVELENGHT 600
CALIB. METHOD NON-LINEAR 1 - 5
STD 1 CONC. RACK POS. 0.0 – 1
STD 2 CONC. RACK POS. 100 - 2
STD 3 CONC. RACK POS. 200 - 3
STD 4 CONC. RACK POS. 400 - 4
STD 5 CONC. RACK POS. 800 - 5
STD 6 CONC. RACK POS. 0.0 - 6
UNIT
mg/ml.
SD LIMIT 300
DUPLICATE LIMIT 1000
SENSITIVITY 0
ABS. LIMIT
0 - INCREASE
PROZONE LIMIT
0 - LOWER
EXPECTED VALUE 0 - 300
INSTRUMENT FACTOR 1.0
CHEMELEX, S.A. Polígono Industrial Can Castells. C/ Industria, 113 nave J 08420 Canovelles Tel/ +34 93 849 17 35
Fax/ +34 93 846 78 75 e-mail: chemelex@terra.es
Ed. 10.2002

LABKIT HITACHI 704
LDH
Preparation of working reagent:
Dissolve one tablet with 15 ml. of buffer. The working reagent is stable 3 days refrigerated.
Calibration:
Use LABTROL serum calibrator.
Operation conditions:
Push on the key PARAMETER JOB and introduce the following information into the programs 1, 2, and 4,
and then continue following instructions of instrument.
1- TEST NAME
TEST [.....]
TEST NAME
LDH
2- CHEMISTRY PARAMETERS
TEST [.....]
ASSAY CODE 5 - 5 - 12
SAMPLE VOLUME (µl) 10
R1 VOLUME (µl) 350 - [...] - 0
R2 VOLUME (µl) 0 - 20 - 0
WAVELENGHT 376 - 340
CALIB. METHOD 2 - 0
STD 1 CONC. RACK POS. 0 – 1
UNIT
U/L.
SD LIMIT 0.1
DUPLICATE LIMIT 100
SENSITIVITY 0
ABS. LIMIT 5000 - 1
PROZONE LIMIT 0
EXPECTED VALUE 0 - 460
INSTRUMENT FACTOR 1.00
3-CALIBRATION MONITOR
K=-8571
4- CHANEL ASSIGNEMENT
PHASE [....]
TEST [....]
Linearity:
The method is linear until 1500 U/L.
CHEMELEX, S.A. Polígono Industrial Can Castells. C/ Industria, 113 nave J 08420 Canovelles Tel/ +34 93 849 17 35
Fax/ +34 93 846 78 75 e-mail: chemelex@terra.es
Ed. 10.2002

LABKIT HITACHI 704
LACTATE
Preparation of working reagent:
Dissolve 1 vial lyophilised R2 with 10 ml of buffer R1. Homogenise. The working reagent is stable 4 weeks
2-8ºC. and keep it out of the light.
Calibration:
Use LABTROL serum calibrator.
Operation conditions:
Push on the key PARAMETER JOB and introduce the following information into the programs 1, 2, and 4,
and then continue following instructions of instrument.
1- TEST NAME
TEST [.....]
TEST NAME
LACT
2- CHEMISTRY PARAMETERS
TEST [.....]
ASSAY CODE 1 - 31 – 0
SAMPLE VOLUME (µl) 4
R1 VOLUME (µl) 350 - 20 - 0
R2 VOLUME (µl) 0 - 20 - 0
WAVELENGHT 600 - 505
CALIB. METHOD 1 – 0
STD 1 CONC. RACK POS. 0.0 – 1
STD 2 CONC. RACK POS. [...] - [...]
UNIT
mg/dl.
SD LIMIT 0.1
DUPLICATE LIMIT 100
SENSITIVITY 0
ABS. LIMIT 0 – 0
PROZONE LIMIT 0
EXPECTED VALUE 9.0 - 16.0
INSTRUMENT FACTOR 1.00
4- CHANEL ASSIGNEMENT
PHASE [....]
TEST [....]
Linearity:
The method is linear until 150 mg/dl.
CHEMELEX, S.A. Polígono Industrial Can Castells. C/ Industria, 113 nave J 08420 Canovelles Tel/ +34 93 849 17 35
Fax/ +34 93 846 78 75 e-mail: chemelex@terra.es
Ed. 10.2002

LABKIT HITACHI 704
HDL-CHOLESTEROL
Preparation of working reagent:
Precipitated reagent.
Calibration:
Use LABTROL serum calibrator.
Operation conditions:
Make separation of HDL fraction, following the procedure instructions.
Push on the key PARAMETER JOB and introduce the following information into the programs 1, 2, and 4,
and then continue following instructions of instrument.
1- TEST NAME
TEST [.....]
TEST NAME
HDL
2- CHEMISTRY PARAMETERS
TEST [.....]
ASSAY CODE 1 - 31 - 0
SAMPLE VOLUME (µl) 10
R1 VOLUME (µl) 300 - 20 - 0
R2 VOLUME (µl) 0 - 20 - 0
WAVELENGHT 700 - 505
CALIB. METHOD 1 – 0
STD 1 CONC. RACK POS. 0.0 – 1
STD 2 CONC. RACK POS. [...] - [...]
UNIT
mg/dl.
SD LIMIT 0.1
DUPLICATE LIMIT 100
SENSITIVITY 0
ABS. LIMIT 0 – 0
PROZONE LIMIT 0
EXPECTED VALUE 30 - 65
INSTRUMENT FACTOR 1.00
4- CHANEL ASSIGNEMENT
PHASE [....]
TEST [....]
Linearity:
The method is linear until 300 mg/dl.
CHEMELEX, S.A. Polígono Industrial Can Castells. C/ Industria, 113 nave J 08420 Canovelles Tel/ +34 93 849 17 35
Fax/ +34 93 846 78 75 e-mail: chemelex@terra.es
Ed. 10.2002

LABKIT HITACHI 704
GPT / ALT
Preparation of working reagent:
Dissolve one tablet into one vial of buffer or one tablet with 15 ml. of buffer, depending of presentation.
The working reagent is stable 2 weeks refrigerated.
Calibration:
Use LABTROL serum calibrator.
Operation conditions:
Push on the key PARAMETER JOB and introduce the following information into the programs 1, 2, and 4,
and then continue following instructions of instrument.
1- TEST NAME
TEST [.....]
TEST NAME
GPT
2- CHEMISTRY PARAMETERS
TEST [.....]
ASSAY CODE 5 - 5 - 15
SAMPLE VOLUME (µl) 20
R1 VOLUME (µl) 350 - 50 - 0
R2 VOLUME (µl) 0 - 20 - 0
WAVELENGHT 376 - 340
CALIB. METHOD 2 - 0
STD 1 CONC. RACK POS. 0 – 1
UNIT
U/L.
SD LIMIT 0.1
DUPLICATE LIMIT 100
SENSITIVITY 0
ABS. LIMIT 5000 - 1
PROZONE LIMIT 0
EXPECTED VALUE 0 - 40
INSTRUMENT FACTOR 1.00
3-CALIBRATION MONITOR
K=3500
4- CHANEL ASSIGNEMENT
PHASE [....]
TEST [....]
Linearity:
The method is linear until 500 U/L.
CHEMELEX, S.A. Polígono Industrial Can Castells. C/ Industria, 113 nave J 08420 Canovelles Tel/ +34 93 849 17 35
Fax/ +34 93 846 78 75 e-mail: chemelex@terra.es
Ed. 10.2002

LABKIT HITACHI 704
GOT / AST
Preparation of working reagent:
Dissolve one tablet into one vial of buffer or one tablet with 15 ml. of buffer, depending of presentation.
The working reagent is stable 2 weeks refrigerated.
Calibration:
Use LABTROL serum calibrator.
Operation conditions:
Push on the key PARAMETER JOB and introduce the following information into the programs 1, 2, and 4,
and then continue following instructions of instrument.
1- TEST NAME
TEST [.....]
TEST NAME
GOT
2- CHEMISTRY PARAMETERS
TEST [.....]
ASSAY CODE 5 - 5 - 15
SAMPLE VOLUME (µl) 20
R1 VOLUME (µl) 350 - 50 - 0
R2 VOLUME (µl) 0 - 20 - 0
WAVELENGHT 376 - 340
CALIB. METHOD 2 - 0
STD 1 CONC. RACK POS. 0 – 1
UNIT
U/L.
SD LIMIT 0.1
DUPLICATE LIMIT 100
SENSITIVITY 0
ABS. LIMIT 5000 - 1
PROZONE LIMIT 0
EXPECTED VALUE 0 - 35
INSTRUMENT FACTOR 1.00
3-CALIBRATION MONITOR
K=3500
4- CHANEL ASSIGNEMENT
PHASE [....]
TEST [....]
Linearity:
The method is linear until 500 U/L.
CHEMELEX, S.A. Polígono Industrial Can Castells. C/ Industria, 113 nave J 08420 Canovelles Tel/ +34 93 849 17 35
Fax/ +34 93 846 78 75 e-mail: chemelex@terra.es
Ed. 10.2002

LABKIT HITACHI 704
GLUCOSE Hexokinase
Preparation of working reagent:
Dissolve 1 vial lyophilised R2 with a little quantity of buffer R1. Return to the original vial of buffer when it is
completely dissolved. The working reagent is stable 1 month refrigerated and keep it out of the light.
Calibration:
Use LABTROL serum calibrator.
Operation conditions:
Push on the key PARAMETER JOB and introduce the following information into the programs 1, 2, and 4,
and then continue following instructions of instrument.
1- TEST NAME
TEST [.....]
TEST NAME
GLU-H
2- CHEMISTRY PARAMETERS
TEST [.....]
ASSAY CODE 1 - 31 – 0
SAMPLE VOLUME (µl) 3
R1 VOLUME (µl) 300 - 100 - 0
R2 VOLUME (µl) 0 - 20 - 0
WAVELENGHT 405 - 340
CALIB. METHOD 1 – 0
STD 1 CONC. RACK POS. 0.0 – 1
STD 2 CONC. RACK POS. [...] - 2
UNIT
mg/dl.
SD LIMIT 0.1
DUPLICATE LIMIT 100
SENSITIVITY 0
ABS. LIMIT 0 – 0
PROZONE LIMIT 0
PANIC VALUE [...] - [...]
EXPECTED VALUE 60 - 110
INSTRUMENT FACTOR 1.00
4- CHANEL ASSIGNEMENT
PHASE [....]
TEST [....]
Linearity:
The method is linear until 700 mg/dl.
CHEMELEX, S.A. Polígono Industrial Can Castells. C/ Industria, 113 nave J 08420 Canovelles Tel/ +34 93 849 17 35
Fax/ +34 93 846 78 75 e-mail: chemelex@terra.es
Ed. 10.2002

LABKIT HITACHI 704
GLUCOSE
Preparation of working reagent:
Dissolve 1 vial lyophilised R2 with a little quantity of buffer R1. Return to the original vial of buffer when it is
completely dissolved. The working reagent is stable 1 month refrigerated and keep it out of the light.
Calibration:
Use LABTROL serum calibrator.
Operation conditions:
Push on the key PARAMETER JOB and introduce the following information into the programs 1, 2, and 4,
and then continue following instructions of instrument.
1- TEST NAME
TEST [.....]
TEST NAME
GLU
2- CHEMISTRY PARAMETERS
TEST [.....]
ASSAY CODE 1 - 31 – 0
SAMPLE VOLUME (µl) 4
R1 VOLUME (µl) 400 - 50 - 0
R2 VOLUME (µl) 0 - 20 - 0
WAVELENGHT 600 - 505
CALIB. METHOD 1 – 0
STD 1 CONC. RACK POS. 0.0 – 1
STD 2 CONC. RACK POS. [...] - 2
UNIT
mg/dl.
SD LIMIT 0.1
DUPLICATE LIMIT 100
SENSITIVITY 0
ABS. LIMIT 0 – 0
PROZONE LIMIT 0
EXPECTED VALUE 60 - 110
INSTRUMENT FACTOR 1.00
4- CHANEL ASSIGNEMENT
PHASE [....]
TEST [....]
Linearity:
The method is linear until 500 mg/dl.
CHEMELEX, S.A. Polígono Industrial Can Castells. C/ Industria, 113 nave J 08420 Canovelles Tel/ +34 93 849 17 35
Fax/ +34 93 846 78 75 e-mail: chemelex@terra.es
Ed. 10.2002

LABKIT HITACHI 704
γ-GT
Preparation of working reagent:
Dissolve one tablet into one vial of buffer or one tablet with 15 ml. of buffer, depending of presentation.
The working reagent is stable 21 days refrigerated.
Calibration:
Use LABTROL serum calibrator.
Operation conditions:
Push on the key PARAMETER JOB and introduce the following information into the programs 1, 2, and 4,
and then continue following instructions of instrument.
1- TEST NAME
TEST [.....]
TEST NAME
GGT
2- CHEMISTRY PARAMETERS
TEST [.....]
ASSAY CODE 5 - 5 - 12
SAMPLE VOLUME (µl) 20
R1 VOLUME (µl) 350 - 50 - 0
R2 VOLUME (µl) 0 - 20 - 0
WAVELENGHT 660 - 415
CALIB. METHOD 2 - 0
STD 1 CONC. RACK POS. 0 – 1
UNIT
U/L.
SD LIMIT 0.1
DUPLICATE LIMIT 100
SENSITIVITY 0
ABS. LIMIT 15000 - 1
PROZONE LIMIT 0
EXPECTED VALUE 0 - 50
INSTRUMENT FACTOR 1.00
3-CALIBRATION MONITOR
K=3000
4- CHANEL ASSIGNEMENT
PHASE [....]
TEST [....]
Linearity:
The method is linear until 500 U/L.
CHEMELEX, S.A. Polígono Industrial Can Castells. C/ Industria, 113 nave J 08420 Canovelles Tel/ +34 93 849 17 35
Fax/ +34 93 846 78 75 e-mail: chemelex@terra.es
Ed. 10.2002

LABKIT HITACHI 704
FERRITIN Turbidymetric
Preparation of working reagent:
Follow instructions given on the procedure.
Calibration:
Use FERRITIN CALIBRATOR.
Operation conditions:
Non-Linear Calibration.
1- TEST NAME
TEST [.....]
TEST NAME
FERRITIN
2- CHEMISTRY PARAMETERS
TEST [.....]
ASSAY CODE [2 POINTS] - 19 - 32
SAMPLE VOLUME (µl) 20
R1 VOLUME (µl)
230 - 20 - NO
R2 VOLUME (µl)
85 - 20 - NO
WAVELENGHT 600
CALIB. METHOD NON-LINEAR 1 - 5
STD 1 CONC. RACK POS. 0.0 – 1
STD 2 CONC. RACK POS. 87.4 - 2
STD 3 CONC. RACK POS. 160 - 3
STD 4 CONC. RACK POS. 314 - 4
STD 5 CONC. RACK POS. 558 - 5
STD 6 CONC. RACK POS. 0.0 - 6
UNIT
ng/ml.
SD LIMIT 300
DUPLICATE LIMIT 500
SENSITIVITY 0
ABS. LIMIT
0 - INCREASE
PROZONE LIMIT
0 - LOWER
EXPECTED VALUE 15 - 300
INSTRUMENT FACTOR 1.0
CHEMELEX, S.A. Polígono Industrial Can Castells. C/ Industria, 113 nave J 08420 Canovelles Tel/ +34 93 849 17 35
Fax/ +34 93 846 78 75 e-mail: chemelex@terra.es
Ed. 10.2002

LABKIT HITACHI 704
CRP Turbidymetric
Preparation of working reagent:
Follow instructions given on the procedure.
Calibration:
Use CRP CALIBRATOR.
Operation conditions:
Non-Linear Calibration.
1- TEST NAME
TEST [.....]
TEST NAME
CRP
2- CHEMISTRY PARAMETERS
TEST [.....]
ASSAY CODE [2 POINTS] - 20 - 32
SAMPLE VOLUME (µl) 6
R1 VOLUME (µl) 350
R2 VOLUME (µl) 50
WAVELENGHT 505
CALIB. METHOD NON-LINEAR 1 - 5
STD 1 CONC. RACK POS. 0.0 – 1
STD 2 CONC. RACK POS. 5 - 2
STD 3 CONC. RACK POS. 10 - 3
STD 4 CONC. RACK POS. 20 - 4
STD 5 CONC. RACK POS. 40 - 5
STD 6 CONC. RACK POS. 0.0 - 6
UNIT
mg/l.
SD LIMIT 300
DUPLICATE LIMIT 1000
SENSITIVITY 0
ABS. LIMIT
0 - INCREASE
PROZONE LIMIT
0 - LOWER
EXPECTED VALUE 0 - 8
INSTRUMENT FACTOR 1.0
#Enhanced sensitivity (i.e. paediatric samples).
CHEMELEX, S.A. Polígono Industrial Can Castells. C/ Industria, 113 nave J 08420 Canovelles Tel/ +34 93 849 17 35
Fax/ +34 93 846 78 75 e-mail: chemelex@terra.es
Ed. 10.2002

LABKIT HITACHI 704
CRP Turbidymetric
Preparation of working reagent:
Follow instructions given on the procedure.
Calibration:
Use CRP CALIBRATOR.
Operation conditions:
Linear Calibration.
1- TEST NAME
TEST [.....]
TEST NAME
CRP
2- CHEMISTRY PARAMETERS
TEST [.....]
ASSAY CODE [2 POINTS] - 20 - 32
SAMPLE VOLUME (µl) 3
R1 VOLUME (µl)
350 - 20 - NO
R2 VOLUME (µl)
50 - 20 - NO
WAVELENGHT 505
CALIB. METHOD NON-LINEAR 1 - 5
STD 1 CONC. RACK POS. 0.0 – 1
STD 2 CONC. RACK POS. 10 - 2
STD 3 CONC. RACK POS. 20 - 3
STD 4 CONC. RACK POS. 40 - 4
STD 5 CONC. RACK POS. 80 - 5
STD 6 CONC. RACK POS. 0.0 - 6
UNIT
mg/l.
SD LIMIT 300
DUPLICATE LIMIT 1000
SENSITIVITY 0
ABS. LIMIT
0 - INCREASE
PROZONE LIMIT
0 - LOWER
EXPECTED VALUE 0 - 8
INSTRUMENT FACTOR 1.0
CHEMELEX, S.A. Polígono Industrial Can Castells. C/ Industria, 113 nave J 08420 Canovelles Tel/ +34 93 849 17 35
Fax/ +34 93 846 78 75 e-mail: chemelex@terra.es
Ed. 10.2002

LABKIT HITACHI 704
CREATININE
Preparation of working reagent:
The reagents are ready to use. Alkaline reagent is used as reagent 1 and picric reagent is used as reagent
2.
Calibration:
Use LABTROL serum calibrator.
Operation conditions:
Push on the key PARAMETER JOB and introduce the following information into the programs 1, 2, and 4,
and then continue following instructions of instrument.
1- TEST NAME
TEST [.....]
TEST NAME
CREA
2- CHEMISTRY PARAMETERS
TEST [.....]
ASSAY CODE 5 - 19 - 21
SAMPLE VOLUME (µl) 20
R1 VOLUME (µl) 175 - 50 - 0
R2 VOLUME (µl) 175 - 50 - 0
WAVELENGHT 600 - 505
CALIB. METHOD 1 – 0
STD 1 CONC. RACK POS. 0.0 – 1
STD 2 CONC. RACK POS. [...] - 2
UNIT
mg/dl.
SD LIMIT 0.1
DUPLICATE LIMIT 100
SENSITIVITY 0
ABS. LIMIT 10000 - 0
PROZONE LIMIT 0
EXPECTED VALUE 0.7 - 1.4
INSTRUMENT FACTOR 1.00
4- CHANEL ASSIGNEMENT
PHASE [....]
TEST [....]
Linearity:
The method is linear until 15 mg/dl.
CHEMELEX, S.A. Polígono Industrial Can Castells. C/ Industria, 113 nave J 08420 Canovelles Tel/ +34 93 849 17 35
Fax/ +34 93 846 78 75 e-mail: chemelex@terra.es
Ed. 10.2002

LABKIT HITACHI 704
CHOLINESTERASE
Preparation of working reagent:
Dissolve one tablet into one vial of buffer. The working reagent is stable 2 hours refrigerated.
Calibration:
Use LABTROL serum calibrator.
Operation conditions:
Push on the key PARAMETER JOB and introduce the following information into the programs 1, 2, and 4,
and then continue following instructions of instrument.
1- TEST NAME
TEST [.....]
TEST NAME
COLIN
2- CHEMISTRY PARAMETERS
TEST [.....]
ASSAY CODE 5 - 5 - 9
SAMPLE VOLUME (µl) 2
R1 VOLUME (µl) 500 - 20 - 0
R2 VOLUME (µl) 0 - 50 - 0
WAVELENGHT 660 - 415
CALIB. METHOD 1 – 0
STD 1 CONC. RACK POS. 0.0 – 1
STD 2 CONC. RACK POS. [...] - 2
UNIT
U/L.
SD LIMIT 0.1
DUPLICATE LIMIT 100
SENSITIVITY 0
ABS. LIMIT 20000 - 0
PROZONE LIMIT 0
EXPECTED VALUE 4659 - 14443
INSTRUMENT FACTOR 1.00
4- CHANEL ASSIGNEMENT
PHASE [....]
TEST [....]
Linearity:
The method is linear until 15000 U/L.
CHEMELEX, S.A. Polígono Industrial Can Castells. C/ Industria, 113 nave J 08420 Canovelles Tel/ +34 93 849 17 35
Fax/ +34 93 846 78 75 e-mail: chemelex@terra.es
Ed. 10.2002

LABKIT HITACHI 704
CHOLESTEROL
Preparation of working reagent:
Dissolve one vial R2 with a little quantity of buffer. Return to the original vial when it is completely
dissolved. The working reagent is stable 4 months refrigerated and out of the light.
Calibration:
Use LABTROL serum calibrator.
Operation conditions:
Push on the key PARAMETER JOB and introduce the following information into the programs 1, 2, and 4,
and then continue following instructions of instrument.
1- TEST NAME
TEST [.....]
TEST NAME
CHOL
2- CHEMISTRY PARAMETERS
TEST [.....]
ASSAY CODE 1 - 31 - 0
SAMPLE VOLUME (µl) 4
R1 VOLUME (µl) 400 - 50 - 0
R2 VOLUME (µl) 0 - 20 - 0
WAVELENGHT 600 - 505
CALIB. METHOD 1 – 0
STD 1 CONC. RACK POS. 0.0 – 1
STD 2 CONC. RACK POS. [...] - 2
UNIT
mg/dl.
SD LIMIT 0.1
DUPLICATE LIMIT 100
SENSITIVITY 0
ABS. LIMIT 0 – 0
PROZONE LIMIT 0
EXPECTED VALUE 120 - 260
INSTRUMENT FACTOR 1.00
4- CHANEL ASSIGNEMENT
PHASE [....]
TEST [....]
Linearity:
The method is linear until 600 mg/dl.
CHEMELEX, S.A. Polígono Industrial Can Castells. C/ Industria, 113 nave J 08420 Canovelles Tel/ +34 93 849 17 35
Fax/ +34 93 846 78 75 e-mail: chemelex@terra.es
Ed. 10.2002

LABKIT HITACHI 704
CHLORIDE
Preparation of working reagent:
The reagent is ready to use.
Calibration:
Use LABTROL serum calibrator.
Operation conditions:
Push on the key PARAMETER JOB and introduce the following information into the programs 1, 2, and 4,
and then continue following instructions of instrument.
1- TEST NAME
TEST [.....]
TEST NAME
CLOR
2- CHEMISTRY PARAMETERS
TEST [.....]
ASSAY CODE 1 - 31 - 0
SAMPLE VOLUME (µl) 5
R1 VOLUME (µl) 400 - 50 - 0
R2 VOLUME (µl) 0 - 20 - 0
WAVELENGHT 660 - 505
CALIB. METHOD 1 – 0
STD 1 CONC. RACK POS. 0.0 – 1
STD 2 CONC. RACK POS. [...] - 2
UNIT
mEQ/L.
SD LIMIT 0.1
DUPLICATE LIMIT 100
SENSITIVITY 0
ABS. LIMIT 0 – 0
PROZONE LIMIT 0
EXPECTED VALUE 98 - 107
INSTRUMENT FACTOR 1.00
4- CHANEL ASSIGNEMENT
PHASE [....]
TEST [....]
Linearity:
The method is linear until 130 mEQ/L.
CHEMELEX, S.A. Polígono Industrial Can Castells. C/ Industria, 113 nave J 08420 Canovelles Tel/ +34 93 849 17 35
Fax/ +34 93 846 78 75 e-mail: chemelex@terra.es
Ed. 10.2002

LABKIT HITACHI 704
CALCIUM OCC
Preparation of working reagent:
The reagents are ready to use. Buffer is used as R1 and colour solution as R2.
Calibration:
Use LABTROL serum calibrator.
Operation conditions:
Push on the key PARAMETER JOB and introduce the following information into the programs 1, 2, and 4,
and then continue following instructions of instrument.
1- TEST NAME
TEST [.....]
TEST NAME
CALC
2- CHEMISTRY PARAMETERS
TEST [.....]
ASSAY CODE 2 - 15 - 32
SAMPLE VOLUME (µl) 5
R1 VOLUME (µl) 250 - [...] - 0
R2 VOLUME (µl) 250 - [...] - 0
WAVELENGHT 660 - 570
CALIB. METHOD 1 – 0
STD 1 CONC. RACK POS. 0.0 – 1
STD 2 CONC. RACK POS. [...] - 2
UNIT
mg/dl.
SD LIMIT 0.1
DUPLICATE LIMIT 100
SENSITIVITY 0
ABS. LIMIT 0 – 0
PROZONE LIMIT 0
EXPECTED VALUE 9.0 - 11.0
INSTRUMENT FACTOR 1.00
4- CHANEL ASSIGNEMENT
PHASE [....]
TEST [....]
Linearity:
The method is linear until 15 mg/dl.
CHEMELEX, S.A. Polígono Industrial Can Castells. C/ Industria, 113 nave J 08420 Canovelles Tel/ +34 93 849 17 35
Fax/ +34 93 846 78 75 e-mail: chemelex@terra.es
Ed. 10.2002

LABKIT HITACHI 704
β2-Microglobulin Turbidymetric
Preparation of working reagent:
Follow instructions given on the procedure.
Calibration:
Use Beta-2-Microg. CALIBRATOR.
Operation conditions:
Non-Linear Calibration.
1- TEST NAME
TEST [.....]
TEST NAME
BETA2
2- CHEMISTRY PARAMETERS
TEST [.....]
ASSAY CODE [2 POINTS] - 20 - 32
SAMPLE VOLUME (µl) 3
R1 VOLUME (µl)
350 - 20 - NO
R2 VOLUME (µl)
50 - 20 - NO
WAVELENGHT 600
CALIB. METHOD NON-LINEAR 1 - 5
STD 1 CONC. RACK POS. 0.0 – 1
STD 2 CONC. RACK POS. 0.68 - 2
STD 3 CONC. RACK POS. 1.35 - 3
STD 4 CONC. RACK POS. 2.70 - 4
STD 5 CONC. RACK POS. 5.40 - 5
STD 6 CONC. RACK POS. 10.8 - 6
UNIT
mg/l.
SD LIMIT 300
DUPLICATE LIMIT 1000
SENSITIVITY 0
ABS. LIMIT
0 - INCREASE
PROZONE LIMIT
0 - LOWER
EXPECTED VALUE 0.8 - 2.4
INSTRUMENT FACTOR 1.0
CHEMELEX, S.A. Polígono Industrial Can Castells. C/ Industria, 113 nave J 08420 Canovelles Tel/ +34 93 849 17 35
Fax/ +34 93 846 78 75 e-mail: chemelex@terra.es
Ed. 10.2002

LABKIT HITACHI 704
ASO Turbidymetric
Preparation of working reagent:
Follow instructions given on the procedure.
Calibration:
Use ASO CALIBRATOR.
Operation conditions:
Non-Linear Calibration.
1- TEST NAME
TEST [.....]
TEST NAME
ASO
2- CHEMISTRY PARAMETERS
TEST [.....]
ASSAY CODE [2 POINTS] - 20 - 32
SAMPLE VOLUME (µl) 3
R1 VOLUME (µl)
350 - 20 - NO
R2 VOLUME (µl)
40 - 20 - NO
WAVELENGHT 505
CALIB. METHOD NON-LINEAR 1 - 5
STD 1 CONC. RACK POS. 0.0 – 1
STD 2 CONC. RACK POS. 100 - 2*
STD 3 CONC. RACK POS. 200 – 3
STD 4 CONC. RACK POS. 400 – 4
STD 5 CONC. RACK POS. 800 – 5
STD 6 CONC. RACK POS. 0.0 – 6
UNIT
IU/ml.
SD LIMIT 300
DUPLICATE LIMIT 1000
SENSITIVITY 0
ABS. LIMIT
0 - INCREASE
PROZONE LIMIT
0 - LOWER
EXPECTED VALUE 0 - 250
INSTRUMENT FACTOR 1.0
(*) Dilute standard ASO 1+1 with 0.9% NaCl saline solution to give a standard concentration of 400 IU/ml.
CHEMELEX, S.A. Polígono Industrial Can Castells. C/ Industria, 113 nave J 08420 Canovelles Tel/ +34 93 849 17 35
Fax/ +34 93 846 78 75 e-mail: chemelex@terra.es
Ed. 10.2002

LABKIT HITACHI 704
ASO Turbidymetric
Preparation of working reagent:
Follow instructions given on the procedure.
Calibration:
Use ASO CALIBRATOR.
Operation conditions:
Linear Calibration.
1- TEST NAME
TEST [.....]
TEST NAME
ASO
2- CHEMISTRY PARAMETERS
TEST [.....]
ASSAY CODE [2 POINTS] - 20 - 32
SAMPLE VOLUME (µl) 3
R1 VOLUME (µl)
350 - 20 - NO
R2 VOLUME (µl)
40 - 20 - NO
WAVELENGHT 505
CALIB. METHOD LINEAR 0 - 0
STD 1 CONC. RACK POS. 0.0 – 1
STD 2 CONC. RACK POS. 400 - 2*
STD 3 CONC. RACK POS. 0.0 – 3
STD 4 CONC. RACK POS. 0.0 – 4
STD 5 CONC. RACK POS. 0.0 – 5
STD 6 CONC. RACK POS. 0.0 – 6
UNIT
IU/ml.
SD LIMIT 300
DUPLICATE LIMIT 1000
SENSITIVITY 0
ABS. LIMIT
0 - INCREASE
PROZONE LIMIT
0 - LOWER
EXPECTED VALUE 0 - 250
INSTRUMENT FACTOR 1.0
(*) Dilute standard ASO 1+1 with 0.9% NaCl saline solution to give a standard concentration of 400 IU/ml.
CHEMELEX, S.A. Polígono Industrial Can Castells. C/ Industria, 113 nave J 08420 Canovelles Tel/ +34 93 849 17 35
Fax/ +34 93 846 78 75 e-mail: chemelex@terra.es
Ed. 10.2002

LABKIT HITACHI 704
ALKALINE PHOSPHATASE
Preparation of working reagent:
Dissolve one tablet into one vial of buffer or one tablet with 15 ml. of buffer, depending of presentation.
The working reagent is stable 2 days refrigerated.
Calibration:
Use LABTROL serum calibrator.
Operation conditions:
Push on the key PARAMETER JOB and introduce the following information into the programs 1, 2, and 4,
and then continue following instructions of instrument.
1- TEST NAME
TEST [.....]
TEST NAME
AKP
2- CHEMISTRY PARAMETERS
TEST [.....]
ASSAY CODE 5 - 15 - 25
SAMPLE VOLUME (µl) 20
R1 VOLUME (µl) 300 - 20 - 0
R2 VOLUME (µl) 0 - 20 - 0
WAVELENGHT 660 - 415
CALIB. METHOD 2 - 0
STD 1 CONC. RACK POS. 0 - 1
UNIT
U/L
SD LIMIT 0.1
DUPLICATE LIMIT 100
SENSITIVITY 0
ABS. LIMIT 15000 - 0
PROZONE LIMIT 0
EXPECTED VALUE 0.0 - 5.4
INSTRUMENT FACTOR 1.00
3-CALIBRATION MONITOR
K=
4- CHANEL ASSIGNEMENT
PHASE [....]
TEST [....]
Linearity:
The method is linear until 150 U/L.
CHEMELEX, S.A. Polígono Industrial Can Castells. C/ Industria, 113 nave J 08420 Canovelles Tel/ +34 93 849 17 35
Fax/ +34 93 846 78 75 e-mail: chemelex@terra.es
Ed. 10.2002

LABKIT HITACHI 704
αAMYLASE
Preparation of working reagent:
Dissolve one tablet into one vial of buffer or one tablet with 15 ml. of buffer, depending of presentation.
The working reagent is stable 21 days refrigerated.
Calibration:
Use LABTROL serum calibrator.
Operation conditions:
Push on the key PARAMETER JOB and introduce the following information into the programs 1, 2, and 4,
and then continue following instructions of instrument.
1- TEST NAME
TEST [.....]
TEST NAME
AMIL
2- CHEMISTRY PARAMETERS
TEST [.....]
ASSAY CODE 5 - 5 - 10
SAMPLE VOLUME (µl) 10
R1 VOLUME (µl) 350 - [....] - 0
R2 VOLUME (µl) 0 - 20 - 0
WAVELENGHT 660 - 415
CALIB. METHOD 2 - 0
STD 1 CONC. RACK POS. 0 – 1
UNIT
U/L.
SD LIMIT 0.1
DUPLICATE LIMIT 100
SENSITIVITY 0
ABS. LIMIT 15000 - 0
PROZONE LIMIT 0
EXPECTED VALUE 0 - 90
INSTRUMENT FACTOR 1.00
3-CALIBRATION MONITOR
K=
4- CHANEL ASSIGNEMENT
PHASE [....]
TEST [....]
Linearity:
The method is linear until 1500 U/L.
CHEMELEX, S.A. Polígono Industrial Can Castells. C/ Industria, 113 nave J 08420 Canovelles Tel/ +34 93 849 17 35
Fax/ +34 93 846 78 75 e-mail: chemelex@terra.es
Ed. 10.2002

LABKIT HITACHI 704
URIC ACID
Preparation of working reagent:
Dissolve one vial of lyophilised R2 with a little quantity of buffer R1. When it's dissolved completely retune
to the original flack of buffer.
Calibration:
Use LABTROL serum calibrator.
Operation conditions:
Push on the key PARAMETER JOB and introduce the following information into the programs 1, 2, and 4,
and then continue following instructions of instrument.
1- TEST NAME
TEST [.....]
TEST NAME
URIC
2- CHEMISTRY PARAMETERS
TEST [.....]
ASSAY CODE 1 - 31 - 0
SAMPLE VOLUME (µ l) 10
R1 VOLUME (µl) 400 - [....] - 0
R2 VOLUME (µl) 0 – 1 – 0
WAVELENGHT 600 – 505
CALIB. METHOD 1 – 0
STD 1 CONC. RACK POS. 0.0 – 1
STD 2 CONC. RACK POS. [...] - 2
UNIT
mg/dl.
SD LIMIT 0.1
DUPLICATE LIMIT 100
SENSITIVITY 0
ABS. LIMIT 0 – 0
PROZONE LIMIT 0
EXPECTED VALUE 24 – 7.0
INSTRUMENT FACTOR 1.00
4- CHANEL ASSIGNEMENT
PHASE [....]
TEST [....]
Linearity:
The method is linear until 25 mg/dl.
CHEMELEX, S.A. Polígono Industrial Can Castells. C/ Industria, 113 nave J 08420 Canovelles Tel/ +34 93 849 17 35
Fax/ +34 93 846 78 75 e-mail: chemelex@terra.es
Ed. 10.2002

LABKIT HITACHI 704
CRP Turbi.
Preparation of working reagent:
Follow instructions given on the procedure.
Calibration:
Use CRP CALIBRATOR.
Operation conditions:
Linear Calibration.
1- TEST NAME
TEST [.....]
TEST NAME
CRP
2- CHEMISTRY PARAMETERS
TEST [.....]
ASSAY CODE [2 POINTS] - 20 - 32
SAMPLE VOLUME (µl) 3
R1 VOLUME (µl)
350 - 20 - NO
R2 VOLUME (µl)
50 - 20 - NO
WAVELENGHT 505
CALIB. METHOD NON-LINEAR 1 - 5
STD 1 CONC. RACK POS. 0.0 – 1
STD 2 CONC. RACK POS. 10 - 2
STD 3 CONC. RACK POS. 20 - 3
STD 4 CONC. RACK POS. 40 - 4
STD 5 CONC. RACK POS. 80 - 5
STD 6 CONC. RACK POS. 0.0 - 6
UNIT
mg/l.
SD LIMIT 300
DUPLICATE LIMIT 1000
SENSITIVITY 0
ABS. LIMIT
0 - INCREASE
PROZONE LIMIT
0 - LOWER
EXPECTED VALUE 0 - 8
INSTRUMENT FACTOR 1.0
CHEMELEX, S.A. Polígono Industrial Can Castells. C/ Industria, 113 nave J 08420 Canovelles Tel/ +34 93 849 17 35
Fax/ +34 93 846 78 75 e-mail: chemelex@terra.es
Ed. 10.2002

LABKIT HITACHI 704
ASO Turbi.
Preparation of working reagent:
Follow instructions given on the procedure.
Calibration:
Use ASO CALIBRATOR.
Operation conditions:
Non-Linear Calibration.
1- TEST NAME
TEST [.....]
TEST NAME
ASO
2- CHEMISTRY PARAMETERS
TEST [.....]
ASSAY CODE [2 POINTS] - 20 - 32
SAMPLE VOLUME (µl) 3
R1 VOLUME (µl)
350 - 20 - NO
R2 VOLUME (µl)
40 - 20 - NO
WAVELENGHT 505
CALIB. METHOD NON-LINEAR 1 - 5
STD 1 CONC. RACK POS. 0.0 – 1
STD 2 CONC. RACK POS. 100 - 2*
STD 3 CONC. RACK POS. 200 – 3
STD 4 CONC. RACK POS. 400 – 4
STD 5 CONC. RACK POS. 800 – 5
STD 6 CONC. RACK POS. 0.0 – 6
UNIT
IU/ml.
SD LIMIT 300
DUPLICATE LIMIT 1000
SENSITIVITY 0
ABS. LIMIT
0 - INCREASE
PROZONE LIMIT
0 - LOWER
EXPECTED VALUE 0 - 250
INSTRUMENT FACTOR 1.0
(*) Dilute standard ASO 1+1 with 0.9% NaCl saline solution to give a standard concentration of 400 IU/ml.
CHEMELEX, S.A. Polígono Industrial Can Castells. C/ Industria, 113 nave J 08420 Canovelles Tel/ +34 93 849 17 35
Fax/ +34 93 846 78 75 e-mail: chemelex@terra.es
Ed. 10.2002

LABKIT HITACHI 704
ASO Turbi.
Preparation of working reagent:
Follow instructions given on the procedure.
Calibration:
Use ASO CALIBRATOR.
Operation conditions:
Linear Calibration.
1- TEST NAME
TEST [.....]
TEST NAME
ASO
2- CHEMISTRY PARAMETERS
TEST [.....]
ASSAY CODE [2 POINTS] - 20 - 32
SAMPLE VOLUME (µl) 3
R1 VOLUME (µl)
350 - 20 - NO
R2 VOLUME (µl)
40 - 20 - NO
WAVELENGHT 505
CALIB. METHOD LINEAR 0 - 0
STD 1 CONC. RACK POS. 0.0 – 1
STD 2 CONC. RACK POS. 400 - 2*
STD 3 CONC. RACK POS. 0.0 – 3
STD 4 CONC. RACK POS. 0.0 – 4
STD 5 CONC. RACK POS. 0.0 – 5
STD 6 CONC. RACK POS. 0.0 – 6
UNIT
IU/ml.
SD LIMIT 300
DUPLICATE LIMIT 1000
SENSITIVITY 0
ABS. LIMIT
0 - INCREASE
PROZONE LIMIT
0 - LOWER
EXPECTED VALUE 0 - 250
INSTRUMENT FACTOR 1.0
(*) Dilute standard ASO 1+1 with 0.9% NaCl saline solution to give a standard concentration of 400 IU/ml.
CHEMELEX, S.A. Polígono Industrial Can Castells. C/ Industria, 113 nave J 08420 Canovelles Tel/ +34 93 849 17 35
Fax/ +34 93 846 78 75 e-mail: chemelex@terra.es
Ed. 10.2002

LABKIT HITACHI 704
CRP Turbi.
Preparation of working reagent:
Follow instructions given on the procedure.
Calibration:
Use CRP CALIBRATOR.
Operation conditions:
Non-Linear Calibration.
1- TEST NAME
TEST [.....]
TEST NAME
CRP
2- CHEMISTRY PARAMETERS
TEST [.....]
ASSAY CODE [2 POINTS] - 20 - 32
SAMPLE VOLUME (µl) 6
R1 VOLUME (µl) 350
R2 VOLUME (µl) 50
WAVELENGHT 505
CALIB. METHOD NON-LINEAR 1 - 5
STD 1 CONC. RACK POS. 0.0 – 1
STD 2 CONC. RACK POS. 5 - 2
STD 3 CONC. RACK POS. 10 - 3
STD 4 CONC. RACK POS. 20 - 4
STD 5 CONC. RACK POS. 40 - 5
STD 6 CONC. RACK POS. 0.0 - 6
UNIT
mg/l.
SD LIMIT 300
DUPLICATE LIMIT 1000
SENSITIVITY 0
ABS. LIMIT
0 - INCREASE
PROZONE LIMIT
0 - LOWER
EXPECTED VALUE 0 - 8
INSTRUMENT FACTOR 1.0
#Enhanced sensitivity (i.e. paediatric samples).
CHEMELEX, S.A. Polígono Industrial Can Castells. C/ Industria, 113 nave J 08420 Canovelles Tel/ +34 93 849 17 35
Fax/ +34 93 846 78 75 e-mail: chemelex@terra.es
Ed. 10.2002

LABKIT HITACHI 704
RF Turbi.
Preparation of working reagent:
Follow instructions given on the procedure.
Calibration:
Use RF CALIBRATOR.
Operation conditions:
Non-Linear Calibration.
1- TEST NAME
TEST [.....]
TEST NAME
RF
2- CHEMISTRY PARAMETERS
TEST [.....]
ASSAY CODE [2 POINTS] - 20 - 32
SAMPLE VOLUME (µl) 3
R1 VOLUME (µl)
350 - 20 - NO
R2 VOLUME (µl)
40 - 20 - NO
WAVELENGHT 600
CALIB. METHOD NON-LINEAR 1 - 5
STD 1 CONC. RACK POS. 0.0 – 1
STD 2 CONC. RACK POS. 16.5 – 2*
STD 3 CONC. RACK POS. 33 – 3*
STD 4 CONC. RACK POS. 66 – 4*
STD 5 CONC. RACK POS. 132 – 5*
STD 6 CONC. RACK POS. 0.0 – 6
UNIT
IU/ml.
SD LIMIT 300
DUPLICATE LIMIT 1000
SENSITIVITY 0
ABS. LIMIT
0 - INCREASE
PROZONE LIMIT
0 - LOWER
EXPECTED VALUE 0 - 20
INSTRUMENT FACTOR 1.0
*Depends on the concentration of calibrated used.
CHEMELEX, S.A. Polígono Industrial Can Castells. C/ Industria, 113 nave J 08420 Canovelles Tel/ +34 93 849 17 35
Fax/ +34 93 846 78 75 e-mail: chemelex@terra.es
Ed. 10.2002