D. MONOGRAPHS Calcium Pantothenate

D. MONOGRAPHS
Calcium Pantothenate
C18H32CaN2O10 Mol. Wt. 476.54
monocalcium bis(R)-N-(2,4-dihydroxy-3,3-dimethylbutyryl)-3-aminopropanoate
137-08-6
Content Calcium Pantothenate, when calculated on the dried basis, contains
5.76.0% of nitrogen (N=14.01) and 8.28.6% of calcium (Ca=40.08).
Description Calcium Pantothenate occurs as a white powder. It is odorless and
has a slightly bitter taste.
Identification (1) Dissolve 50 mg of Calcium Pantothenate in 5 ml of sodium
hydroxide solution (1 25), and add 1 drop of cupric sulfate solution (1 10). A
blue-purple color develops.
(2) To 50 mg of Calcium Pantothenate, add 5 ml of sodium hydroxide solution (1
25), and boil for 1 minute. Cool, and add 2 ml of diluted hydrochloric acid (1 3)
and 2 drops of ferric chloride solution (1 10). A dark yellow color develops.
(3) Calcium Pantothenate solution (1 20) responds to all tests for Calcium
Salt as described in the Qualitative Tests.
Purity (1) Specific rotation [ α ] 20
D
: +25.0+28.5(after dried, 1.25 g, water,
25 ml).
(2) pH 7.09.0.
Test Solution Weigh 2.0 g of Calcium Pantothenate, and add water to make 10
ml.
(3) Heavy metals Not more than 20µg/g as Pb (1.0 g, Method 1, Control
Solution Lead Standard Solution 2.0 ml).
(4) Arsenic Not more than 4.0µg/g as As2O3 (0.50 g, Method 1, Apparatus B).
(5) Alkaloid Weigh 50 mg of Calcium Pantothenate, dissolve in 5 ml of water,
and add 0.5 ml of ammonium molybdate TS and 0.5 ml of phosphoric acid (1 10).
No white turbidity appears.
Loss on Drying Not more than 5.0% (105, 3 hours).
Assay (1) Nitrogen Weigh accurately about 50 mg of Calcium Pantothenate,
proceed as directed in the Semi micro Kjeldahl Method under Nitrogen
Determination, and calculate on the dried basis.

D. MONOGRAPHS
(2) Calcium Weigh accurately about 2.5 g of Calcium Pantothenate, dissolve in
5 ml of diluted hydrochloric acid (1 4) and 20 ml of water, and add water to make
exactly 50 ml. Proceed as directed in Method 1 under Calcium Salt Determination,
using this solution as the test solution, and calculate on the dried basis.
1 ml of 0.05 mol/l EDTA = 2.0039 mg of Ca