CST Guide:

Section I: Research Areas
chapter 03: Cell Growth and Death
These protein targets represent key
nodes within apoptotic signaling
pathways and are commonly studied
in apoptosis research. Primary
antibodies, antibody conjugates, and
antibody sampler kits containing
these targets are available from CST.
Listing as of September 2014. See our
website for current product information.
M Monoclonal Antibody
P Polyclonal Antibody
E PathScan ® ELISA Kits
S SignalSilence ® siRNA
C Antibody Conjugate
Inducers of Apoptosis
Stimulating apoptosis with chemical modulators or cytokines is a common strategy for investigating effects on downstream
signaling. These agents induce apoptosis by a variety of methods, typically through inhibition of key cellular processes or kinases.
#9972 Brefeldin A Interferes with ER to Golgi transport; typically used to study vesicle trafficking but
prolonged exposure causes apoptosis
#2112 Cycloheximide Inhibits protein synthesis and is often used in conjunction with TNF-α to induce cell death
#2200 Etoposide Inhibits Topoisomerase II
#9807 Paclitaxel Binds β-tubulin and prevents microtubule depolymerization
#9885 Roscovitine Inhibits CDK1, CDK2, CDK5 to cause cell cycle arrest and apoptosis
#9953 Staurosporine Potently inhibits PKC; also inhibits PKA, PKG, CAMKII, and MLCK
#5927 Doxorubicin Inhibits DNA and RNA synthesis through intercalating the DNA double helix
#8902 Human Tumor Necrosis
Factor-α (hTNF-α)
#5178 Mouse Tumor Necrosis
Factor-α (mTNF-α)
#4698 Mouse His 6 Tumor Necrosis
Factor-α (m His 6TNF-α)
Commonly Studied Apoptosis Targets
Target M P E S C
A1/Bfl-1
•
APR3
•
Acinus
•
AIF • • •
Alix
•
AP-2α
• •
AP-2β
•
AP-2g
•
Phospho-AP2M1 (Thr156) •
Apaf-1 • • •
Aven
•
Bad
• • • •
Phospho-Bad (Ser112) • • • •
Phospho-Bad (Ser136) • •
Phospho-Bad (Ser155) • •
Bak1 • • •
BAP31
•
Bax • • •
Bcl-2
• • • •
Phospho-Bcl-2 (Thr56) •
Phospho-Bcl-2 (Ser70) • • •
Bcl-w
•
Bcl-xL • • • •
BCL2L10
•
A1/Bfl-1
•
BID
• •
Bik
•
Bim • • • •
Phospho-Bim (Ser55)
•
Phospho-Bim (Ser69) • •
Phospho-Bim (Ser77) •
BIRC6
•
Bit1
•
Bmf
•
c-IAP1
• •
Triggers extrinsic apoptosis and/or survival through death receptor signaling
Triggers extrinsic apoptosis and/or survival through death receptor signaling
Triggers extrinsic apoptosis and/or survival through death receptor signaling
#5452 Fas Ligand Triggers extrinsic apoptosis through death receptor signaling
Target M P E S C
c-IAP2
•
Caspase Cleavage Motif •
Caspase-1
•
Cleaved Caspase-1 (Asp297) •
Caspase-2
•
Caspase-3 • • •
Cleaved Caspase-3 (Asp175) • • • •
Caspase-4
•
Caspase-5
•
Caspase-6
•
Cleaved Caspase-6 (Asp162) •
Caspase-7
• •
Cleaved Caspase-7 (Asp198) • •
Caspase-8
•
Cleaved Caspase-8 (Asp384) •
Cleaved Caspase-8 (Asp387) • •
Cleaved Caspase-8 (Asp391) • •
Caspase-9
• •
Cleaved Caspase-9 (Asp315) •
Cleaved Caspase-9 (Asp330) • •
Cleaved Caspase-9 (Asp353) •
Non-Phospho-Caspase-9 (Ser196)
•
Caspase-11
•
Caspase-12
•
Caspase-14
•
Cytochrome c • • •
DAP1
•
DAPK1
•
DAPK3/ZIPK
•
Daxx
•
Cleaved Drosophila Dcp-1 (Asp216) •
DcR1
•
DcR2
•
DcR3
•
DFF45/DFF35
•
Target M P E S C
Cleaved DFF45 (Asp224) •
DIDO1
•
DR3
•
DR5
• •
Phospho-DR6 (Ser562) •
DRAK2
•
eIF4G2/p97
•
Endonuclease G
•
FADD
•
Phospho-FADD (Ser194) •
FAF1
•
FAIM
•
Fas
•
Fas Ligand
•
FLIP • •
α-Fodrin
•
Cleaved α-Fodrin (Asp1185) •
Granzyme A
•
Granzyme B
•
HIPK2
•
HtrA2/Omi • • •
Cleaved Drosophila ICE (drICE)
(Asp230)
•
Lamin A/C • • •
Cleaved Lamin A/C (Small Subunit) • •
Phospho-Lamin A/C (Ser22) • • •
Lamin B1
•
Lamin B2
•
LAP2α
•
Livin
•
Mad-1
•
Maspin
•
Max
•
Mcl-1 • • •
Phospho-Mcl-1 (Ser64) •
Phospho-Mcl-1 (Ser159/Thr163) •
Phospho-Mcl-1 (Thr183)/
MST2(Thr180)
Phospho-MLKL
Mst1
•
•
•
Target M P E S C
Mst2
•
Mst3
•
Mst4
•
c-Myc • • •
Phospho-c-Myc (Ser62) •
N-Myc
•
PAR-4
•
Phospho-PAR-4 (Thr163) •
PARP1 • •
Cleaved-PARP1 (Asp214) • • • •
PDCD4
•
PEA-15 • • •
Phospho-PEA-15 (Ser104) •
Perforin
•
PHLDA3
•
Puma • • •
RIP1
• •
RIP3
• •
Siva-1
•
Smac/Diablo
•
Survivin
• • • • •
Phospho-Survivin (Thr34) • •
TANK
•
TAX1BP1
•
TMS1
•
TP/ECGF1
• •
TNF-R2
•
TRADD
• •
TRAF1
•
TRAF2
•
Phospho-TRAF2 (Ser11) •
TRAF3
•
TRAF6
•
TRAIL
•
VDAC1
• •
VDAC2
•
WWOX
•
XAF1
• •
XIAP • • •
1,240
2012–2014 citations
CST antibodies for cleaved caspase-3
(Asp175) have been cited over 1,240
times in high-impact, peer-reviewed
publications from the global research
community.
Select Citations:
Bjordal, M. et al. (2014) Sensing
of amino acids in a dopaminergic
circuitry promotes rejection of an
incomplete diet in Drosophila. Cell
156, 510–521.
Doitsh, G. et al. (2014) Cell death by
pyroptosis drives CD4 T-cell depletion
in HIV-1 infection. Nature 505,
509–514.
Walczynski, J. et al. (2014) Sensitisation
of c-MYC-induced B-lymphoma
cells to apoptosis by ATF2. Oncogene
33, 1027–1036.
de Poot, S.A. et al. (2014) Granzyme
M targets topoisomerase II alpha to
trigger cell cycle arrest and caspasedependent
apoptosis. Cell Death
Differ. 21, 416–426.
Pencheva, N. et al. (2014) Broadspectrum
therapeutic suppression of
metastatic melanoma through nuclear
hormone receptor activation. Cell
156, 986–1001.
Ardestani, A. et al. (2014) MST1 is a
key regulator of beta cell apoptosis
and dysfunction in diabetes. Nat.
Med. 20, 385–397.
von Figura, G. et al. (2014) The
chromatin regulator Brg1 suppresses
formation of intraductal papillary
mucinous neoplasm and pancreatic
ductal adenocarcinoma. Nat. Cell Biol.
16, 255–267.
Beug, S.T. et al. (2014) Smac
mimetics and innate immune stimuli
synergize to promote tumor death.
Nat. Biotechnol. 32, 182–190.
Gao, T. et al. (2014) Pdx1 maintains
beta cell identity and function by
repressing an alpha cell program. Cell
Metab. 259–271.
Chen, Y. et al. (2014) Hyperactivation
of mammalian target of rapamycin
complex 1 (mTORC1) promotes
breast cancer progression through
enhancing glucose starvation-induced
autophagy and Akt signaling. J. Biol.
Chem. 289, 1164–1173.
Razorenova, O.V. et al. (2014) The
apoptosis repressor with a CARD domain
(ARC) gene is a direct hypoxiainducible
factor 1 target gene and
promotes survival and proliferation of
VHL-deficient renal cancer cells. Mol.
Cell. Biol. 34, 739–751.
88 For Research Use Only. Not For Use in Diagnostic Procedures. See pages 302 & 303 for Pathway Diagrams, Application, and Reactivity keys.
www.cellsignal.com/cstapoptosis 89