CST Guide:

Section I: Research Areas
chapter 02: Signaling
These protein targets represent key
nodes within MAP Kinase pathways
and are commonly studied in MAP
Kinase research. Primary antibodies,
antibody conjugates, and antibody
sampler kits containing these targets
are available from CST.
Listing as of September 2014. See our
website for current product information.
M Monoclonal Antibody
P Polyclonal Antibody
E PathScan ® ELISA Kits
S SignalSilence ® siRNA
C Antibody Conjugate
Chemical Modulators for the Study of MAP Kinase Signaling
#2222 Anisomycin Inhibits protein synthesis; activates stress-activated kinases (SAPK/JNK, p38-MAPK)
#11916 Chelerythrine
Chloride
SP600125 is a highly specific inhibitor of JNK-family kinases.
SP600125 #8177: WB analysis of extracts from 293T cells, untreated or
treated with Anisomycin #2222 (25 μg/ml) for the indicated times either with
or without #8177 pre-treatment (50 μM, 40 min), using Phospho-JunB
(Thr102/Thr104) (D3C6) Rabbit mAb #8053 (upper) or JunB (C37F9) Rabbit
mAb #3753 (lower).
Commonly Studied MAP Kinase Targets
Target M P E S C
ASK1
• •
Phospho-ASK1 (Ser83) •
Phospho-ASK1 (Thr845) •
Phospho-ASK1 (Ser967) •
APS
•
β-Arrestin 1 • •
Phospho-β-Arrestin 1 (Ser412) •
β-Arrestin 1/2
•
β-Arrestin 2
•
ATF-2 • •
Phospho-ATF-2 (Thr69/Thr71) •
Phospho-ATF-2 (Thr71) • • • •
Bcr
•
Phospho-Bcr (Tyr177)
•
Bcr-Abl (b2a2 Junction Specific) •
c-Abl
•
Phospho-c-Abl (Tyr89) •
Phospho-c-Abl (Tyr204) •
Phospho-c-Abl (Tyr245) • •
Phospho-c-Abl (Tyr412) • •
Phospho-c-Abl (Thr735) •
Csk
•
Dexras1
Dok1
Cell-permeable inhibitor of PKC; activates SAPK/JNK and p38-MAPK;
Induces apoptosis in some cell lines
#9900 PD98059 Highly selective inhibitor of MEK1 and MEK2; binds
inactive forms and prevent activation by upstream kinases
#12147 PD184352 Highly selective, noncompetitive inhibitor of MEK1 and MEK2
#8158 SB202190 Cell-permeable, highly specific inhibitor of p38-MAPK (ATP-competitive inhibitor)
#5633 SB203580 Cell-permeable inhibitor of p38-MAPK (inhibits PDK1 at higher concentrations)
#8705 Sorafenib Inhibits VEGFR and PDGFR; inhibits Raf kinases; induces autophagy
#8177 SP600125 Cell-permeable, highly specific inhibitor of JNK-family kinases
#9903 U0126 Highly selective inhibitor of MEK1 and MEK2
•
•
kDa
140
100
80
60
50
40
30
20
140
100
80
60
50
40
30
20
Phospho-JunB
(Thr102/Thr104)
JunB
0 15 30 0 15 30 Anisomycin (min)
– – – + + + SP600125
Target M P E S C
Dok2
•
Phospho-Dok2 (Tyr345) •
Phospho-DUSP1 (Ser359) •
DUSP3/VHR
•
DUSP4/MKP2
•
DUSP6/MKP3
•
DUSP10/MKP5
•
DUSP16/MKP7
•
Elk-1
•
Phospho-Elk-1 (Ser383) • •
Erk1 (p44 MAPK) • •
Erk1/2 (p44/42 MAPK) • • • •
Phospho-Erk1/2 (Thr202/Tyr204) • • • •
Erk2 (p42 MAPK) • •
Erk5 • • •
Phospho-Erk5 (Thr218/Tyr220) •
FAM129B
•
c-Fos • • •
Phospho-c-Fos (Ser32) • •
FosB • • •
FRA1
•
Phospho-FRA1 (Ser265) • • •
Phospho-FRS2-α (Tyr196) •
Phospho-FRS2-α (Tyr436) •
Target M P E S C
GCK
•
Grb2 • •
Grb10
•
HGK
• •
JIP4/SPAG9
•
JNK1/SAPK
• • • •
Phospho-JNK1/SAPK (Thr183/Tyr185) • • • •
JNK2
•
JNK3
•
c-Jun • •
Phospho-c-Jun (Ser63) • • •
Phospho-c-Jun (Ser73) • • •
Phospho-c-Jun (Thr91) •
Phospho-c-Jun (Thr93) •
Phospho-c-Jun (Ser243) •
JunB
• •
JunD
•
KSR1
•
Phospho-KSR1 (Ser392) •
MAPKAPK-2
• •
Phospho-MAPKAPK-2 (Thr222) •
Phospho-MAPKAPK-2 (Thr334) • • •
MAPKAPK-3
• •
MAPKAPK-5
•
MEF2A
•
Phospho-MEF2A (Ser408) •
MEF2C
•
MEK1 • • • •
Phospho-MEK1 (Thr286) •
Phospho-MEK1 (Ser298) •
MEK1/2 • • •
Phospho-MEK1/2 (Ser217/221) • • • •
MEK2 • • •
MEKK3
•
Mig6
•
MKK3 • • •
• •
Phospho-MKK3 (Ser189)/MKK6
(Ser207)
SEK1/MKK4
• •
Phospho-SEK1/MKK4 (Ser80) •
Phospho-SEK1/MKK4 (Ser257) •
Phospho-SEK1/MKK4 (Ser257/Thr261) •
Phospho-SEK1/MKK4 (Thr261) •
MKK6
• •
MKK7 • •
Phospho-MKK7 (Ser271/Thr275) •
MLK1
•
MLK3
•
MSK1
•
Phospho-MSK1 (Ser360) •
Phospho-MSK1 (Ser376) •
Phospho-MSK1 (Thr581) •
MSK2
•
p38 MAPK • • • •
Phospho-p38 MAPK (Thr180/Tyr182) • • • •
p38-α MAPK • • •
p38-β MAPK • •
Target M P E S C
p38-δ MAPK • • •
p38-γ MAPK • •
PP2C α
•
Phospho-PTPα (Tyr798) •
Pyk2 • • •
Phospho-Pyk2 (Tyr402) • •
A-Raf
•
Phospho-A-Raf (Ser299) •
B-Raf • •
Phospho-B-Raf (Ser445) •
c-Raf • • •
Phospho-c-Raf (Ser259) •
Phospho-c-Raf
(Ser289/Ser296/Ser301)
Phospho-c-Raf (Ser296)
Phospho-c-Raf (Ser338)
Ras
•
•
• •
• •
RKIP • • •
p90RSK1
• • • •
Phospho-p90RSK (Thr359) •
Phospho-p90RSK (Thr359/Ser363) •
Phospho-p90RSK (Thr573) •
Phospho-p90RSK (Ser380) • • • •
RSK1/RSK2/RSK3
• •
RSK2
• •
Phospho-RSK2 (Ser227) •
RSK3
•
Phospho-RSK3 (Thr356/Ser360) •
Shc
•
Phospho-Shc (Tyr239/240) •
Phospho-Shc (Tyr317)
•
Phospho-SHIP2 (Tyr1135) •
SHP-2 • • •
Phospho-SHP-2 (Tyr542) •
Phospho-SHP-2 (Tyr580) • • •
SOS1
• •
Src • • • •
Phospho-Src (Ser17) • •
Phospho-Src (Tyr416)
•
Phospho-Src (Tyr527)
•
Phospho-Src Family (Tyr416) • • •
Non-phospho-Src (Tyr416) •
Non-phospho-Src (Tyr527) •
SRF
•
Phospho-SRF (Ser103) •
TAB1
•
TAB2
• •
Phospho-TAB2 (Ser372) •
TAK1 • • •
Phospho-TAK1 (Thr184) •
Phospho-TAK1 (Thr184/Thr187) • •
Phospho-TAK1 (Thr187)
Phospho-TAK1(Ser412)
Tnk1
Phospho-Tnk1 (Tyr277)
Phospho-Tpl2/Cot (Ser400)
•
•
•
• •
•
745
2012–2014 citations
CST antibodies for Phospho-Erk1/2
(Thr202/Try204) have been cited
over 745 times in high-impact, peerreviewed
publications from the global
research community.
Select Citations:
Kusumbe, A.P. et al. (2014) Coupling
of angiogenesis and osteogenesis
by a specific vessel subtype in bone.
Nature 507, 323–328.
Brady, D.C. et al. (2014) Copper is
required for oncogenic BRAF signalling
and tumorigenesis. Nature 509,
492–496.
Dhandapany, P.S. et al. (2014) RAF1
mutations in childhood-onset dilated
cardiomyopathy. Nat. Genet. 46,
635–639.
Ota, K.T. et al. (2014) REDD1 is
essential for stress-induced synaptic
loss and depressive behavior. Nat.
Med. 20, 531–535.
von Figura, G. et al. (2014) The
chromatin regulator Brg1 suppresses
formation of intraductal papillary
mucinous neoplasm and pancreatic
ductal adenocarcinoma. Nat. Cell Biol.
16, 255–267.
Paul, S. et al. (2014) T cell receptor
signals to NF-kappaB are transmitted
by a cytosolic p62-Bcl10-Malt1-IKK
signalosome. Sci. Signal. 7, ra45.
Barcelo, C. et al. (2014) Phosphorylation
at Ser-181 of oncogenic KRAS
is required for tumor growth. Cancer
Res. 74, 1190–1199.
Buonato, J.M. et al. (2014) ERK1/2
blockade prevents epithelial-mesenchymal
transition in lung cancer cells
and promotes their sensitivity to EGFR
inhibition. Cancer Res. 74, 309–319.
Cordero, J.B. et al. (2014) c-Src
drives intestinal regeneration
and transformation. EMBO J. 33,
1474–1491.
Kabekkodu, S.P. et al. (2014) DNA
promoter methylation-dependent
transcription of the double C2-like
domain beta (DOC2B) gene
regulates tumor growth in human
cervical cancer. J. Biol. Chem. 289,
10637–10649.
44 For Research Use Only. Not For Use in Diagnostic Procedures. See pages 302 & 303 for Pathway Diagrams, Application, and Reactivity keys.
www.cellsignal.com/cstmapk 45