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31<br />

Section IV: Additional Information<br />

Index<br />

by Pathways, Protocols, and targets<br />

Pathway Diagrams and Tables<br />

Adherens Junction Dynamics 110<br />

Akt Binding Partners 65<br />

Akt Substrates 55<br />

AMPK Signaling 146<br />

AMPK Substrates 147<br />

Amyloid Plaque and Neurofibrillary Tangle 190<br />

Formation in Alzheimer’s Disease<br />

Angiogenesis Signaling in<br />

171<br />

Tumor Neovascularization<br />

Autophagy Signaling 97<br />

B Cell Receptor Signaling 178<br />

Calcium, cAMP, and Lipid Signaling<br />

84<br />

Kinase-Disease Associations<br />

Cell Cycle Control: G1/S Checkpoint 104<br />

Cell Cycle Control: G2/M DNA Damage 105<br />

Checkpoint<br />

Cell Cycle/Checkpoint Control<br />

102<br />

Kinase-Disease Associations<br />

Death Receptor Signaling 92<br />

Deubiquitinase Table 135<br />

Dopamine Signaling in Parkinson’s Disease 191<br />

ErbB/HER Signaling 74<br />

ESC Pluripotency Differentiation 165<br />

Examples of Crosstalk Between<br />

31<br />

Post-Translational Modifications<br />

G-Protein Coupled Receptor<br />

79<br />

Signaling to MAP Kinase/Erk<br />

G-Protein Coupled Receptor Signaling: Overview 78<br />

Hedgehog Signaling 163<br />

Hippo Signaling 162<br />

Histone Lysine Methylation 27<br />

Histone Modifications Table 28<br />

Inhibition of Apoptosis 91<br />

Protocols and Troubleshooting <strong>Guide</strong>s<br />

ChIP General Protocol 196<br />

ChIP Troubleshooting <strong>Guide</strong> 200<br />

Cultured Cells (Immunocytochemistry, IF-IC) 210<br />

Protocol<br />

Flow Cytometry Alternate Protocol 204<br />

Flow Cytometry General Protocol 204<br />

Frozen/Cryostat Tissue Sections (IF-F) Protocol 211<br />

IHC Frozen Protocol<br />

217<br />

(using SignalStain ® Boost Detection Reagent)<br />

IHC Paraffin Protocol<br />

216<br />

(using SignalStain ® Boost Detection Reagent)<br />

IHC Troubleshooting <strong>Guide</strong> 219<br />

Insulin Receptor Signaling 144<br />

Jak and Cytokine Receptor Mutants 176<br />

Jak/Stat Signaling: IL-6 Receptor Family 181<br />

Jak/Stat Utilization 177<br />

MAPK/Erk in Growth and Differentiation 46<br />

Mitochondrial Control of Apoptosis 93<br />

mTOR Signaling 54<br />

NF-κB Signaling 182<br />

Notch Signaling 161<br />

Nuclear Receptors Signaling 41<br />

PI3 Kinase/Akt Signaling 53<br />

Protein Acetylation 26<br />

Regulation of Actin Dynamics 115<br />

Regulation of Apoptosis Overview 90<br />

Regulation of Microtubule Dynamics 116<br />

SAPK/JNK Signaling 48<br />

Signaling Pathways Activating p38 MAP Kinase 47<br />

T Cell Receptor Signaling 179<br />

TGF-β Signaling 164<br />

Toll-like Receptor Signaling 180<br />

Translational Control: Overview 36<br />

Translational Control: Regulation of elF2 38<br />

Translational Control: Regulation of elF4E 37<br />

and p70 S6K<br />

Tumor Immunology 183<br />

Tyrosine Kinases Kinase-Disease Associations 71<br />

Ubiquitin Ligases 125<br />

Ubiquitin/Proteasome 124<br />

Vesicle Trafficking in Presynaptic Neurons: 189<br />

Synchronous Release<br />

Warburg Effect 145<br />

Wnt/β-Catenin Signaling 160<br />

In-Cell Western Protocol 213<br />

IP Denatured Protein Protocol 237<br />

IP Native Protein Protocol 235<br />

Paraffin Tissue Sections (IF-P) Protocol 212<br />

PathScan ® Sandwich ELISA<br />

224<br />

Antibody Pair Protocol<br />

PathScan ® Sandwich ELISA<br />

223<br />

Chemiluminescent Protocol<br />

PathScan ® Sandwich ELISA Colorimetric Protocol 221<br />

WB Fluorescent Protocol 230<br />

WB General Protocol 228<br />

WB Troubleshooting <strong>Guide</strong> 232<br />

Targets<br />

14-3-3 113<br />

4E-BP 35<br />

4EHP 35<br />

4E-T 35<br />

4F2hc/CD98 142<br />

5-HTR1A 187<br />

5-HTR4 187<br />

53BP1 101<br />

A1/Bfl-1 88<br />

A20/TNFAIP3 174<br />

A2B5 187<br />

ABCC4 142<br />

ABCG2 142<br />

ABIN-1 174<br />

c-Abl 44<br />

ACAD 142<br />

ACAT2 142<br />

ACE2 119<br />

AceCS1 142<br />

Acetyl-CoA Carboxylase 142<br />

ACF1 24<br />

Acinus 88<br />

Ack1 113<br />

Actin 113<br />

ACO2 142<br />

ACPP 69<br />

ACSL1 142<br />

α-Actinin 113<br />

ACVR1 158<br />

ADAM 109<br />

ADAMTS1 109, 170<br />

ADAP 174<br />

ADAR1 35<br />

ADH1 142<br />

Adiponectin 142<br />

β-Adrenergic Receptor 77, 83<br />

ADRM1 123<br />

Afadin 109, 113<br />

AFP 158<br />

AGR2 158<br />

AhR 39<br />

AID 174<br />

AIF 88<br />

AIM2 174<br />

Aiolos 174<br />

Ajuba 109<br />

AKAP 77, 83<br />

AKR1C2 142<br />

Akt 51<br />

ALDH1A1 142<br />

Aldolase A 142<br />

Alix 88<br />

ALK 69<br />

ALPP 158<br />

AMACR 142<br />

Ambra1 96<br />

AMFR 123<br />

AML1 174<br />

AMPA Receptor 187<br />

AMPK 142<br />

Amylase 142<br />

β-Amyloid 187<br />

Androgen Receptor 39<br />

Angiopoietin-2 158, 170<br />

Annexin 83, 113<br />

ANT2/SLC25A5 142<br />

AP-2 88<br />

AP2M1 88, 113<br />

Apaf-1 88<br />

APBA2 187<br />

APC 100, 123, 158<br />

Ape1 100<br />

ApoA 83<br />

ApoE 187<br />

ApoM 83<br />

APP/β-Amyloid 187<br />

APPL1 119<br />

APS 44<br />

AQP2 142<br />

Arf6 119<br />

Arginase-1 142<br />

Argonaute 35<br />

ARID1A/BAF250A 24<br />

ARK5 142<br />

Aromatase 39<br />

ARP 113<br />

Arrestin 44, 77, 187<br />

Artemis 100<br />

AS160 142<br />

ASCT2 142<br />

ASF1 24<br />

ASH2L 24<br />

ASK1 44<br />

ASM 83<br />

Ataxin-1 187<br />

ATF 44, 142<br />

Atg 96<br />

ATGL 142<br />

Atlastin-1 113<br />

ATM 100<br />

ATP Citrate Lyase 142<br />

ATP2A/SERCA 83<br />

ATP6V1B1/2 51<br />

ATPIF1 142<br />

ATR 100<br />

ATRIP 100<br />

AUF1/hnRNP D 35<br />

Aurora 100<br />

Aven 88<br />

Axin 158<br />

Axl 69<br />

BACE 187<br />

BACH 100, 174<br />

Bad 88<br />

BAFF 174<br />

Bag1 119<br />

BAG6 119<br />

Bak1 88<br />

BAP1 123<br />

BAP31 88<br />

Basigin/EMMPRIN 174<br />

Bassoon 187<br />

BATF 174<br />

Bax 88<br />

BCAT 142<br />

Bcl-2 88<br />

BCL2L10 88<br />

BCL6 174<br />

Bcl10 174<br />

Bcl-11B 158<br />

Bcl-w 88<br />

Bcl-xL 88<br />

Bcr 44<br />

Bcr-Abl 44<br />

Beclin-1 96<br />

BID 88<br />

Bif-1 96<br />

Bik 88<br />

Bim 88<br />

BiP 119<br />

BIRC6 88<br />

Bit1 88<br />

Blimp-1/PRDI-BF1 174<br />

Blk 174<br />

BLM 100<br />

BLNK 174<br />

BMAL 187<br />

Bmf 88<br />

Bmi1 24<br />

BMP 158<br />

BMPR2 158<br />

BNIP3 96<br />

BNIP3L/Nix 96<br />

Bora 100<br />

BORIS 24<br />

Brachyury 158<br />

BRCA1 100<br />

BRD 24<br />

BrdU 100<br />

chapter 31: Index<br />

BRE 100<br />

BRF1/2 35<br />

Brg1 24<br />

BRM 24<br />

Brn2/POU3F2 187<br />

BRSK 187<br />

BSP II 109<br />

BTAF1 24<br />

Btk 174<br />

Bub 100<br />

C1QBP 142<br />

CA 142, 170<br />

CABIN1 24<br />

CACYBP 158<br />

CAD 142<br />

Cadherin 109, 170<br />

Calbindin 187<br />

Calcineurin A 83<br />

Caldesmon-1 113<br />

Calmodulin 83<br />

Calnexin 119<br />

Calpastatin 119<br />

Calumenin 83<br />

CaMKI 187<br />

CAND1 123<br />

CARD 174<br />

CASK 187<br />

Caspases 88<br />

Caspr2 187<br />

Catalase 142<br />

Catenin 109, 113, 158<br />

Cathepsin B 187<br />

Caveolin-1 113, 119<br />

CBARA1/MICU1 83<br />

CBFβ 174<br />

Cbl-b 123<br />

c-Cbl 123<br />

CBP 24, 170<br />

CCR2 174<br />

CCT2 119<br />

CCTα 142<br />

CD (Cell Surface<br />

Markers)<br />

109, 113, 170,<br />

174, 187<br />

CD2AP 113<br />

Cdc2 100<br />

Cdc6 100<br />

Cdc7 100<br />

CDC20 100<br />

cdc25 100<br />

CDC37 100, 113, 119<br />

Cdc42 113<br />

Cdc45 100<br />

CDC73 100<br />

294 For Research Use Only. Not For Use in Diagnostic Procedures.<br />

295

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