CST Guide:
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27<br />
Section III: Workflow Tools<br />
Customization<br />
antibodies in a carrier-free<br />
formulation for specific assay platforms<br />
How can I obtain an antibody formulated for<br />
the special requirements of my assay platform<br />
Researchers studying protein activities and associations can choose from a wide array of high throughput<br />
assay platforms. Many of these require antibodies in specific concentrations or in formulations free<br />
of BSA or other carrier proteins that are typically found in off-the-shelf antibody storage buffers. Custom<br />
antibody formulations from Cell Signaling Technology (<strong>CST</strong>) are highly specific, in-house validated<br />
antibodies that are ready to be labeled or used directly in your assay platform, providing the flexibility<br />
necessary for your unique experiment.<br />
Single peak indicates the antibody is intact and shows no cleavage or degradation.<br />
AU<br />
0.070<br />
0.065<br />
0.060<br />
0.055<br />
0.050<br />
0.045<br />
0.040<br />
0.035<br />
0.030<br />
0.025<br />
0.020<br />
Single peak indicates that the<br />
antibody is intact and shows<br />
no cleavage or degradation.<br />
chapter 27: Customization<br />
PD-L1 (E1L3N ® ) XP ® Rabbit mAb<br />
#13684: Size exclusion chromatogram<br />
of #13684 formulated in PBS buffer.<br />
Custom formulated antibodies can be used to:<br />
• Label antibodies with fluorochromes, lanthanides, or molecules for use on specific platforms such<br />
as AlphaScreen ® , DELFIA ® or HTRF assays<br />
• Develop pair-based sandwich assays for use on specific platforms such as Bio-Plex ® or Luminex ®<br />
• Develop custom plate-based sandwich ELISA kits<br />
• Design custom multiplex assays where several protein readouts are assayed using multiple targetspecific<br />
antibodies<br />
0.015<br />
0.010<br />
0.005<br />
0.000<br />
2 4 6 8 10 12 14 16 18 20 22 24 26 28 30<br />
minutes<br />
<strong>CST</strong> can supply antibodies in phosphate buffered saline (PBS) or borate buffer at your specified concentration.<br />
Once the antibodies are formulated in the specified buffer, we validate them for molecular integrity<br />
by size exclusion chromatography and application-specific functionality using WB, IP, peptide ELISA,<br />
flow cytometry, IHC, or IF. The application testing is dependent on the proposed use of the antibody.<br />
Peptide ELISA confirms phospho-specificity of multiple antibody lots.<br />
How custom formulated antibodies<br />
from <strong>CST</strong> can benefit your research:<br />
• <strong>CST</strong> is ISO9001:2008 certified for the production and supply of monoclonal antibodies, ensuring<br />
reproducible, traceable product quality<br />
• Antibodies are supplied in formulations adaptable to multiple platforms<br />
• Each antibody is custom manufactured with complete documentation support to ensure<br />
product integrity<br />
• Dedicated custom antibody personnel actively manage the supply chain for full transparency from<br />
initial enquiries to production and post-sales collaborative support. Key features of the supply chain<br />
process include:<br />
– Specific lots of antibody material can be reserved for long-term multiple purchases<br />
– Custom packaging ensures that the material is available in an experiment-or<br />
platform-ready format<br />
– <strong>CST</strong> freezer-to-customer traceability ensures peace of mind and guarantees product integrity<br />
and performance<br />
– Priority Alert Plus shipping with SenseAware SM technology from FedEx minimizes product transit<br />
time and allows continuous monitoring of temperature during shipment<br />
– Scientists specializing in unique application support are available to support custom<br />
product clients<br />
• All catalog antibodies are available in bulk volumes, which ensures long term supply.<br />
Lot reservation is available to reserve a single lot of any of our reagents.<br />
Phospho-Stat1 (Tyr701) (58D6) Rabbit mAb #9167: Peptide ELISA analysis of multiple lots of #9167, using plates<br />
coated with phospho-peptides (Tyr-P) or non-phospho-peptides (NP). The data shows minimal lot-to-lot variation.<br />
278 For Research Use Only. Not For Use in Diagnostic Procedures. See pages 302 & 303 for Pathway Diagrams, Application, and Reactivity keys.<br />
<strong>CST</strong> Custom Formulated Antibodies<br />
<strong>CST</strong>’s primary antibodies are available in a carrier free formulation at custom concentrations that allow antibody<br />
labeling, conjugation or custom assays. Visit our website to learn more. www.cellsignal.com/custom<br />
www.cellsignal.com/customization<br />
279
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