CST Guide:
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II<br />
Antibody<br />
Applications<br />
Studies of cellular regulation may employ a variety of immunodetection<br />
methods, with sample types spanning cultured cells, primary cells,<br />
and tissue samples and their lysates. Using multiple applications for<br />
investigation provides valuable complementary information and confirms<br />
data validity. The insights gained can, in turn, help you communicate a<br />
more comprehensive understanding of cellular signaling mechanisms to<br />
the research community. In this context it is important to understand the<br />
strengths and limitations of each method. At Cell Signaling Technology,<br />
it is our philosophy to share all our methods and tips that help ensure<br />
successful experiments. In this section of the <strong>CST</strong> <strong>Guide</strong>, we discuss<br />
and illustrate best practice approaches to overcoming obstacles commonly<br />
faced when performing antibody-based applications.<br />
Section Includes:<br />
Tips for Success<br />
Application Protocols<br />
Troubleshooting <strong>Guide</strong>s<br />
Protein Synthesis<br />
Key components of protein synthesis, including transcription and translational machinery,<br />
are illustrated at the junction between the nuclear pore and endoplasmic reticulum.<br />
www.cellsignal.com/cstlandscapes<br />
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