Lung function measurements in children - copsac
Lung function measurements in children - copsac
Lung function measurements in children - copsac
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identity <strong>in</strong> a postal questionnaire (13, 14) and confirmed by DNA analysis with 10 highly<br />
polymorphic marker <strong>in</strong> our study population.<br />
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The Danish National Patient Registry (DNPR) records all hospitalizations based on the 10th<br />
revision of the "International Statistical Classification of Diseases and Related Health<br />
Problems" (ICD10).<br />
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A research database was established between January 1996 and May 2003 record<strong>in</strong>g RSV test<br />
from all hospitalizations <strong>in</strong> Denmark (15).<br />
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Study population<br />
The target population was identified by l<strong>in</strong>k<strong>in</strong>g the personal identification number to 1) the<br />
tw<strong>in</strong> status, 2) liv<strong>in</strong>g address and 3) hospitalizations due to the diagnoses RSV pneumonia<br />
(J12.1), RSV bronchitis (J20.5), RSV bronchiolitis (J21.0), and other diseases caused by RSV<br />
(B97.4) dur<strong>in</strong>g the period 1/1-1994 to 31/12-2003. The registry assures that the control tw<strong>in</strong><br />
had never been hospitalized with RSV <strong>in</strong>fection. Hospital records were retrieved to verify<br />
respiratory symptoms compatible with RSV bronchiolitis (severe cough, positive X-ray of<br />
thorax, use of 2-agonist, crackles or wheeze by auscultation of lungs) (16, 17) and verified<br />
with an enzyme-l<strong>in</strong>ked immunosorbent assay or immunofluorescence assay test for RSV.<br />
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Cl<strong>in</strong>ical exam<strong>in</strong>ation<br />
Tw<strong>in</strong>s and their parents were summoned to the Danish Pediatric Asthma Center two centers <strong>in</strong><br />
East- and West- Denmark for cl<strong>in</strong>ical exam<strong>in</strong>ation <strong>in</strong>clud<strong>in</strong>g <strong>in</strong>terviews on the child’s asthma<br />
accord<strong>in</strong>g to the GINA guidel<strong>in</strong>es (18); <strong>in</strong>formation about medical history and objective<br />
assessments. The <strong>in</strong>terviews and cl<strong>in</strong>ical exam<strong>in</strong>ations were done by one physician (PP).<br />
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