Lung function measurements in children - copsac
Lung function measurements in children - copsac
Lung function measurements in children - copsac
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Methods<br />
Designs<br />
Study II:<br />
Hospital records were retrieved to verify respiratory symptoms compatible with RSV<br />
bronchiolitis (severe cough, positive X-ray of thorax, use of 2-agonist, crackles or<br />
wheeze by auscultation of lungs) (85, 127) and verified with an enzyme-l<strong>in</strong>ked<br />
immunosorbent assay (ELISA) or immunofluorescence assay test for RSV.<br />
The tw<strong>in</strong> pairs and their parents were summoned to the Danish Pediatric Asthma Center<br />
two centers <strong>in</strong> East- and West- Denmark for cl<strong>in</strong>ical exam<strong>in</strong>ation <strong>in</strong>clud<strong>in</strong>g <strong>in</strong>terviews<br />
on the child’s asthma accord<strong>in</strong>g to the GINA guidel<strong>in</strong>es (128); <strong>in</strong>formation about<br />
medical history and objective assessments. The <strong>in</strong>terviews and cl<strong>in</strong>ical exam<strong>in</strong>ations<br />
were done by one physician (PP). Twenty-two pairs were exam<strong>in</strong>ed <strong>in</strong> Copenhagen and<br />
15 tw<strong>in</strong> pairs <strong>in</strong> Aarhus.<br />
Sk<strong>in</strong> prick test was done with standard <strong>in</strong>halation allergens: birch, mugwort, grass,<br />
horse, dog, cat, dust mite and mould (Soluprick, SQ ALK-Abelló A/S) (129).<br />
Study III:<br />
The COPSAC cohort was assessed at one center (the Danish Pediatric Asthma Center,<br />
Copenhagen).<br />
Bronchiolitis<br />
Bronchiolitis was def<strong>in</strong>ed as an acute respiratory illness before the age of 2 years based<br />
on symptoms of coryza progress<strong>in</strong>g over a few days to cough, tachypnea, chest<br />
retraction and wide spread crackles, wheezes or both (127) diagnosed by the COPSAC<br />
cl<strong>in</strong>ical research unit doctor or admitted acutely to the local hospital for such symptoms.<br />
RSV bronchiolitis<br />
RSV bronchiolitis was def<strong>in</strong>ed as bronchiolitis with a positive ELISA test for RSV<br />
<strong>in</strong>fection with<strong>in</strong> 10 days of the acute episode.<br />
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