Cell Line Development & Engineering

Monday, May 20, 2013 (continued)
3:00 CASE STUDY • UNPUBLISHED DATA Proteomic Analysis of Chinese
Hamster Ovary (CHO) Cells
To complement the recent genomic sequencing of Chinese hamster ovary
(CHO) cells, proteomic analysis was performed on CHO cells including the
cellular proteome, secretome, and glycoproteome using mass spectrometry
and multiple strategies. A total of 6164 grouped proteins were identified. This
first large-scale proteomic analysis enhances the knowledge base about CHO
capabilities for recombinant expression and cell line engineering.
Deniz Baycin Hizal, Ph.D., Chemical and Biomolecular Engineering,
Johns Hopkins University
3:30 Networking Refreshment Break and Poster/Exhibit Viewing
Proliferation and Integration of Data Sets
4:00 Integrated miRNA, mRNA and Protein Expression Analysis
Reveals the Role of Post-Transcriptional Regulation in
Controlling CHO Cell Growth Rate
To investigate the role of microRNA (miRNA) in the regulation of Chinese
hamster ovary (CHO) cell growth, qPCR, microarray and quantitative LC-MS/
MS analysis were utilised for simultaneous expression profiling of miRNA, mRNA
and protein. In this presentation, the biological processes found to be correlated
with CHO cell growth rate is discussed and the analysis of multiple datasets to
identify potential miRNA-mediated regulation demonstrated.
Colin Clarke, Ph.D., Postdoc, National Institute for Cellular Biotechnology,
Dublin City University, Ireland
4:30 UNPUBLISHED DATA Using Metabolite Profiling Data to Make a
Difference to CHO Cell Bioprocesses
Metabolite profiling offers an ‘omics approach that enables a very
immediate read-out of the status of CHO cells in culture. From an
integrated analysis of profile changes, interpretations can be attained of
metabolic phenotype associated with growth and/or recombinant protein
production. This presentation illustrates our molecular understanding of
relationships between feeding, cellular metabolism and desirable phenotype
in CHO cells.
Alan Dickson, Ph.D., Director, Centre of Excellence in Biopharmaceuticals,
Professor of Biotechnology, University of Manchester, United Kingdom
5:00 UNPUBLISHED DATA Genome-Scale Analysis of Chinese Hamster
Ovarian Cell Lines
Over the past 15 years, microbe-based engineering has advanced through
three major innovations: 1) genome sequencing, 2) genome-scale metabolic
models, and 3) tools for genome editing. These have allowed engineers to
identify cellular parts, simulate product synthesis, and manipulate host
genomes to enhance production. Similar advances are now upon us in the
engineering of CHO cell lines for bioprocessing.
Bernhard Palsson, Ph.D., Principal Investigator, Galletti Professor of
Bioengineering, Adjunct Professor Medicine, University of California, San Diego
5:30 Cocktail Reception in Poster/Exhibit Hall
Tuesday, May 21, 2013
7:30 Coffee
8:00 Chairman’s Opening Remarks
Rodney Combs, M.S., Associate Research Fellow, Bioprocess R&D, Culture
Process Development, World Wide Pharmaceutical Sciences, Pfizer Inc.
Approaches to Improve Process and Product Quality
8:15 CASE STUDY Clonality – Challenges, Approaches and
Lessons Learned
Abstract not available at time of print. Please visit
www.IBCLifeSciences.com/CellLine for updates.
Pamela Hawley-Nelson, Ph.D., Associate Director, Process Cell Culture,
MedImmune
8:45 Use of QPCR and DNA Sequencing Tools to Ensure
Product Quality and Safety
Quantitative PCR (Q-PCR) and DNA sequencing are tools that enable rapid,
sensitive and precise quantitation, detection and identification of critical
cellular and process impurities in cell culture manufacturing and product
purification. Additionally, these tools can be utilized in development and
characterization of production cell lines and in routine monitoring of cell line
stability. In this presentation, the applications of these technologies and present
data demonstrating the performance of assays for impurity assessment, cell line
characterization, contaminant detection and identification are reviewed.
Michael T. Brewer, Director, Head of Pharma Analytics, Life Technologies
9:15 UNPUBLISHED DATA High-Throughput Product Quality Assays for
Cell Line and Process Development
Here we present high-throughput (HTP) analytical assays to facilitate rapid
product quality using 96-well plate formats. These HTP product quality assays
include HTP protein quantitation followed by HTP protein purification and
product quality analyses. With these HTP analytical product quality assays we can
assess product quality in the early stage of clone screening, as well as expedite the
cell line and process development.
Shashi Prajapati, Ph.D., Senior Scientist, Cell Culture Development,
High Throughput Analytical Group. Biogen Idec
9:45 Networking Refreshment Break and Poster/Exhibit Viewing
10:15 CASE STUDY • UNPUBLISHED DATA Impact of Media Components and
Process Parameters on Product Color
Health Authorities expect that companies monitor and control the color
of liquid formulations of monoclonal antibodies. Cell culture conditions
and media components were investigated and shown to influence color.
Mechanisms that could explain these effects are discussed. Some process
changes that reduced color also decreased titer, and strategies for reducing
color which avoid productivity impact are discussed.
Natarajan Vijayasankaran, Ph.D., Senior Engineer, Late Stage Cell Culture,
Genentech, Inc.
10:45 CASE STUDY • UNPUBLISHED DATA Viability and Productivity
Improvement on Mammalian Fed Batch Culture
Abstract not available at time of print. Please visit
www.IBCLifeSciences.com/CellLine for updates.
Wenge Wang, Ph.D., Senior Principal Scientist, Bioprocess Research and
Development, Pfizer Inc.
11:15 Technology Workshop
A New Bench Scale Single-Use
Bioreactor System
Development of a new bench scale “rocker style” single use bioreactor
will be described, with discussion of physical parameters for design space
definition and performance measurement; with comparison to industry
standard systems. Presentation will conclude with results from CHO
batch culture trials for design validation, showing significant increases in
achievable cell densities and cell productivity.
Charles G. Golightly, Global Product Manager, Pall Life Sciences
11:45 Luncheon and Poster/Exhibit Viewing
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