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World Congress of Malacology Antwerp ... - Unitas Malacologica

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were able to detect weak genetic differentiation among populations. Three distinct areas were<br />

distinguished based on the presence <strong>of</strong> private alleles and allele frequencies: Cantabric, Miño basin<br />

and Duero-Tagus drainages. Within the latter group, results for the Alberche river population (Tagus<br />

basin) support the hypothesis that it was recently ‘captured’ from the Duero drainage basin by the<br />

Tagus basin. These genetic data, together with ecological and phylogeographical data, provide<br />

information critical to the development <strong>of</strong> appropriate conservation programs for this species.<br />

Margaritifera marocana Pallary, 1918, a distinct species <strong>of</strong> Margaritifera still survives in<br />

Morocco<br />

Toledo, Carlos 1 ; Van Damme, Dirk 2 ; Araujo, Rafael 1 ; Machordom, Annie 1<br />

1. Museo Nacional de Ciencias Naturales (CSIC). José Gutiérrez Abascal 2. 28006 Madrid, Spain,<br />

Email: rafael@mncn.csic.es; carlostc@mncn.csic.es; annie@mncn.csic.es<br />

2. Research Unit Paleontology, Gent University. Krijgslaan 281, B-9000 Gent. Belgium,<br />

Email: Dirk.VanDamme@UGent.be<br />

Among the Unionoida, the family Margaritiferidae is a relict group for which the number <strong>of</strong> genera<br />

and species is under discussion. Two species <strong>of</strong> the genus Margaritifera live in the West Palearctic:<br />

Margaritifera margaritifera and M. auricularia, both <strong>of</strong> which are endangered across their respective<br />

distributions. M. auricularia only lives in the Ebro basin in Spain, the Loire and Garonne basins in<br />

France and in Morocco. Populations from the latter area are regarded as a distinct subspecies (M.<br />

auricularia marocana) based on conchology.<br />

We have had the opportunity to analyse samples from Morocco, and compare their sequences with<br />

the other studied species <strong>of</strong> the same family. Partial sequences <strong>of</strong> the mitochondrial genes<br />

cytochrome oxidase subunit I (CoI) and 16S rRNA (16S) support the recognition <strong>of</strong> the Moroccan<br />

population as a different lineage. The divergence found between the Iberian and Moroccan<br />

populations <strong>of</strong> M. auricularia (9.4% for CoI and 6.3% for 16S) is <strong>of</strong> the same order as that between<br />

M. falcata and M. laevis (7.9% for CoI and 4.1% for 16S) or between M. margaritifera and M. laevis<br />

(9.9% for CoI and 4.7% for 16S).<br />

Though further morphological and ecological studies must be undertaken, our molecular data clearly<br />

support the recognition <strong>of</strong> the specimens from Morocco as representing a distinct species. Until<br />

further work disentangles the taxonomic organization <strong>of</strong> Moroccan populations we propose the name<br />

Margaritifera marocana Pallary, 1918.<br />

In addition, a similar comparative study is also required to establish the degree <strong>of</strong> relatedness<br />

between the two surviving populations <strong>of</strong> M. auricularia from Spain and France in order to complete<br />

our knowledge about these relict populations <strong>of</strong> a very endangered species.<br />

Phylogenetic relationships <strong>of</strong> the southeast Asian land operculate snails <strong>of</strong> the genus<br />

Cyclophorus (Prosobranchia: Cyclophoridae) using DNA sequence data<br />

Tongkerd, Piyoros 1 , Sutcharit, Chirasak 1 , Douzery, Emmanuel 2 , Jaeger, Jean-Jacques 2 ; Panha,<br />

Somsak 1<br />

1. Animal Systematics Research Unit, Department <strong>of</strong> Biology, Faculty <strong>of</strong> Science, Chulalongkorn<br />

University, Bangkok 10330, Thailand,<br />

Email: somsakp@sc.chula.ac.th, piyoros_tongkerd@yahoo.com, jirasak4@yahoo.com<br />

2. Phylogénie Paléobiologie Paléontologie, Institut des Sciences de l’Evolution, Université Sciences<br />

et Techniques du Languedoc (Montpellier II), CC 064, Université Montpellier II, Place Eugene<br />

Bataillon 34905 Montpellier Cedex 05, France,<br />

Email: douzery@isem.univ-montp2.fr, Jaeger@isem.univ-montp2.fr<br />

A molecular phylogeny is presented for part <strong>of</strong> the Southeast Asian land operculate snails <strong>of</strong> the<br />

genus Cyclophorus Montfort, 1810 (Prosobranchia: Cyclophoridae) based on DNA sequences data<br />

from the mitochondrial cytochrome oxidase 1 locus. It has at least 10 parapatrically distributed colour<br />

223

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