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World Congress of Malacology Antwerp ... - Unitas Malacologica

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asal origin <strong>of</strong> species from Sulawesi, and a derivation <strong>of</strong> Australian taxa from the Southeast Asian<br />

mainland. Low genetic differences among the species or populations render the assumption <strong>of</strong> an<br />

intralacustrine radiation in ancient lake Inlé in Myanmar unlikely, while for Lake Poso on Sulawesi<br />

the data at least suggest the occurrence <strong>of</strong> cryptic lacustrine species.<br />

Crystallographic textures <strong>of</strong> shell layers in Patellogastropoda: a new tool unraveling their<br />

phylogeny<br />

Frýda, Jiří 1 ; Sasaki, Takenori 2<br />

1. Czech Geological Survey, Klárov 3/131, 118 21 Praha 1, Czech Republic,<br />

Email: bellerophon@seznam.cz<br />

2. The University Museum, The University <strong>of</strong> Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo 113-0033,<br />

Email: sasaki@um.u-tokyo.ac.jp<br />

Limpet shells belonging to the Patellogastropoda have the most diversified microstructural pattern<br />

among all living gastropods and data on their mineralogy and microstructure have been frequently<br />

used for their classification. In contract to microstructure (characterizing a shape <strong>of</strong> biocrystals) there<br />

are almost no data on crystallographic textures <strong>of</strong> patellogastropod shells (characterizing an<br />

arrangement <strong>of</strong> crystal axes <strong>of</strong> individual biocrystals). Shell layers having the same microstructure<br />

can have different crystallographic textures and vice versa. Thus, analyses <strong>of</strong> crystallographic texture<br />

and microstructure <strong>of</strong> patellogastropod shells provide non-redundant characters. To fill a gap in our<br />

knowledge on the crystallographic textures <strong>of</strong> patellogastropod shells, we have applied several<br />

diffraction techniques providing complementary information about the crystallographic texture and<br />

mineralogy <strong>of</strong> their shells. We analyzed individual shell layers <strong>of</strong> 14 patellogastropod species<br />

belonging to Lottiidae (Niveotectura, Patelloida, Lottia, and Nipponacmea), Patellidae (Scutellastra),<br />

Nacellidae (Cellana), Lepetidae (Limalepeta), and Acmaeidae (Pectinodonta). Our analysis has<br />

revealed a large variety <strong>of</strong> textural patterns <strong>of</strong> the patellogastropod shells. The crystallographic<br />

texture pattern in corresponding shell layers <strong>of</strong> closely related taxa is identical but different in<br />

distantly related taxa. This fact confirms that the crystallographic texture is under biological control<br />

as shell microstructure and it is not driven only by abiological forces (like a mineral epitaxy). The<br />

crystallographic texture pattern represents characters useful for a phylogenetic reconstruction <strong>of</strong> the<br />

higher levels <strong>of</strong> the classification <strong>of</strong> the Patellogastropoda. In addition, our analysis suggests that<br />

textural analysis together with mineralogical and microstructural data can help resolve problems <strong>of</strong><br />

homology <strong>of</strong> individual shell layers in the patellogastropod shells.<br />

“Niku-nuki”, a useful method for preparation <strong>of</strong> shell-bearing molluscan specimens<br />

Fukuda, Hiroshi 1 ; Haga, Takuma 2 ; Tatara, Yuki 3<br />

1. Conservation <strong>of</strong> Aquatic Biodiversity, Faculty <strong>of</strong> Agriculture, Okayama University, Tsushimanaka<br />

1-1-1, Okayama 700-8530, Japan,<br />

Email: suikei1@cc.okayama-u.ac.jp<br />

2. Department <strong>of</strong> Biological Science, Graduate School <strong>of</strong> Sciences, The University <strong>of</strong> Tokyo, 7-3-1<br />

Hongo, Bunkyo-ku, Tokyo 113-0033, Japan,<br />

Email: haga@kahaku.go.jp<br />

3. Department <strong>of</strong> Biology, Faculty <strong>of</strong> Science, Toho University, Miyama 2-2-1, Funabashi 274-8510,<br />

Japan,<br />

Email: ykui@msc.biglobe.ne.jp<br />

Often only one or a few individuals <strong>of</strong> rare species are collected. How do we treat them as intact<br />

voucher specimens? The shell <strong>of</strong> the whole individual in formalin or alcohol will corrode or fade. In<br />

order to dissect the s<strong>of</strong>t parts, you must crack or dissolve the shell.<br />

“Niku-nuki”, a traditional method used by Japanese malacologists overcomes this dilemma. It is<br />

applicable to minute molluscs. The outline is:<br />

65

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