World Congress of Malacology Antwerp ... - Unitas Malacologica
World Congress of Malacology Antwerp ... - Unitas Malacologica
World Congress of Malacology Antwerp ... - Unitas Malacologica
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asal origin <strong>of</strong> species from Sulawesi, and a derivation <strong>of</strong> Australian taxa from the Southeast Asian<br />
mainland. Low genetic differences among the species or populations render the assumption <strong>of</strong> an<br />
intralacustrine radiation in ancient lake Inlé in Myanmar unlikely, while for Lake Poso on Sulawesi<br />
the data at least suggest the occurrence <strong>of</strong> cryptic lacustrine species.<br />
Crystallographic textures <strong>of</strong> shell layers in Patellogastropoda: a new tool unraveling their<br />
phylogeny<br />
Frýda, Jiří 1 ; Sasaki, Takenori 2<br />
1. Czech Geological Survey, Klárov 3/131, 118 21 Praha 1, Czech Republic,<br />
Email: bellerophon@seznam.cz<br />
2. The University Museum, The University <strong>of</strong> Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo 113-0033,<br />
Email: sasaki@um.u-tokyo.ac.jp<br />
Limpet shells belonging to the Patellogastropoda have the most diversified microstructural pattern<br />
among all living gastropods and data on their mineralogy and microstructure have been frequently<br />
used for their classification. In contract to microstructure (characterizing a shape <strong>of</strong> biocrystals) there<br />
are almost no data on crystallographic textures <strong>of</strong> patellogastropod shells (characterizing an<br />
arrangement <strong>of</strong> crystal axes <strong>of</strong> individual biocrystals). Shell layers having the same microstructure<br />
can have different crystallographic textures and vice versa. Thus, analyses <strong>of</strong> crystallographic texture<br />
and microstructure <strong>of</strong> patellogastropod shells provide non-redundant characters. To fill a gap in our<br />
knowledge on the crystallographic textures <strong>of</strong> patellogastropod shells, we have applied several<br />
diffraction techniques providing complementary information about the crystallographic texture and<br />
mineralogy <strong>of</strong> their shells. We analyzed individual shell layers <strong>of</strong> 14 patellogastropod species<br />
belonging to Lottiidae (Niveotectura, Patelloida, Lottia, and Nipponacmea), Patellidae (Scutellastra),<br />
Nacellidae (Cellana), Lepetidae (Limalepeta), and Acmaeidae (Pectinodonta). Our analysis has<br />
revealed a large variety <strong>of</strong> textural patterns <strong>of</strong> the patellogastropod shells. The crystallographic<br />
texture pattern in corresponding shell layers <strong>of</strong> closely related taxa is identical but different in<br />
distantly related taxa. This fact confirms that the crystallographic texture is under biological control<br />
as shell microstructure and it is not driven only by abiological forces (like a mineral epitaxy). The<br />
crystallographic texture pattern represents characters useful for a phylogenetic reconstruction <strong>of</strong> the<br />
higher levels <strong>of</strong> the classification <strong>of</strong> the Patellogastropoda. In addition, our analysis suggests that<br />
textural analysis together with mineralogical and microstructural data can help resolve problems <strong>of</strong><br />
homology <strong>of</strong> individual shell layers in the patellogastropod shells.<br />
“Niku-nuki”, a useful method for preparation <strong>of</strong> shell-bearing molluscan specimens<br />
Fukuda, Hiroshi 1 ; Haga, Takuma 2 ; Tatara, Yuki 3<br />
1. Conservation <strong>of</strong> Aquatic Biodiversity, Faculty <strong>of</strong> Agriculture, Okayama University, Tsushimanaka<br />
1-1-1, Okayama 700-8530, Japan,<br />
Email: suikei1@cc.okayama-u.ac.jp<br />
2. Department <strong>of</strong> Biological Science, Graduate School <strong>of</strong> Sciences, The University <strong>of</strong> Tokyo, 7-3-1<br />
Hongo, Bunkyo-ku, Tokyo 113-0033, Japan,<br />
Email: haga@kahaku.go.jp<br />
3. Department <strong>of</strong> Biology, Faculty <strong>of</strong> Science, Toho University, Miyama 2-2-1, Funabashi 274-8510,<br />
Japan,<br />
Email: ykui@msc.biglobe.ne.jp<br />
Often only one or a few individuals <strong>of</strong> rare species are collected. How do we treat them as intact<br />
voucher specimens? The shell <strong>of</strong> the whole individual in formalin or alcohol will corrode or fade. In<br />
order to dissect the s<strong>of</strong>t parts, you must crack or dissolve the shell.<br />
“Niku-nuki”, a traditional method used by Japanese malacologists overcomes this dilemma. It is<br />
applicable to minute molluscs. The outline is:<br />
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