3. Nucleic Acids Electrophoresis - Euroclone
3. Nucleic Acids Electrophoresis - Euroclone
3. Nucleic Acids Electrophoresis - Euroclone
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3 <strong>Nucleic</strong> <strong>Acids</strong> <strong>Electrophoresis</strong><br />
Acrylamides<br />
For the separation of oligonucleotides with a small number of bases,<br />
acrylamide gels with a small pore size are used. The pore size is<br />
determined by the total concentration of acrylamide and the ratio of<br />
Acrylamide/Bisacrylamide. For the sequencing of nucleic acids a ratio of<br />
Acrylamide/Bisacrylamide 19:1 is used. EuroClone offers a ready-to-use<br />
40% Acrylamide/Bisacrylamide 19:1 mix.<br />
Cat. Description Format<br />
EMR013500 Acrylamide/Bisacrylamide 19:1 (solution 40%) 500 ml<br />
EMR013001 Acrylamide/Bisacrylamide 19:1 (solution 40%) 1 L<br />
Buffers and reagents for DNA electrophoresis<br />
EuroSafe – <strong>Nucleic</strong> Acid Stain<br />
EuroSafe <strong>Nucleic</strong> Acid Staining Solution (20,000x) is a safe nucleic acid<br />
stain, an alternative to the traditional ethidium bromide (EtBr) stain<br />
for detecting nucleic acid in agarose gels. It emits green fluorescence<br />
when bound to DNA or RNA. This stain has two fluorescence excitation<br />
maxima when bound to nucleic acid, one centered at 309 nm and<br />
another at 419 nm. In addition, it has one visible excitation at 514<br />
nm. The fluorescence emission of EuroSafe bound to DNA is centered<br />
at 537 nm. EuroSafe is as sensitive as EtBr. Compared to EtBr, known<br />
as a strong mutagen it causes much fewer mutations in the Ames<br />
test. In addition, EuroSafe <strong>Nucleic</strong> Acid Staining Solution (20,000x)<br />
has a negative result in mouse marrow chromophilous erythrocyte<br />
micronucleus test and mouse spermary spermatocyte chromosomal<br />
aberration test.<br />
Features<br />
Used for detecting DNA and RNA<br />
Alternative to the ethidium bromide staining<br />
As sensitive as EtBr or more sensitive than that<br />
Less mutagenic than EtBr<br />
Cat. Description Format<br />
EMR440001 EuroSafe 1 ml<br />
Storage and Stability<br />
Two years at Room Temperature<br />
Shipping<br />
Room Temperature<br />
For the complete list of EuroClone Biochemicals<br />
please refer to Chapter 7 or visit<br />
www.euroclone.it<br />
Cat. Description Format Cat. Description Format<br />
EMR100001 Boric acid 1 kg EMR224001 TBE Buffer, 10X - MBG 1 L<br />
EMR100005 Boric acid 5 kg EMR224500 TBE Buffer, 10X - MBG 500 ml<br />
EMR130001 EDTA disodium salt dihydrate 1 kg EMR042001 TBE Buffer, 10X - powder 1 L<br />
EMR130500 EDTA disodium salt dihydrate 500 g EMR227001 TE Buffer pH 8.0, 1X 1 L<br />
EMR034001 EDTA - Solution pH 8.0 (0,5 M) 1 L EMR226001 TE Buffer pH8.0, 100X 1 L<br />
EMR034500 EDTA - Solution pH 8.0 (0,5 M) 500 ml EMR226500 TE Buffer pH8.0, 100X 500 ml<br />
EMR036015 Ethidium Bromide 0,07% 15 ml EMR045001 Tris 1 kg<br />
EMR035025 Ethidium Bromide 1% 25 ml EMR045500 Tris 500 g<br />
EMR063001 TAE Buffer 10X 1 L EMR270001 Tris-HCl 1 kg<br />
EMR064001 TAE Buffer 50X 1 L EMR270500 Tris-HCl 500 g<br />
EMR427005 TBE Buffer, 10X 5 L<br />
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