3. Nucleic Acids Electrophoresis - Euroclone

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3 Nucleic Acids Electrophoresis Agaroses EuroClone offers a complete selection of high quality agaroses for standard and specific electrophoresis appications. GellyPhor ® LE GellyPhor ® LM GellyPhor ® ULTRA GellyPhor ® HR GellyPhor ® PFGE DNA separation 1-50 kb - - - - ✓ DNA/RNA separation ≤ 1 kb - - ✓ ✓ - DNA/RNA separation ≥1 kb ✓ ✓ - . ✓ DNA separation 20-800 bp - - ✓ - - Blotting ✓ - - ✓ ✓ DNA finger printing ✓ - - - - Nucleic Acid Recovery ✓ ✓ ✓ ✓ ✓ GellyPhor ® LE GellyPhor ® LE is ideal for general nucleic acids preparative and analytical gel electrophoresis as well as for blotting. This gel sharply resolves the different DNA fragments to be analyzed and provides consistent resolution from batch-to-batch. It forms high strength gels with low background upon ethidium bromide staining. Due to the low electroendosmosis value (EEO) of GellyPhor ® LE the DNA has a high electrophoretic mobility allowing shorter running time. Technical Specification Gelling Temperature (dynamic measurement in 1.5% solution) 34-38 °C Gel strength (1%) ≥1200 g/cm 2 Electroendosmosis (-m r ) ≤ 0.12 Sulphate ≤ 0.14% Moisture ≤ 8.5% Residue on ignition ≤ 0.60% RNase/DNase Activity none detected Cat. Description Format EMR010100 GellyPhor ® LE 100 g EMR010500 GellyPhor ® LE 500 g EMR010001 GellyPhor ® LE 1 kg Storage and Stability Five years at Room Temperature Shipping Room Temperature GellyPhor ® LM GellyPhor ® LM is a low melting temperature agarose. This molecular biology grade agarose produces gels with greater sieving properties and higher clarity than standard melting temperature agarose. The low melting temperature of GellyPhor ® LM makes it ideal for preparative nucleic acids electrophoresis, while its low geling temperature is perfect for cloning of tissue culture cells and viral plaque assays. Cat. Description Format EMR911100 GellyPhor ® LM 100 g Storage and Stability Five years at Room Temperature Shipping Room Temperature Technical Specification Gelling Temperature (dynamic measurement in 1.5% solution) 24-28 °C Gel strength (1,5%) ≥ 500 g/cm 2 Electroendosmosis (-m r ) ≤ 0.12 Sulphate ≤ 0.12% Moisture ≤ 7% Melting Temperature (1.5%) ≤ 65.5 °C RNase/DNase Activity none detected 16
Nucleic Acids Electrophoresis 3 GellyPhor ® HR GellyPhor ® HR is a molecular biology grade standard melting temperature agarose, that yields strong gels for fine resolution of small nucleic acids fragments. Performance testing of GellyPhor ® HR ensures fine resolution of DNA fragments up to 1000 bp, though this agarose is capable of finely resolving DNA fragments ranging from 10 bp up to 1200 bp. GellyPhor ® HR is designed for analytical electrophoresis. Technical Specification Gelling Temperature (dynamic measurement in 3% solution) Gel strength 35.5 °C Electroendosmosis (-mr) ≤ 0.12 Sulphate ≤ 0.11% Moisture ≤ 7% ≥ 600 g/cm 2 (for a 1.5% gel) ≥ 1500 g/cm 2 (for a 3% gel) Melting Temperature (3%) ≤ 80 °C RNase/DNase Activity none detected Ranges of separation: 1.8% 400-1200 bp 3% 150-800 bp 4.5% 15-400 bp These ranges are approximate and have been calculated in TAE Buffer. To achieve the best resolution of GellyPhor ® HR gels, they should be stored at 4 °C- 8 °C for 30 minutes before use. Cat. Description Format EMR912100 GellyPhor ® HR 100 g Storage and Stability Five years at Room Temperature Shipping Room Temperature GellyPhor ® ULTRA GellyPhor ® ULTRA is an intermediate melting temperature agarose that provides twice the resolution capabilities of the finest sieving agarose products. You can resolve DNA fragments, PCR and RT-PCR products differing in size by 2%, in the range of 200 bp to 800 bp, by horizontal gel electrophoresis (for example, a 200 bp DNA fragment can be separated from a 204 bp fragment). Using fast run protocols, DNA fragments differing in size by 1% can be resolved in as little as 1.5 hours in a 20 cm long horizontal or vertical gel format. GellyPhor ® ULTRA agarose gels (2% to 4%) approximate the resolution of polyacrylamide gels (4% to 8%). Cat. Description Format EMR915100 GellyPhor ® ULTRA 125 g Storage and Stability Five years at Room Temperature Shipping Room Temperature Technical Specification Gelling Temperature (dynamic measurement in 3% solution) ≤ 36 °C Gel strength (3%) ≥ 400 g/cm 2 Electroendosmosis (-m r ) 0.02 – 0.05 Moisture ≤ 10% Melting Temperature (3%) ≤ 75 °C RNase/DNase Activity none detected GellyPhor ® PFGE GellyPhor ® PFGE is a very high gel strength, low EEO, standard gelling temperature agarose. Due to its high gel strength, this agarose can be used for the preparation of low percentage gels for the analysis not only of high molecular weight nucleic acids (such as chromosomes), but also of largesized particles such as viruses and ribosomes. Technical Specification Gelling Temperature (dynamic measurement in 1.5% solution) Gel strength 36 ± 1.5 °C Electroendosmosis (-m r ) 0.13 Moisture ≤ 7% ≥ 1600 g/cm 2 (for a 1% gel) ≥ 3000 g/cm 2 (for a 1.5% gel) Melting Temperature (1.5%) ≤ 1.5 °C RNase/DNase Activity none detected Cat. Description Format EMR916100 GellyPhor ® PFGE 100 g Storage and Stability Five years at Room Temperature Shipping Room Temperature 17
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3. Nucleic Acids Electrophoresis - Euroclone

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