3. Nucleic Acids Electrophoresis - Euroclone

3. Nucleic Acids Electrophoresis 

• Agaroses 

• Acrylamides 

• Buffers & Reagents for DNA Electrophoresis 

• DNA Ladders 

• Horizontal Electrophoresis System

3 Nucleic Acids Electrophoresis 

Agaroses 

EuroClone offers a complete selection of high quality agaroses for standard and specific electrophoresis appications. 

GellyPhor ® LE GellyPhor ® LM GellyPhor ® ULTRA GellyPhor ® HR GellyPhor ® PFGE 

DNA separation 1-50 kb - - - - ✓ 

DNA/RNA separation ≤ 1 kb - - ✓ ✓ - 

DNA/RNA separation ≥1 kb ✓ ✓ - . ✓ 

DNA separation 20-800 bp - - ✓ - - 

Blotting ✓ - - ✓ ✓ 

DNA finger printing ✓ - - - - 

Nucleic Acid Recovery ✓ ✓ ✓ ✓ ✓ 

GellyPhor ® LE 

GellyPhor ® LE is ideal for general nucleic acids preparative and analytical 

gel electrophoresis as well as for blotting. 

This gel sharply resolves the different DNA fragments to be analyzed 

and provides consistent resolution from batch-to-batch. It forms high 

strength gels with low background upon ethidium bromide staining. 

Due to the low electroendosmosis value (EEO) of GellyPhor ® LE the 

DNA has a high electrophoretic mobility allowing shorter running time. 

Technical Specification 

Gelling Temperature (dynamic measurement in 1.5% 

solution) 

34-38 °C 

Gel strength (1%) ≥1200 g/cm 2 

Electroendosmosis (-m r 

) ≤ 0.12 

Sulphate ≤ 0.14% 

Moisture ≤ 8.5% 

Residue on ignition ≤ 0.60% 

RNase/DNase Activity 

none 

detected 

Cat. Description Format 

EMR010100 GellyPhor ® LE 100 g 

EMR010500 GellyPhor ® LE 500 g 

EMR010001 GellyPhor ® LE 1 kg 

Storage and Stability 

Five years at Room Temperature 

Shipping 

Room Temperature 

GellyPhor ® LM 

GellyPhor ® LM is a low melting temperature agarose. This molecular 

biology grade agarose produces gels with greater sieving properties 

and higher clarity than standard melting temperature agarose. The low 

melting temperature of GellyPhor ® LM makes it ideal for preparative 

nucleic acids electrophoresis, while its low geling temperature is perfect 

for cloning of tissue culture cells and viral plaque assays. 


EMR911100 GellyPhor ® LM 100 g 



Shipping 



Gelling Temperature (dynamic measurement in 1.5% 

solution) 

24-28 °C 

Gel strength (1,5%) ≥ 500 g/cm 2 


) ≤ 0.12 


Moisture ≤ 7% 

Melting Temperature (1.5%) ≤ 65.5 °C 


none detected 

16

Nucleic Acids Electrophoresis 3 

GellyPhor ® HR 

GellyPhor ® HR is a molecular biology grade standard melting temperature 

agarose, that yields strong gels for fine resolution of small nucleic acids 

fragments. Performance testing of GellyPhor ® HR ensures fine resolution of 

DNA fragments up to 1000 bp, though this agarose is capable of finely 

resolving DNA fragments ranging from 10 bp up to 1200 bp. GellyPhor ® HR 

is designed for analytical electrophoresis. 


Gelling Temperature (dynamic measurement in 3% 

solution) 

Gel strength 

35.5 °C 

Electroendosmosis (-mr) ≤ 0.12 



≥ 600 g/cm 2 (for a 1.5% 

gel) 

≥ 1500 g/cm 2 (for a 3% 

gel) 

Melting Temperature (3%) ≤ 80 °C 


none detected 

Ranges of separation: 

1.8% 400-1200 bp 

3% 150-800 bp 

4.5% 15-400 bp 

These ranges are approximate and have been calculated in TAE Buffer. 

To achieve the best resolution of GellyPhor ® HR gels, they should be 

stored at 4 °C- 8 °C for 30 minutes before use. 


EMR912100 GellyPhor ® HR 100 g 



Shipping 


GellyPhor ® ULTRA 

GellyPhor ® ULTRA is an intermediate melting temperature agarose that 

provides twice the resolution capabilities of the finest sieving agarose 

products. You can resolve DNA fragments, PCR and RT-PCR products 

differing in size by 2%, in the range of 200 bp to 800 bp, by horizontal gel 

electrophoresis (for example, a 200 bp DNA fragment can be separated from 

a 204 bp fragment). Using fast run protocols, DNA fragments differing in size 

by 1% can be resolved in as little as 1.5 hours in a 20 cm long horizontal or 

vertical gel format. GellyPhor ® ULTRA agarose gels (2% to 4%) approximate 

the resolution of polyacrylamide gels (4% to 8%). 


EMR915100 GellyPhor ® ULTRA 125 g 



Shipping 



Gelling Temperature (dynamic measurement in 3% 

solution) 

≤ 36 °C 

Gel strength (3%) ≥ 400 g/cm 2 


) 0.02 – 0.05 


Melting Temperature (3%) ≤ 75 °C 


none detected 

GellyPhor ® PFGE 

GellyPhor ® PFGE is a very high gel strength, low EEO, standard gelling 

temperature agarose. Due to its high gel strength, this agarose can be used 

for the preparation of low percentage gels for the analysis not only of high 

molecular weight nucleic acids (such as chromosomes), but also of largesized 

particles such as viruses and ribosomes. 


Gelling Temperature (dynamic measurement in 

1.5% solution) 

Gel strength 

36 ± 1.5 °C 

Electroendosmosis (-m r ) 0.13 


≥ 1600 g/cm 2 (for a 1% gel) 

≥ 3000 g/cm 2 (for a 1.5% gel) 

Melting Temperature (1.5%) ≤ 1.5 °C 


none detected 


EMR916100 GellyPhor ® PFGE 100 g 



Shipping 


17


Acrylamides 

For the separation of oligonucleotides with a small number of bases, 

acrylamide gels with a small pore size are used. The pore size is 

determined by the total concentration of acrylamide and the ratio of 

Acrylamide/Bisacrylamide. For the sequencing of nucleic acids a ratio of 

Acrylamide/Bisacrylamide 19:1 is used. EuroClone offers a ready-to-use 

40% Acrylamide/Bisacrylamide 19:1 mix. 


EMR013500 Acrylamide/Bisacrylamide 19:1 (solution 40%) 500 ml 

EMR013001 Acrylamide/Bisacrylamide 19:1 (solution 40%) 1 L 

Buffers and reagents for DNA electrophoresis 

EuroSafe – Nucleic Acid Stain 

EuroSafe Nucleic Acid Staining Solution (20,000x) is a safe nucleic acid 

stain, an alternative to the traditional ethidium bromide (EtBr) stain 

for detecting nucleic acid in agarose gels. It emits green fluorescence 

when bound to DNA or RNA. This stain has two fluorescence excitation 

maxima when bound to nucleic acid, one centered at 309 nm and 

another at 419 nm. In addition, it has one visible excitation at 514 

nm. The fluorescence emission of EuroSafe bound to DNA is centered 

at 537 nm. EuroSafe is as sensitive as EtBr. Compared to EtBr, known 

as a strong mutagen it causes much fewer mutations in the Ames 

test. In addition, EuroSafe Nucleic Acid Staining Solution (20,000x) 

has a negative result in mouse marrow chromophilous erythrocyte 

micronucleus test and mouse spermary spermatocyte chromosomal 

aberration test. 

Features 

Used for detecting DNA and RNA 

Alternative to the ethidium bromide staining 

As sensitive as EtBr or more sensitive than that 

Less mutagenic than EtBr 


EMR440001 EuroSafe 1 ml 


Two years at Room Temperature 

Shipping 


For the complete list of EuroClone Biochemicals 

please refer to Chapter 7 or visit 

www.euroclone.it 

Cat. Description Format Cat. Description Format 

EMR100001 Boric acid 1 kg EMR224001 TBE Buffer, 10X - MBG 1 L 

EMR100005 Boric acid 5 kg EMR224500 TBE Buffer, 10X - MBG 500 ml 

EMR130001 EDTA disodium salt dihydrate 1 kg EMR042001 TBE Buffer, 10X - powder 1 L 

EMR130500 EDTA disodium salt dihydrate 500 g EMR227001 TE Buffer pH 8.0, 1X 1 L 

EMR034001 EDTA - Solution pH 8.0 (0,5 M) 1 L EMR226001 TE Buffer pH8.0, 100X 1 L 

EMR034500 EDTA - Solution pH 8.0 (0,5 M) 500 ml EMR226500 TE Buffer pH8.0, 100X 500 ml 

EMR036015 Ethidium Bromide 0,07% 15 ml EMR045001 Tris 1 kg 

EMR035025 Ethidium Bromide 1% 25 ml EMR045500 Tris 500 g 

EMR063001 TAE Buffer 10X 1 L EMR270001 Tris-HCl 1 kg 

EMR064001 TAE Buffer 50X 1 L EMR270500 Tris-HCl 500 g 

EMR427005 TBE Buffer, 10X 5 L 

18


DNA Ladders 

EuroClone has three different DNA Ladders for sizing and approximate 

quantification of double-stranded DNA fragments such us PCR products. 

The Ladders have extremely sharp bands and reference bands with higher 

DNA content for easy orientation. 

SHARPMASS 50 - DNA LADDER 

This 50 bp DNA-Ladder is composed of 13 chromatography-purified 

individual DNA ranging from 50 to 1000 bp. 

The 500 bp and 250 bp fragments have a higher DNA content and serve as 

size references. 


This Ladder includes many plasmids fully digested by restriction enzymes. 

This process leads to the creation of 10 bands suitable for use as molecular 

weight standards for agarose gel electrophoresis. The digested DNA includes 

fragments ranging from 100 to 1000 bp. The 500 bp fragment has enhanced 

brightness and can be used as reference point. 


EMR810050 SHARPMASS 50 50 μg 




EMR813100 SHARPMASS 100 PLUS 100 lanes 



SHARPMASS 100 PLUS - READY-TO- 

LOAD DNA LADDER 

This Ladder consists of 12 DNA fragments ranging from 100 bp to 

3 kb. It is designed to show virtually uniform spacing over a wide 

fragment range. The Ladder allows sizing and concentration estimate 

of DNA fragments on agarose gels generated by PCR or restriction 

digest. The fragment mix is supplied in ready-to-use format containing 

orange and blue tracking dyes. 


This Ladder includes many plasmids fully digested by restriction 

enzymes. This process leads to the creation of 13 bands suitable for 

use as molecular weight standards for agarose gel electrophoresis. The 

digested DNA includes fragments ranging from 250 to 10000 bp. For 

an easier reference, the fragment of 1000 bp generates a three times 

brighter band and the 3.000 bp generates a two times brighter band. 


One year at -20 °C 

Shipping 

Blue Ice 

19


Horizontal Electrophoresis 

Systems 

Minigel Systems 

EuroClone minigel systems are ideal for use with low to medium 

sample numbers. 

Designed for a quick and reproducible separations, these minigel 

systems are perfectly suited for the daily needs of the research 

laboratory. 

System Components 

• UV-Transmissible (UVT) Gel Tray with two Comb position 

• Two Combs 

Convenient Handling 

• Easy-to-use casting systems - gel directlly in the chamber by 90 degree 

rotation of the rubbers-seal-equipped gel tray 

• Labeled Combs for easy identification of Comb type 

• Venting slot prevents water condensation for clear gel viewing 

• Gel tray and chamber lid are made of UV-Transmissible acrylic glass 

enabling the gel to be viewed under UV excitation 

Accessories 

A wide range of Combs, UV-transmissible gel trays featuring 

various Comb positions, multi casting rails for pouring several gels 

simultaneously, adjustable universal casting rail to accomodate gel trays 

of various sizes are available to complement this range. 

Mini S 

Features 

Gel Size 7 x 8 cm 

Footprint 12 x 9.5 x 16 cm 

Sample Flexibility max. 24 

Buffer Volume 400 ml 

Gel Volume 30 ml 

Cat. 

EM40-0708 

The system Mini S includes: 

• Buffer Chamber with Platinum Electrodes 

• SuperSafe Lid with Attached Leads 

• Gasketed UV-Transmissible (UVT) Gel Tray 

• 2 Combs: 1.5 mm thickness (6,10 teeth) 

Descritpion 

Mini S Horizontal 

Accessories 

Cat. 

Descritpion 

EM40-0708-10C 10 tooth Comb, 1.0 mm thick, Sample volume: 18 µl 

EM40-0708-10D 10 tooth Comb, 1.2 mm thick, Sample volume: 26 µl 



EM40-0708-5C 5 tooth Combs, 1.0 mm thick, Sample volume: 42 µl 

EM40-0708-5D 5 tooth Combs, 1.5 mm thick, Sample volume: 64 µl 





EM40-0708-PD 2 tooth, preparative Comb, 1.5 mm thick 

EM40-0708-UVT 

RapidCast UVT Gel Tray 7 x 8 cm with two Comb slots & 

gasketed ends 

RapidCast Gel Caster 7 x 8 cm for use with 80-0708-RCGT 

EM40-0708-CST 

Allows for three gels to be cast simultaneously. CASTER ONLY 

EM40-0708-GK Gasket, 80-0708-RCGT, pk 2 

20


Mini M 

Features 






Cat. 

EM40-0911 

System Components: 




• 2 Combs: 1.5 mm thick (10, 14 teeth) 

Description 

Mini M Horizontal 

Accessories 

Cat. 

Description 











EM40-0911-18C 

18 tooth Comb, 2 x (8+1), 2 x microtiter format, 1.0 

mm thick, Sample volume: 11 µl 

EM40-0911-18D 

18 tooth Comb, 2 x (8+1), 2 x microtiter format, 1.5 

mm thick, Sample volume: 16 µl 

EM40-0911-9C 

9 tooth Comb, (8+1), 1 x microtiter format, 1.0 mm 

thick 

EM40-0911-9D 

9 tooth Comb, (8+1), 1 x microtiter format, 1.5 mm 

thick 

EM40-0911-PD 2 tooth, preparative Comb, 1.5 mm thick 

EM40-0911-UVT RapidCast UVT Gel Tray with two Comb slots 

RapidCast Gel Caster, 9 x 11 cm for use with 80-0911- 

EM40-0911-CST 

RCGT 

Allows casting of three gels simultaneously. CASTER 

ONLY 

EM40-0911-GK Gasket, for RapidCast Gel Tray 80-0911-RCGT, pk 2 

Mini L 

Features 






Cat. 

EM40-1214 





• 2 Combs: 1.5 mm thick (12, 20 teeth) 

Description 

Mini L Horizontal 

Accessories 

Cat. 

Description 









EM40-1214-12C 

12 tooth Comb, 1 x microtiter format, 1.0 mm thick, 

Sample volume: 27 µl 

EM40-1214-12D 

12 tooth Comb, 1 x microtiter format, 1.5 mm thick, 

Sample volume: 35 µl 

EM40-1214-25C 

25 tooth (12x2)+1), 2x microtiter format, 1.5 mm 

thick, Sample volume: 11 µl 

EM40-1214-25D 

25 tooth (12x2)+1), 2x microtiter format, 1.0 mm 


EM40-1214-9C 

8+1 tooth Comb, 1 x microtiter format, 1.0 mm 


EM40-1214-9D 

8+1 tooth Comb, 1 x microtiter format, 1.5 mm 


EM40-1214-PD 

2 well, Preparative Comb, 1.5 mm thick, Sample 

volume: 596/28 µl 

EM40-1214-WC Wall Comb, 1.5 mm thick 

EM40-1214-GK Gasket, for RapidCast UVT Tray 80-1214-RCGT, pk. 2 

EM40-1214-UVT12 

RapidCast UVT Gel Tray, 12 x 14 for 80-1214 with 

twelve Comb slots 

EM40-1214-UVT4 

RapidCast UVT Gel Tray, 12 x 14 for 80-1214 with 

four Comb slots 

RapidCast Gel Caster 12 x 14 cm for use with 80- 

EM40-1214-CST 

1214-RCGT 

Allows for three gels to be cast simultaneously. 

CASTER ONLY 

21


Mini ExM 

Features 

Gel Size 14.4 x 10.2 cm 





Cat. 

EM40-1410 

Description 

Mini ExM Horizontal 

Accessories: 

A wide range of Combs 

(compatible with multichannel 

pipettors) are available. Please 

contact EuroClone for the 

complete list of accessories. 


• Casting Chamber 




• 4 Combs: 1.5 mm thickness (2 x 30, 2 x 40 teeth) 

Mini ExW 

Features 






Cat. 

EM40-2314 

Description 

Mini ExW Horizontal 

Accessories: 

A wide range of Combs (compatible with multichannel pipettors) are 

available. Please contact EuroClone for the complete list of accessories. 

Maxi L system allows 40 cm long runs). 



• SuperSafe with Attached Leads, Casting Chamber 


• 4 Combs: 1.5 mm thickness (50 teeth microtiter format) 

Maxigel Systems 

Maxi Gel Systems are very high quality electrophoresis systems designed 

for medium to high sample throughput (up to 600 samples can be 

run simultaneously) and for high-resolution nucleic acid separations 

requiring long migration paths (the Maxi L system allows 40 cm long 

runs). 

Maxi S 

Features 


Footprint 10 x 21.5 x 37.5 cm 




Cat. 

EM41-1325 

Description 

Maxi S Horizontal 




• UV-Transmissible (UVT) Gel Tray with Endgates 

• Three Point Leveling Base 

• 3 Combs: 1.5 mm thickness (12, 16, 20 teeth) 

Accessories: 

A wide range of Combs (compatible with multichannel pipettors) are 

available. Please contact EuroClone for the complete list of accessories. 

22


Maxi M 

Features 


Footprint 29 x 10 x 37 cm 


Buffer Volume max. 2300 ml 


Cat. 

EM41-2025 

Description 

Maxi M Horizontal 






• 3 Combs: 1.5 mm thickness (16, 24, 36 teeth) 

Accessories: 

Cat. 

Description 

EM41-2025-BP 

Long Horizontal Gel Box, 20 x 25 cm with integral leveling platform & Buffer Exchange Ports; 

ExpressCast Gel Tray with six Comb slots and three Combs 

EM41-2025-EG 

End gates for ExpressCast UVT Gel Tray 81-2025-EXGT, pk 2 includes gaskets 

EM41-2025-GK Gasket, for ExpressCast UVT Gel Tray 81-2025-EXGT, pk 2 

EM41-2025-UVT 

ExpressCast UVT Gel Tray 20 x 25 cm with gasketed end gates and six Comb slots that allows 

for 1, 2, 3 or 4 gels of equal length to be run in the tray 

EM41-2025-12C 12 tooth Comb 1.0 mm thick, Sample volume: 54 µl 

EM41-2025-12D 12 tooth Comb 1.5 mm thick, Sample volume: 82 µl 

EM41-2025-16C 16 tooth Comb 1.0 mm thick, Sample volume: 39 µl 

EM41-2025-16D 16 tooth Comb 1.5 mm thick, Sample volume: 59 µl 













EM41-2025-18C 18 tooth (16 sample lanes & 2 marker lanes) 1 x microtiter Comb, 1.0 mm thick, Sample volume: 27 µl 

EM41-2025-18D 18 tooth (16 sample lanes & 2 marker lanes) 1 x microtiter Comb 1.5 mm thick, Sample volume: 40 µl 

EM41-2025-MTC 21 tooth 1 x microtiter Comb, 1.0 mm thick, Sample volume: 27 µl 

EM41-2025-MTD 21 tooth 1 x microtiter Comb, 1.5 mm thick, Sample volume: 40 µl 

EM41-2025-MT2C 42 tooth 2 x microtiter Comb, 1.0 mm thick, Sample volume: 11 µl 

EM41-2025-MT2D 42 tooth 2 x microtiter Comb, 1.5 mm thick, Sample volume: 16 µl 

EM41-2025-PD Preparative Comb, 1.5 mm thick, Sample volume: 1052/28 µl 

EM41-2025-WC 

Wall Comb, 1.5 mm thick 

Maxi L 

Features 


Footprint 32 x 10 x 53 cm 



Gel Volumne 460 ml 

Cat. 

EM41-2340 

Maxi ExW 

Features 

Description 

Maxi L Horizontal 


Footprint (W x H x L) 30 x 11 x 37 cm 


Buffer Capacity max. 3000 ml 


Cat. 

EM41-2325 

Description 

Maxi ExW Horizontal 



• SuperSafe with Attached Leads 




Accessories* 


• Buffer Chamber 





Accessories* 

*A wide range of Combs (compatible with multichannel pipettors) are available. Please contact EuroClone for the complete list of accessories. 

23

3. Nucleic Acids Electrophoresis - Euroclone

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