Molecular Approach to Identify Source of Fecal Contamination EPA ...

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• Rural farm community Last known major crime: Surface Water Contamination Walkerton, Ontario • The well water was contaminated by surface water carrying livestock waste immediately after heavy rain storms. • The drinking water was insufficiently chlorinated to kill E. coli from the manure, causing the outbreak. • A virulent strain of intestinal bacteria left 7 dead and thousands ill. Phenotypic and molecular typing methods confirmed the presence of a single E. coli type - O157:H7 and a variety of Campylobacter spp. strains, which were also found in cattle from nearby farms.
How do we search for pathogens By using indicator organisms: • Relatively easy to isolate. • Survive longer that most disease- producing organisms. • Are almost always present in water containing enteric pathogens.
Page 1 and 2: The Application of a DNA-based Mole
Page 3 and 4: Why are we interested in these guys
Page 5 and 6: Known Hang-outs Primary Sources: Ag
Page 7: Modus Operandi (MO) Usually enter s
Page 11 and 12: Known Associates • Fecal Coliform
Page 13 and 14: How do we address this issue By exp
Page 15 and 16: Bacterial Source Tracking Types of
Page 17 and 18: Which indicator will we go after E.
Page 19 and 20: The Capture and Isolation of Entero
Page 21 and 22: PCR Cycling Reactions • Inoculate
Page 23 and 24: Polymerase Chain Reaction
Page 25 and 26: Visualization of DNA Product Lane 1
Page 27 and 28: Ongoing Related Work • The staff
Page 29 and 30: Resource Documents • Ambient Wate
Page 31 and 32: Acknowledgements • Dr. Marirosa M

Molecular Approach to Identify Source of Fecal Contamination EPA ...

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