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Molecular Approach to Identify Source of Fecal Contamination EPA ...

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• Rural farm community<br />

Last known major crime:<br />

Surface Water <strong>Contamination</strong><br />

Walker<strong>to</strong>n, Ontario<br />

• The well water was contaminated by surface water carrying<br />

lives<strong>to</strong>ck waste immediately after heavy rain s<strong>to</strong>rms.<br />

• The drinking water was insufficiently chlorinated <strong>to</strong> kill E. coli<br />

from the manure, causing the outbreak.<br />

• A virulent strain <strong>of</strong> intestinal bacteria left 7 dead and<br />

thousands ill. Phenotypic and molecular typing methods<br />

confirmed the presence <strong>of</strong> a single E. coli type - O157:H7<br />

and a variety <strong>of</strong> Campylobacter spp. strains, which were also<br />

found in cattle from nearby farms.

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