Molecular Approach to Identify Source of Fecal Contamination EPA ...

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Ongoing Related Work • The Homeland Security, Biological Methods Review Subteam, with contrac<strong>to</strong>r support, will publish a document which summarizes methods recommended for detecting biological agents. PCR, , with culture techniques, is the single, most prevalent method for detecting agents <strong>of</strong> concern.
Resource Documents • Ambient Water Quality for Bacteria – 1986 • National Recommended Water Quality Criteria-Correction Correction – 1999 • Implementation Guidance for Ambient Water Quality Criteria for Bacteria-2002 • The above documents can be found at http://www.epa.gov/waterscience/humanhealth/microbial/mi crobial.html
Page 1 and 2: The Application of a DNA-based Mole
Page 3 and 4: Why are we interested in these guys
Page 5 and 6: Known Hang-outs Primary Sources: Ag
Page 7 and 8: Modus Operandi (MO) Usually enter s
Page 9 and 10: How do we search for pathogens By u
Page 11 and 12: Known Associates • Fecal Coliform
Page 13 and 14: How do we address this issue By exp
Page 15 and 16: Bacterial Source Tracking Types of
Page 17 and 18: Which indicator will we go after E.
Page 19 and 20: The Capture and Isolation of Entero
Page 21 and 22: PCR Cycling Reactions • Inoculate
Page 23 and 24: Polymerase Chain Reaction
Page 25 and 26: Visualization of DNA Product Lane 1
Page 27: Ongoing Related Work • The staff
Page 31 and 32: Acknowledgements • Dr. Marirosa M

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