Molecular Approach to Identify Source of Fecal Contamination EPA ...

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• Verify enterococci On the Road <strong>to</strong> PCR • Subculture isolates for purity • Inoculate the template or vials with sample • Prepare master mix- unique and specific primers
PCR Cycling Reactions • Inoculate the template with master mix • Load the template in<strong>to</strong> the Thermal Cycler • A typical run takes 2 hours
Page 1 and 2: The Application of a DNA-based Mole
Page 3 and 4: Why are we interested in these guys
Page 5 and 6: Known Hang-outs Primary Sources: Ag
Page 7 and 8: Modus Operandi (MO) Usually enter s
Page 9 and 10: How do we search for pathogens By u
Page 11 and 12: Known Associates • Fecal Coliform
Page 13 and 14: How do we address this issue By exp
Page 15 and 16: Bacterial Source Tracking Types of
Page 17 and 18: Which indicator will we go after E.
Page 19: The Capture and Isolation of Entero
Page 23 and 24: Polymerase Chain Reaction
Page 25 and 26: Visualization of DNA Product Lane 1
Page 27 and 28: Ongoing Related Work • The staff
Page 29 and 30: Resource Documents • Ambient Wate
Page 31 and 32: Acknowledgements • Dr. Marirosa M

Molecular Approach to Identify Source of Fecal Contamination EPA ...

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