Molecular Approach to Identify Source of Fecal Contamination EPA ...

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Bacterial Source Tracking • Develop a library or database of isolates taken from known sources (e.g. human, cow, deer, etc.). • The size of the library will be partly determined by the number of potential major sources of fecal pollution in the target area.
Which indicator will we go after E. coli or enterococci • persists longer in the environment than E. coli • can survive in adverse conditions (salt water conditions) • species are more source specific than E. coli • found in 80-90% of clinical isolates taken from humans with infections
Page 1 and 2: The Application of a DNA-based Mole
Page 3 and 4: Why are we interested in these guys
Page 5 and 6: Known Hang-outs Primary Sources: Ag
Page 7 and 8: Modus Operandi (MO) Usually enter s
Page 9 and 10: How do we search for pathogens By u
Page 11 and 12: Known Associates • Fecal Coliform
Page 13 and 14: How do we address this issue By exp
Page 15: Bacterial Source Tracking Types of
Page 19 and 20: The Capture and Isolation of Entero
Page 21 and 22: PCR Cycling Reactions • Inoculate
Page 23 and 24: Polymerase Chain Reaction
Page 25 and 26: Visualization of DNA Product Lane 1
Page 27 and 28: Ongoing Related Work • The staff
Page 29 and 30: Resource Documents • Ambient Wate
Page 31 and 32: Acknowledgements • Dr. Marirosa M

Molecular Approach to Identify Source of Fecal Contamination EPA ...

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