Molecular Approach to Identify Source of Fecal Contamination EPA ...

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The Usual Suspects • Total Coliform- primarily used to assess potable water acceptability. Last known place of residence: naturally occurs and can be found in plants and soils. >1000 cfu/100mL total coliform and 33 cfu/100mL E. coli
Known Associates • Fecal Coliform- primarily used to assess wastewater and other non-potable water quality. Last known place of residence: gastrointestinal tract of humans and warm-blooded animals. • E. coli- recommended to assess quality of fresh water. Last known place of residence: gastrointestinal tract of humans and warm-blooded animals. • Enterococci- recommended to assess quality of marine water. Last known place of residence: gastrointestinal tract of humans and warm-blooded animals.
Page 1 and 2: The Application of a DNA-based Mole
Page 3 and 4: Why are we interested in these guys
Page 5 and 6: Known Hang-outs Primary Sources: Ag
Page 7 and 8: Modus Operandi (MO) Usually enter s
Page 9: How do we search for pathogens By u
Page 13 and 14: How do we address this issue By exp
Page 15 and 16: Bacterial Source Tracking Types of
Page 17 and 18: Which indicator will we go after E.
Page 19 and 20: The Capture and Isolation of Entero
Page 21 and 22: PCR Cycling Reactions • Inoculate
Page 23 and 24: Polymerase Chain Reaction
Page 25 and 26: Visualization of DNA Product Lane 1
Page 27 and 28: Ongoing Related Work • The staff
Page 29 and 30: Resource Documents • Ambient Wate
Page 31 and 32: Acknowledgements • Dr. Marirosa M

Molecular Approach to Identify Source of Fecal Contamination EPA ...

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