CURRICULUM VITAE - University of Ottawa Heart Institute

CURRICULUM VITAE
Department: Pathology and Laboratory Medicine, and
Biochemistry, Microbiology and Immunology 31 January 2008
Name:
Kuroski de Bold, Mercedes Lina Employee No: 3364
Assistant Professor
Gender: Female
DEGREES:
Ph.D., Experimental Pathology, Queen’s University, Kingston, On., 1974
M.Sc., Experimental Pathology, Queen’s University, Kingston, On., 1972
B.Sc. Laboratory Medicine, University of Cordoba, Cordoba, Argentina, 1968
EMPLOYMENT HISTORY:
1999- Cross-appointed, Department of Biochemistry, Microbiology and Immunology
1986- Assistant Professor, Department of Pathology and Laboratory Medicine, Faculty
of Medicine, University of Ottawa, Ottawa, On.
1985-86 Assistant Professor (Adjunct), Dept. of Pathology , Queen’s University, Kingston,
On.
1978-85 Research Associate, Department of Pathology, Queen’s University, Kingston,
On.
1970-74 Instructor, Department of Pathology, Queen’s University, Kingston, On.
1970-72 Instructor, Department of Pathology, Queen’s University, Kingston, On.
1963-68 Teaching Assistant, Department of Pathology, University of Cordoba, Cordoba,
Argentina
DISTINCTIONS
Listed in American Men and Women of Science, 1986-
SCHOLARLY AND PROFESSIONAL ACTIVITIES:
INTRAMURAL ACTIVITIES:
2006- present Problem Based Learning Tutor, (facilitator) Human Reproduction and Sexuality
Block, Med 2102, (2 nd year medical students). Five weeks, 40 h.
1992-present Problem Based Learning Tutor, (facilitator) Gastrointestinal Block, Med 2107, (2 nd
year medical students). Six weeks, 50 h
1

2005- present Co-Director, Pathology for graduate students, CMM 5001
2004- present Facilitator, Problem Based Learning Tutor Training Workshops. Three times/year, 8
h/session.
2004- present Executive Member, Problem Based Learning Tutor Training Committee
2004-2007 Co-Director Core Pathology Laboratory, University of Ottawa Heart Institute
2003-2004 Planning and Design Committee, Core Pathology Laboratory, University of Ottawa
Heart Institute
2003- present Abstract Reviewer, Canadian Cardiovascular Society
2000 World Artificial-Organ, Immunology and Transplantation Society
Member, International Scientific Committee, 6 th Symposium, Ottawa,
August 17-20, 2001
2000- Basic Science Judge, University of Ottawa Heart Institute Research Day April 11 th
2000- Executive Organizing Committee, ANF 2000 Symposium, ANatriuretic Peptides and
Vasopeptidase Inhibitors@, Aug 18-19, Satellite of the 18 th International
Hypertension Society meeting
2000-present Organizer, FRONTIERS IN HEART FAILURE RESEARCH DISTINGUISHED
VISITORS SEMINARS SERIES, University of Ottawa Heart Institute Sponsor by
Merck Frosst Canada and the Cardiovascular Endocrinology Heart Failure Research
Group. This is an accredited Group Learning Activity as defined by the RCPSC.
8 h/month
2000-2001 University of Ottawa Animal Care Committee (ACC), Acting Chair. Duties;
Monthly meetings; preparing agendas and minutes of meetings; revision of submitted
protocols; letter writing advising investigators of ACC Protocol Review group (PRG)
decisions; contacting investigators personally to answer queries from ACCPRG;
giving executive approval to protocols; consultant for Director of ACVS at the
university regarding ethical animal issues; site visits to animal facilities; training on
animal care for new personnel according to CCAC guidelines; updated
documentation for Council on Animal Care (CCAC) assessment visit; responded to
2

assessment panel commentaries, recommendations and queries; implementation of in
house peer review process for scientific merit; consultant for Policy Development for
ACC; development of pro-active steps for CCAC compliance; attending executive
meetings with Vice-Rector (Academic) holder of the Ontario license.
Preparation of the Annual Report of the Animal Care Committee for submission to
the Vice-Rector Academic, holder of the Ontario license. Time: 59 +h/m
1999-2005 University of Ottawa Animal Care Committee Editor, Animal Care Committee
Newsletter. 5 h/ week
1998 University of Ottawa Heart Institute Youth Symposium Organizer; Canadian
Medical Hall of Fame-Canada Trust Youth Symposium- October 28-29/1998
delegate
1998-2005 University of Ottawa Heart Institute, Ottawa Animal Care Committee delegate for
Director of Academic Affairs
1996- present University of Ottawa, Vice-Chair, Ottawa Animal Care Committee
1988- present University of Ottawa Heart Institute, Ottawa Animal Care Committee Protocol
Review Group, delegate, 5 h/ month.
1988- present University of Ottawa Heart Institute, Ottawa Animal Care Committee delegate, 4 h,
2 times a year
1993-1995 University of Ottawa Heart Institute Shared Resources Committee/Lab Resources
1993-1994 University of Ottawa Heart Institute Telethon
1992-1998 University of Ottawa, Research Development Committee, Department of Pathology
and Laboratory Medicine
1989-1990 Executive, Organizing Committee, Satellite Symposium of the 8th International
Hypertension Society AA Decade of ANF Research@ Ottawa
1988-1990 University of Ottawa Heart Institute Intensive Care Committee,
1988-1990 University of Ottawa Heart Institute Research Advisory Committee
3

REVIEWER
REVIEWING ACTIVITIES FOR BIOMEDICAL JOURNALS
2001-2008 NUMBEROF ARTICLES REVIEWED
Brain Research Bulletin 1
Cardiovascular Research 19
Canadian Cardiovascular Society
Abstract Reviewer
Cell Physiology and Biochemistry 1
Clinica Quimica Acta 1
Journal of Molecular and Cell Cardiology 1
Maturitas 3
Proceedings of the National Academy of Sciences 1
EXTRAMURAL ACTIVITIES:
2002- present Member, Canadian Cardiovascular Society Membership Committee
2002- present Member, Canadian Council on Animal Care Core Components Working Group
1999- present Editorial Board, Canadian member of Canadian Council on Animal Care on
"Animales de Experimentación ", Mexico , DF., Mexico
1999- present Reviewer Spanish language for “ Manual sobre el cuidado y uso de los animals de
experimentación”
1998- present Sanofi-Aventis Biotechnology Challenge, Resource person
1998- present University of Ottawa Heart Institute Aventis Student Biotechnology Exhibition,
delegate
1997- present Executive Consultant Faculty of Chemical Sciences, UNC, Cordoba, Argentina
1990- present Consultant for the Canadian Council on Animal Care.
4

Teaching Activities:
-High School
-CO-OP Education Program
Feb-June 2009 Phara Desroches-Julien, Franco-Cité Catholic High School
Feb-June 2009 Mustafa Zeybel, Bell High School
Feb-June 2008 A. El-Salfiti, Merivale High School Ottawa, On,
Feb-June 2008 Harshami Dabere, Merivale High School Ottawa, On,
Feb-June 2007 Tom McNeill, All Saints High School, Kanata, On
Jan- Sep 2006 *Niket Gandhi, Bell High School, Bells Corners, Ottawa, On
Feb-June 2005 *Nancy Maltez, Collège Catholique Samuel-Genest ( Secondaire)
Feb-June 2005 Rima Azzi, , Collège Catholique Samuel-Genest
Sep-Jan 2004/05 Jessica Reszel, Holy Trinity High School, Kanata, On
Sep-Jan 2004/05 Nick Wong, Merivale High School, Ottawa, On
Sep-Jan 2004 Bogdan Momciu, Earl of March High School, Kanata, On
Feb-June 2003 *Erin Kelly, Holy Trinity High School, Ottawa, On
Feb-June 2003 Daric Biggs, All Saints Catholic High School, Ottawa, On
Feb-June 2002 *Ania Syrowatka, Holy Trinity High School, Ottawa, On
Feb-June 2002 *Keith Jansa, Mother Theresa High School, Ottawa, On
Feb-June 2001 Maria Zlotorzynska, Merivale High School, Ottawa, On
Sep-Jan 2000/01 Stephanie Chhangur, Merivale High School, Ottawa, On
Sep-Jan 2000/01 Amy Hanlan, Mother Theresa, High School, On
Sep-Jan 1999/00 Nathan Juneau, Holy Trinity High School, Kanata, On
*These students went on to obtain a BSc in Biochemistry.
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UNIVERSITY UNDERGRADUATE
Supervisor Fourth Year Honors Project,
Allison Huckstep, Biochemistry, Microbiology and Immunology. Thesis title,
“ANF-HSA, a fusion protein with natriuretic peptide biological activity.”2008-
2009
Amanda Yueting Cheng, Biochemistry, Microbiology and Immunology. Thesis
title, “Genome Wide Scan of Formalin Fixed Paraffin Embedded Cardiac Graft
Tissue to Study the Coordinate Expression of Cytokines and Brain Natriuretic
Peptide” 2004-2005
Vanja Vukic, Biochemistry, Microbiology and Immunology. Thesis title,
“Pharmacological Properties of CJC-1382, a Long Acting ANF Analog” 2003-
2004
2006-present Problem Based Learning Tutor, (facilitator) Human Reproduction and Sexuality
Block, Med 2102, (2 nd year medical students). Promoting problem solving/critical
thinking, understanding group dynamics, creating a positive learning climate and
able to ask appropriate, stimulating and challenging questions. 5 weeks, 40 h
1992-present Problem Based Learning Tutor, (facilitator) Gastrointestinal Block, Med 2107, (2 nd
year medical students). Promoting problem solving/critical thinking, understanding
group dynamics, creating a positive learning climate and able to ask appropriate,
stimulating and challenging questions. 6 weeks, 50 h.
2004-present Facilitator, Problem Based Learning, Tutor Training Workshops, for member of the
Faculty of Medicine offered by the Faculty Development office. This is an accredited
Group Learning Activity as defined by the RCPSC.
Three times a year, 8 h/ session.
2003 Supervisor, Laura Zadro, BSc. Honours, summer student
2003 Erin Kelly, Grade 12 - Holy Trinity High School,
2003 Supervisor, Ania Syrowatka, BSc. Honours, summer student
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2000 Supervisor, Andrew Leduc Martin L. Wills High School Student Scholarship
2001 Supervisor. Andrew Leduc Ontario Heart and Stroke Foundation J.D.Schultz
Scholarship
1986-1988 André Bédard, B.Sc.(Med), University of Ottawa, Faculty of Medicine. Project
Director. “Cardionatrin Release from the Human Atria@ Medical student Thesis
accomplished in two consecutive summers.
GRADUATE AND POSTGRADUATE
2005-present CMM 5001, Pathology for graduate students, graduate fall course.
Co-Director and teacher (10 h/session) of the course.
2005 Examiner, Steven Theriault, MSc., Thesis. Faculty of Graduate and Postgraduate
Studies, Department of Biochemistry, Microbiology and Immunology, University
of Ottawa
2005 Examiner, Stephanie Dean, MSc., Thesis. Faculty of Graduate and Postgradute
Studies, Stephanie Dean, MSc, Department of Cellular and Molecular Medicine,
Faculty of Medicine, University of Ottawa
2004-present Facilitator, Problem Based Learning, Tutor Training Workshops for member of the
Faculty of Medicine offered by the Faculty Development office. This is an accredited
Group Learning Activity as defined by the RCPSC.
2003-2006 Comprehensive Examiner, PhD Faculty of Graduate and Postgraduate Studies
Mary Anne Kent Department of Biochemistry, Molecular Biology and
Microbiology, Faculty of Medicine, University of Ottawa
1992-present Workshops on Handling of Laboratory Animals , University of Ottawa Heart
Institute 40 h/year
1988-2000 Heart and Stroke Foundation of Ontario Summer Student training.
1987-present Cardiovascular Endocrinology Resource Faculty
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DIRECTED STUDIES
- Allison Huckstep, (BSc Honors), University of Ottawa, expected 2009
-Amanda Y. Cheng. (BSc Honors), University of Ottawa, 2005
-Vania Vukic, BSc (Honors), University of Ottawa, 2004
-Shann Dixon Kim, Ph.D., (Physiol) University of Illinois at Chicago, Medical-Surgical Nursing,
College of Nursing, 1996-99
-Gilles Dubé, M.Sc., (Physiol), University of Ottawa, 1993
-André Bédard, B.Sc. (Med), University of Ottawa, 1992
-Michael Bensimon, M.Sc., (Physiol), 2002
-Ken Ma, PhD (Physiol), 2005
CONTINUING EDUCATION
1998- Managing the Situation. Management workshop. Faculty of Medicine, University of Ottawa.
June 8, 1998
1998- Principles of Project Management@. Queen’s, Ottawa and Carleton Universities, Drs. P.
Bamji and B. Hutchinson instructor. June 8-24, 1998, Ottawa, On
1994 In Situ Hybridization Workshop. Life Technologies Inc. November 15-19, Germantown,
Maryland, USA.
1992 Animal Care Committees; their Role and Responsibilities. Workshop Canadian Council on
Animal Care, Ottawa, February 10-11, 1992
1991 Recent Advances in Molecular Pathology, Tufts University School of Medicine,
April 3-6, Boston , Massachusetts, USA
1988 Advances in Biotechnology Concepts in Molecular Biology. American Association of
Pathologists, October 30-November 2, 1988, Bethesda, Maryland, USA.
1988 UCLA Symposium on Molecular Biology of the Cardiovascular System, April 10-16,
Keystone, Colorado, USA.
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EXTERNAL RESEARCH FUNDING:
Source Type Amount Purpose
CIHR C $145,360 / 07-08 Co-PI Research
CIHR C $485,442 / 06-11 Co-PI Research
ConjuChem O $ 54,000 / 03-04 PI Research
CIHR C $101,699 / 02-05 Co-PI Research
CIHR C $192,168 / 99-03 PI Research
CIHR C $369,440 / 01-05 Co-PI Research
Merck-Frosst O $ 40,000 / 00-present PI Heart Failure Seminars
PUBLICATIONS:
- Books authors 0
- Books edited 0
- Chapters in books 4
- Papers in peer reviewed journals 33
- Papers in non-referred journals 0
- Technical reports 0
- Abstracts and Presentations 40
Guest speaker 14
Patents and Copyrights
de Bold, A.J., Kuroski de Bold, M.L., Sheffield, WP.
Atrial Natriuretic Factor Analogue.
Full patent filed Oct 20, 2006
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Refereed Chapters in Books
“Natriuretic Peptides as Neuromodulators in the Brain” (In Press). The New Encyclopedia of
Neuroscience. Ed. Larry Squire et al, Elsevier, 2008
de Bold, ML., de Bold, AJ
“Immunocytochemical Demonstration of Cardionatrins in Human Myocardium”
de Bold, ML, de Bold, AJ. In: Immunocytochemintry in Tumor Diagnosis. Ed. J. Russo, Martinus
Nijhoff Publishing, Boston Mass., 1985:202-206
“Atrial Natriuretic Factor”
de Bold, AL, de Bold MLK, Ruben, MM. In: Proc. X Intern Cong Nephrolo 1:109-122, 1988 Ed.
Davidson, AM. London, England
“Atrial Natriuretic Factor”,
de Bold AJ, de Bold, MLK, Ruben, MM. In: Prohormones, Hormones and their Fragments:
Processing, Biological Activity and Pharmacology. Ed. Ellis Horwood Ltd. 1989, Chapter 11:317-
124.
Papers in referred Journals
Rabbit Pancreatic Cell. Morphological and Functional Studies During Embryonal and Post-Natal
Development.
Bencosme, S.A., Wilson, M.B., Aleyassine, H., de Bold, A.J., and de Bold, M.L.
Diabetologia, 6:399-411, 1970.
Demonstration of a Tryptaminergic Mechanism in the Rat-Cell.
Pulido, O., de Bold, A.J., de Bold, M.L., and Bencosme, S.A.
Experientia, 33:268, 1977.
Intracellular Pancreatic Cell Serotonin and the Dynamics of Insulin Release.
Pulido, O., Bencosme, S.A., de Bold, M.L., and de Bold, A.J.
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Diabetologia, 15:197-204, 1978.
Effects of Serotonin and its Antagonists on the First Phase of Insulin Release in Normal and
Hypersomatotrophic Rats.
De Bold, M.L., and Bencosme, S. A.
Horm. Metab. Res. 10:500-505,1978.
Structural Relationships Between Parenchymal and Stromal Elements in the Pars Intermedia of the
Rat Adenohypophysis as Demonstrated by Extracellular Space Markers.
de Bold, A.J., de Bold, M.L., and Kraicer, J.
Cell and Tissue Res., 207:347-359, 1980.
The Amino Acid Sequence of an Atrial Peptide with Potent Diuretic and Natriuretic Properties.
Flynn, T.G., de Bold, M.L., and de Bold, A.J.
Biochem. Biophys. Res. Commun., 117:859-865, 1983.
Demonstration of Stellate Cells of the Pars Intermedia of the Pituitary Gland Using a New Silver
Impregnation Technique.
de Bold, M.L., de Bold, A.J. and Kraicer, J.
Stain Technol., 59:49-52, 1984.
Alignment of Rat Cardionatrin Sequences with the cDNA Derived Sequence of Preprocardionatrin.
Flynn, T.G., Davies, P.L., Kennedy, B.P., de Bold, M.L., and de Bold, A.J.
Science, 228:323-325, 1985.
Main Forms of Immunoreactive Cardionatrin in Atrial Extracts and in Atrial Specific Granules.
Flynn, T.G., de Bold, A.J., de Bold, M.L., Davies, P.L., and Kennedy, B.P.
Biochem. Soc. Trans., 13:1141, 1985.
Functional-Morphological Studies on In Vitro Cardionatrin Release.
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de Bold, A.J., de Bold, M.L., and Sarda, I.R.
J. Hypertension, 4: S3-S7, 1986.
Factors Affecting the Release of Immunoreactive Cardionatrin (IRC) In Vitro from Human and Rat
Atria.
de Bold, A.J., de Bold, M.L., Taichman, G.C., Boyd, D.A. and Keon, W.J.
The Second World Congress on Biologically Active Atrial Peptides, New York, N.Y., May 20-
21,1987.
Effect of Manipulations of Ca 2+ Environment on Atrial Natriuretic Factor Release.
de Bold, M.L. and de Bold, A.J.
Am. J. Physiol., 256: H1588-H1594, 1989.
Optimization of Atrial Natriuretic Factor Radioimmunoassay.
Sarda, I.R., de Bold, M.L., and de Bold, A.J.
Clin. Biochem., 22: 11-15, 1989.
Stretch-Secretion Coupling in Atrial Cardiocytes: Dissociation Between ANF Release and Atrial
Contractility
Kuroski de Bold, M.L., and de Bold, A.J.
Hypertension, 18, (Suppl. III):III-169 - III-178, 1991.
A Decade of ANF Research.
de Bold, A.J., Kuroski de Bold, M.L., Boer P.H., Mangat, H., Dubé, G., Johnson, F.
Can. J. Physiol. Pharmacol., 69, (10) 1480-1485, 1991
Post-transcriptional Processing of Atrial Natriuretic Factor by Adult Rat Atrial Cardiocytes in
Culture.
Dubé, G.R., Kuroski de Bold, M.L., and de Bold, A.J.
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Can. J. Physiol. Pharmacol., 69: 1480-1485, 1993
Epicardial Mesothelial Cells Synthesize and Release Endothelin.
Eid, H., Kuroski de Bold, M.L., Chen, J.H. and de Bold, A.J.
J. Cardiovasc. Pharmacol., 24:715-720, 1994
Atrial Natriuretic Factor Significantly Contributes To The Mineralocorticoid Escape Phenomenon:
Evidence For A Guanylate Cyclase-Mediated Pathway.
Yokota, N., Bruneau, B., Kuroski de Bold, M.L., and de Bold, A.J.
J. Clin. Invest., 94:1938-1946, 1994.
Dissociation of Cardiac Hypertrophy, Myosin Heavy Chain Isoform Expression, and Natriuretic
Peptide Production in DOCA-Salt Rats.
Yokota, N., Bruneau, G.G., Fernandez, B.E., Kuroski de Bold, M.L., Piazza, L.A., Eid, H., and de
Bold, A.J.
Am. J. Hypertens., 8:301-310, 1995
Mechanical and Neuroendocrine Regulation of the Endocrine Heart
de Bold, A.J., Bruneau, B., Kuroski de Bold, M. L.
J. Cardiovasc. Res. 31:7-18, 1996
Evidence for Load-Dependent and Load-Independent determinants of Cardiac Natriuretic Peptide
Production in Hypertensive Rats with or without cardiac hypertrophy
Ogawa, T., Linz, W., Stevenson, M., B. Bruneau, Kuroski de Bold, M.L., H. Eid., Schölkens and de
Bold, A.
Circulation, 93:2059-2067, 1996
Tissue Specific Regulation of Renal and Cardiac Natriuretic Factor Gene Expression in DOCA-Salt
Rats
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Ogawa, T., Bruneau, B.G., Yokota, N., Kuroski de Bold, M.L., de Bold A.J.
Hypertension 30:1342-1347, 1997
Atrial Natriuretic Factor and Brain Natriuretic Peptide Gene Expression in the Spontaneously
Hypertensive Rat During Postnatal Development
Kuroski de Bold M.L.,
Am J Hypertens 11(8):1006-1018, 1998
Estrogen, Natriuretic Peptides and the Renin-Angiotensin System
Kuroski de Bold, M.L.
Cardiovasc Res 41:524-531, 1999
Cardiomyopathy in Irx-4 Deficient Mice is Preceded by Abnormal Ventricular Expression
B.G. Bruneau, Zheng-Z. Bao, Fatkin D, Xavier-Neto, J, Georgakopoulos D, MacGuire C.T., Berul.
C.I., Kass, DA, Kuroski de Bold, M.L., de Bold, A.J., Conner, DA, Rosenthal, N, Cepto,CL,
Seidman JG and Seidman CE.
Mol Cell Biol 21(5):1730-1736, 2001
Estrogen Modulation of Brain Natriuretic Peptide Storage and Release
Kuroski de Bold, Mercedes L.
Faseb J 15(5) Pt II : A774, 2001
The Physiological and Pathophysiological Modulation of the Endocrine Function of the Heart.
DeBold, AJ, Ma, K, Zhang,Y, Kuroski de Bold, ML Bensimon, M , Koshbatten, A.,
Can J Physiol Pharmacol 79:705-714, 2001
Gene Expression of Natriuretic Peptides (NPs) and their Receptors (NPRs) in Human Coronary
Arteries.
Casco, VH, J.P Veinot, ML Kuroski de Bold, R Masters, MA Stevenson, AJ de Bold
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J Histochem Cytochem 50:799-809, 2002
Neuroendocrine Profiling in Humans Receiving Cardiac Allografts
Ogawa, T., Veinot, J.P., Davies, R.A., Haddad, H., Smith, S.J., Masters, R.G., Hendry, P.J., Starling,
R., Kuroski de Bold, M.L., Ponce, A., Ma, K.K., Williams, K.,de Bold, A.J.
J Heart Lung Transplantation , 24(8):1046-54, 2004
Participation of G Proteins in Natriuretic Peptide Hormone Secretion from Heart Atria
Bensimon, M, Chang, AI, Kuroski de Bold, ML, Ponce, M, Carreras, D, de Bold, AJ
Endocrinology 145(11):5313-21, 2004
Determinants of Natriuretic Peptide Production by the Heart: Basic and Clinical Implications.
de Bold, A. J., de Bold MLK
J Investig Med. 53(7):371-7, 2005.
The Endocrine Function of the Heart
McGrath, M.F., de Bold K, M.L., de Bold, A. J.
Trends Endocrinol Metab 2005 Dec: 16(10):469-77. Epub 2005 Nov 2
Relationship Between Natriuretic Peptides and Inflammation: Proteomic Evidence Obtained
During Acute Cellular Cardiac Allograft Rejection in Humans.
Meirovich YF, Veinot JP, de Bold MLK, Haddad H, Davies RA, Masters RG, Hendry PJ, de
Bold AJ.
J Heart Lung Transplant. 2008 Jan; 27(1):31-7.
15

PAPERS IN REFERRED CONFERENCE PROCEEDINGS
Pancreatic Islet Cell Tumor Associated with Hyperinsulinism. Diagnostic Problems.
Bencosme, S.A., Wilson, M.B., Aleyassine, H., de Bold, A.J., and de Bold, M.L.
Annual Congress Quebec Association Lab. Physicians, 1971.
An Improved Method for Total Body Fixation by Intravascular Perfusion.
de Bold, M.L., Feria-Velasco, A., Bencosme, S.A., and de Bold, A.J.
Soc. Exptl. Biol. Med., (Champlain Section), May 1973.
Demonstration of Tryptaminergic Mechanism in the Rat Cell.
Pulido, O.M., de Bold, A.J., de Bold, M.L., and Bencosme, S.A.
Soc. Exptl. Biol. Med. (Champlain Section), May 1976.
Demonstration and Microanatomical Distribution of Stellate Cells of the Pars Intermedia (PI) of the
Rat Pituitary Using a Novel Silver Impregnation Technique.
de Bold, M.L., de Bold, A.J., and Kraicer J.
Proc. Microscop. Soc. Can., 8:80-81, 1981.
Ultrastructure of the Innervation of the Pars Intermedia (PI) of the Rat Pituitary after Administration
of 5-Hydroxydopamine.
de Bold, M.L., de Bold, A.J., and Kraicer, J.
Proc. Can. Fed. Biol. Soc., 24:231, 1981.
Main Forms of Immunoreactive Natriuretic Factor in Atrial Tissue and in Isolated Specific Atrial
Granules.
Davies, P.L., de Bold, A.J., de Bold, M.L., Flynn, T.G. and Kennedy, B.P.
Can. Fed. Biol. Societies, Toronto, Ontario, June 17-21, 1985.
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Different Gene Activation Mode for Atrial Natriuretic Factor versus Structural Heart Muscle Gene.
Boer, P.H., Kuroski-de Bold, M.L., de Bold, A.J.,
Ottawa Symposium on Atrial Natriuretic Factor, "A Decade on ANF Release"
International Hypertension Society Satellite Symposium, Ottawa, June 21-23, 1990
Effect of Angiotensin II, Phorbol Ester and Potassium Depolarization on ANF Release
Kuroski-de Bold, M.L., de Bold, A.J.,
Ottawa Symposium on Atrial Natriuretic Factor, "A Decade of ANF Release"
International Hypertension Society Satellite Symposium, Ottawa, June 21-23, 1990, p 14
Morphological and Functional Studies on the Cell of the Embryonal Pancreas in the Rabbit.
de Bold, A.J., Bencosme, S.A., Wilson, M.B., Aleyassine, H., and de Bold, M.L.
Anat. Rec., 166:296, 1970.
Vascular Perfusion Fixation for Electron Microscopy: An Improved Method.
de Bold, M.L., Feria-Velasco, A., Bencosme, S.A., and de Bold, A.J.
Lab. Invest., 28:382, 1973.
Intracellular-Cell Serotonin and the Dynamics of Insulin Release.
Pulido, O.M., Bencosme, S.A., de Bold, M.L., and de Bold, A.J.
Lab. Invest., 36:348, 1977.
Demonstration of Stellate-Shaped Cells in the Pars Intermedia (PI) of the Rat Hypophysis.
de Bold, A.J., de Bold, M.L., Ludwin, S.K., and Kraicer, J.
Clin. Res., 28:669A, 1980.
Purification and Characterization of Rat Atrial Cardionatrins - Potential Diuretic, Natriuretic and
Vasoactive Hormones.
Flynn, T.G., de Bold, M.L.K and de Bold, A.J.
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Proceedings of the 7th International Congress of Endocrinology. Excerpts Medica International
Congress Series 652. Elsevier Science Publishing Co. Inc., New York, N.Y., pp 873, 1984.
Main Forms of Immunoreactive Natriuretic Factor in Atrial Tissue and in Isolated Specific Atrial
Granules.
Flynn, T.G., de Bold, A.J., de Bold, M.L., Davies, P.L. and Kennedy, B.P.
Biochemical Society Meeting, Cardiff, Wales, March 20-22, 1985.
Atrial Stretch-Secretion Coupling and Immunoreactive Cardionatrin (IRC) Release In Vitro: Effect
of Ca 2+ and EGTA.
de Bold, M.L. and de Bold, A.J.
The First World Congress on Biologically Active Atrial Peptides, New York, N.Y., May
29-June 1, 1986.
Factors Affecting the Release of Immunoreactive Cardionatrin (IRC) In Vitro from Human and Rat
Atria.
de Bold, A.J., de Bold, M.L., Taichman, G.C., Boyd, D.A. and Keon, W.J.
The American Society of Hypertension Second World Congress on Biologically Active Atrial
Peptides, New York, N.Y., May 17-21, 1987.
Factors Affecting Cardionatrin Release.
de Bold, A.J. and de Bold, M.L.
First European Congress of Endocrinology, Copenhagen, Denmark, June 21-25, 1987.
Functional Morphology of ANF Release.
de Bold, A.J. and de Bold, M.L.
UCLA Symposium: Biological and Molecular Aspects of Atrial Factors, Steamboat Springs,
Colorado, January 17-23, 1988.
Effects of Divalent Cations on Lanthanum on Immunoreactive Cardionatrin (IRC; ANF 99-126)
Research.
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de Bold, M.L., and de Bold, A.J.
FASEB J., 2:65, 1988.
Studies on Stretch-Secretion Coupling and Other Cell Properties of Atrial Cardiocytes.
de Bold, A.J., and de Bold, M.L.
Kyoto Symposium on ANP, Kyoto, Japan, July 15-23, 1988.
The Effect of Catecholamines and Neurotransmitters on ANF Release.
de Bold, M.L., and de Bold, A.J.
73rd Annual Meeting Federation of American Societies for Experimental Biology,
New Orleans, Louisiana, March 19-23, 1989.
Studies On In Vivo and In Vitro ANF Production.
de Bold, A.J., and de Bold, M.L.
XXXI International Congress of Physiological Sciences, Helsinki, Finland, July 9-14, 1989.
Endothelin (ET) - Induced Atrial Natriuretic Factor (ANF) Release: Modulation by Calcium
de Bold, A.J., and Kuroski-de Bold, M.L.,
Faseb J. Part I: A289, 1990
Studies on Basal and Stretch-Induced Atrial Natriuretic Factor (ANF) Release
Kuroski-de Bold, M.L., de Bold, A.J.,
Faseb J. Part I: A289, 1990
Presence of Stretch-Activated Channels in Freshly Isolated Adult Rat Heart Cells.
Bustamante, J.O., de Bold M.L., Boer P.H., and de Bold A.J.
Proc. 1991 USCD Asilomar Conference "Molecular and Cellular Biology of the Cardiac Myocyte"
J Cell Biochem Suppl 15C:167, 1991
19

Studies on ANF Processing and Release from Adult Rat Cardiocytes on Microcarried Beads.
G.R. Dubé, M.L. Kuroski de Bold, A.J. de Bold.
FASEB J Pt. II:A1021,1991
Different Gene Activation Mode for Atrial Natriuretic Factor versus Structural Heart Muscle Genes.
Boer, P.H., Kuroski de Bold, M.L., de Bold, A.J.
Proc. 1991 USCD Asilomar Conference "Molecular and Cellular Biology of the Cardiac Myocyte"
J.Cell.Biochem. Suppl 15C: 160, 1991
Activation Mode of the Atrial Natriuretic Factor Gene During P19 Cardiac Myogenesis.
Boer, P.H., de Bold, A.J. Kuroski de Bold, M.L., , Bustamante, J.O.
FASEB J Pt. III:A1748,1991
Natriuretic Peptide Storage and Release by Atrial Cardiomyocytes.
Kuroski-de Bold, M.L., and de Bold, A.J.
Proc. 1991 USCD Asilomar Conference "Molecular and Cellular Biology of the Cardiac Myocyte"
p.042, 1991
Morphological and Biochemical Studies on the Subcellular distribution of A- and B- Type
Natriuretic Factors in the Rat Heart.
Kuroski-de Bold, M.L., Pulido, O., Fernandez, B., and de Bold, A.J.
Faseb J Pt.I: A1234, 1992
Temporal Pattern of Expression of Myosin Heavy Chain, ANF and BNF in Monoculture
and Co-Culture of Adult Rat Ventricular Myocytes with Epicardial Mesothelial Cells.
Eid, H., Chen, J.H., Kuroski-de Bold, M.L. and de Bold, A.J.
J.Cell Biochem. Supp.17D: 210, 1993
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Transcriptional and Post-translational Regulation of Atrial Natriuretic Factor (ANF) and Brain
Natriuretic Peptide (BNP) During the Development of DOCA-Salt Hypertension.
Yokota, N., de Bold, M.L.K., de Bold, A.J.
Circulation 88(4), Pt II, I-315, 1993
Effects of H-142-1 (HS), an Antagonist of Natriuretic Peptide (NP) Bioactive Receptors, on
Deoxycorticosterone Acetate (DOCA) Escape.
Yokota, N., de Bold, M.L.K., de Bold, A.J.
J Am. Soc. Neph. 4(3): 450, 1993
Atrial Natriuretic Factor (ANF) and Brain Natriuretic Peptide (BNP) Production in DOCA-Salt
Hypertension
Yokota, N., B. Bruneau, B. Fernandez, M. K. de Bold, L. Piazza, H. Eid, and A.J. de Bold
J. Mol. Cell Cardiol. 27(6): A 218, 1994
Natriuretic Peptide Production in Hypertensive Rats with or without Cardiac Hypertrophy
Ogawa, T., Linz, W, Stevenson, M., Bruneau, B., Kuroski de Bold, M.L., Chen J.H., Eid, H.,
Schölkens, B.A., de Bold, A.J.
J. Mol. Cell Cardiol. 27(6): A219, 1995
Characterization of the Cardiac Conducting System in the Rat and Monkey (Macaca fascicularis) by
Immunohistochemistry.
Mueller, R., Pulido, O., Kuroski de Bold, M.L. Luus, L., Rowsell, P., Smyth, P., Langlois, I., de
Bold, A.J.
FASEB J. Pt I 9(3): A374, 1995
21

Regulation of Brain Natriuretic Peptide ( BNP) in Male and Female Spontaneously Hypertensive
Rats.
Kuroski de Bold, M.L., de Bold, A.J.
FASEB J Pt. I 9(3): A78, 1995
Natriuretic Peptide Production in Hypertensive Rats with or without Cardiac Hypertrophy
Ogawa, T., W. Linz, M. Stevenson, B. Bruneau, M.L.K. de Bold, J.H.Chen, H. Eid, B.A.
Schölkens and A.J. de Bold
Can. J. Cardiol. 11(Suppl. E): A 128E, 1995
Natriuretic Peptide Production in Hypertensive Rats with or without Cardiac
Hypertrophy
Ogawa, T., W. Linz, M. Stevenson, B. Bruneau, M.L.K. de Bold, J.H.Chen, H. Eid, B.A.
Schölkens and A.J. de Bold
Am. J. Hyperten 9(4; Pt 2):8A, 1996
Pharmacological Properties of CJC-1382, a Long Acting ANF Analog
Kuroski de Bold, ML, Denholm, E, Vukiv,V, Shoushtarian, A, Leger, R, Bridon, D, de Bold, AJ
FASEB J 18(4):A687, Suppl., S March 2004
Properties of CJC -1382, a Long Acting ANF Analog
Kuroski de Bold, ML, Vukiv,V, Denholm, E, Shoushtarian, A, Leger, R, Bridon, D, de Bold, AJ
Canadian Cardiovascular Congress, Calgary , AB, October 2004
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INVITED LECTURES
Gender-related differences in natriuretic peptide gene expression in hypertension
Kuroski de Bold, Mercedes L., de Bold, Adolfo J.
International Society for Heart Research, June 9-13, Chicago, IL, 1996.
J Mol Cell Cardiol 28(6): A172,1996
Blood Pressure and the Female Heart
Kuroski de Bold, M. L
Annual Volunteer Night, Gloucester-Cumberland Chapter, Heart and Stroke Foundation
of Ontario, Ottawa, On. June 20, 1996
Ètica y Normas de la Experimentaciòn Animal: Experiencia Canadiense
Kuroski de Bold, M.L.
Facultad de Ciencias Exactas y Naturales
Universidad de Buenos Aires, Buenos Aires, Argentina. October 10,1996
Ètica y Normas de la Experimentaciòn Animal: Experiencia Canadiense
Kuroski de Bold, M.L.
Facultad de Farmacia y Bioquímica
Universidad de Buenos Aires, Buenos Aires, Argentina. October 11, 1996
Gender- related differences in ANF and BNP gene expression in hypertension
Kuroski de Bold, M L.
Natriuretic Peptides and Vasopressin Inhibitors, International Society of Hypertension Satellite
Symposium, Aug 18-19, 2000
Gender- Related Differences in ANF and BNP Gene Expression in Hypertension
Kuroski de Bold, M L.
23

Natriuretic Peptides and Vasopeptidase Inhibitors@, ANF 2000 Symposium, Satellite of the 18 th
International Hypertension Society meeting. Aug 18-19, 2000
The University of Ottawa Animal Care Committee: Education and Training Program
Session: Research Partnerships- Resolving a Common Need: Implementing an Education and
Training Program
CALAS/ACSAL Symposium June 25-28, 2000
Kuroski de Bold, M. L
Estrogen modulation of brain natriuretic peptide storage and release
Kuroski de Bold, M L.
FASEB, Orlando, Florida, March 30-April 5, 2001.
Estrogen and the endocrine heart
Kuroski de Bold, M L.
XVII World Congress, International Society for Heart Research, Winnipeg, July 6 th -11 th , 2001
The use of animals in research: Twenty years of the discovery of ANF
Kuroski de Bold, M L.
Invited presentation to 4 th year Veterinary students at the University of Ottawa
University of Ottawa, June 2004
Pharmacological properties of CJC-1382, a long acting ANF analogue
Kuroski de Bold, M. L.
Guelph University, Veterinary College students
University of Ottawa, June 10, 2005
Natriuretic Peptide Analogues as Therapeutic Agents
Kuroski de Bold M. L.
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Molecular Basis of Pathological Cardiac Natriuretic Peptide Synthesis and Secretion
XXII Congreso Latinoamericano y 1ro Iberoamericano de Ciencias Fisiológicas
Buenos Aires, Argentina, November 4-7, 2006,
ANF and BNP as Therapeutic Agents
Kuroski de Bold M. L.
Cardiac Hormones as Biomarkers & Therapeutic Agents
16th World Congress of the World Society of Cardio-Thoracic Surgeons
Ottawa Congress Centre, Ottawa, Canada, August 17-20, 2006
Atrial Natriuretic Factor Analogues: Improving on Mother Nature
University of Ottawa Heart Institute
June 2006
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