Reproduction in Domestic Animals - Facultad de Ciencias Veterinarias

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16 t h International Congress on Animal Reproduction 94 Poster Abstracts 3rd and 6th month pregnant dromedaries were fixed in 4% (w/v) neutral formalin and embedded in paraffin wax. Morphometric examinations were carried out on Haematoxyline-Eosin stained sections. Histochemical reactions were performed to evaluate the secretion of proteins with the bromophenol blue method and carbohydrates by means of PAS (neutral mucosubstances / glycoproteins), Alcian blue (AB) pH 2.5 (carboxylated mucosubstances) and AB pH 1.0 (sulphated mucosubstances). In addition, the analysis of oligosaccharides was performed with 5 lectins in association with sialidase (s) treatment.Uterine glands from 6 month pregnant dromedary (6mp) were larger and the columnar epithelium was taller than 3 month pregnant (3mp) ones. Mean gland diameter was 60.0 ± 10.6 μm in 6mp and 47.8 ± 17.9 μm in 3mp (p
16 t h International Congress on Animal Reproduction Poster Abstracts 95 CP 42 rotational viscometer Brookfield model DV-I at a speed gradient of 0 s -1 to 230 s -1 . Shear stress (γ) was calculated from the measured viscosity profiles and the corresponding rheograms were plotted. Similar to what has been described in human semen, a pseudoplastic behavior which fit the following model: τ = K γ n (τ = shear stress; K= structural viscosity; γ= share rate; n= coefficient of consistency) was observed. Based on the rheogram, K and n were calculated. Descriptive analysis of the rheological characteristics was performed. Results The results obtained (Referential Values) were (mean +/- SD): (i) Apparent Viscosity at 11.5 s -1 = 46 +/- 15 cpoise, VC % 33%; (ii) Apparent Viscosity at 115 s -1 = 14 +/- 4 cpoise, VC % 29%; (iii) Structural Viscosity (K) = 2.3 +/- 0.5 dyne s / cm 2 , VC% 22%; (iv) Coefficient of Consistency (n) = 0.40 +/- 0.11. Conclusion Measuring viscosity using a cone plate rotational viscometer improves the repeatability and objectivity on semen viscosity evaluation. The rheological variables determined and the analysis of the rheological profile confirms the pseudoplastic behavior of llama semen. These results will offer a better understanding of the hiperviscosity in ejaculates of this specie, providing the basis for future studies on the effects of this characteristic on llama reproductive physiology. P202 Effect of different GnRH analogues and follicular size on ovulation and CL development in dromedary camels (Camelus Dromedarius) Nagy, P*, Juhasz, J Production & Veterinary Department, Emirates Industries for Camel Milk & Products, United Arab Emirates Buserelin is frequently used in dromedaries to induce ovulation, but Deslorelin, another synthetic GnRH analogue has not been tested yet (Tibary and Anouassi 1997). Recent data suggest that development of the corpus luteum is influenced by the size of the ovulatory follicle (Nagy et al 2005). The aim of these studies were (1) to compare the efficiency of two GnRH analogues, Buserelin and Deslorelin to induce ovulation; (2) to compare CL development after ovulation of small (< 1 cm) and large (1.5-2 cm) size dominant follicles. Two studies were carried out during the breeding season. In the first, 20 female dromedary camels were randomly selected into 2 treatment groups. Trans-rectal ultrasonography was performed daily. Animals were treated either with Buserelin (20 mikrog/animal, i.v.; Receptal, Intervet, Holland) or Deslorelin (2.1 mg/animal, sc. implant; Ovuplant, Peptid Technologies, Australia) when the dominant follicle reached 1.4-1.5 cm. Ovulation was detected with frequent ultrasonography. Blood samples for progesterone were collected on alternate days. In the second study, 4 dromedaries were given Buserelin twice: (a) when the dominant follicle exceeded 1.5 cm, (b) shortly after selection of the dominant follicle (< 1 cm). Blood samples were collected daily. Plasma progesterone in the first and second study was determined with RIA and ELISA, respectively. In the first study, all but one, Deslorelin treated camel ovulated. There was no significant difference in the mean time of ovulation between Buserelin (mean ± SEM; 28.9 ± 0.38 hours) and Deslorelin (30.9 ± 2.07 hours) treated animals. There was larger variation in the time of ovulation after Deslorelin (27 to 48 hours) than after Buserelin (27 to 30 hours). CL development and plasma progesterone concentration were similar after both treatments. In the second study, all camels ovulated large (mean ± SEM; 1.57 ± 0.05 cm) and small size (0.87 ± 0.06 cm, P
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Reproduction in Domestic Animals - Facultad de Ciencias Veterinarias

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