Reproduction in Domestic Animals - Facultad de Ciencias Veterinarias
Reproduction in Domestic Animals - Facultad de Ciencias Veterinarias
Reproduction in Domestic Animals - Facultad de Ciencias Veterinarias
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16 t h International Congress on Animal <strong>Reproduction</strong><br />
90 Poster Abstracts<br />
allele and genotype frequencies and Hardy-We<strong>in</strong>berg equilibrium<br />
were calculated. Allele frequency is 0.44 % for allele C and 0.56 %<br />
for allele T; population is <strong>in</strong> Hardy-We<strong>in</strong>berg equilibrium. Genotype<br />
frequency is 20% for the genotype C/C, 34.5% for T/T and 45.5% for<br />
C/T. Statistical analysis <strong>in</strong>dicated that buffaloes carry<strong>in</strong>g the genotype<br />
C/C showed their reproductive activity (7 vs. 4; P 0.05 and for G2, T1 = 15.0% (3/20) and<br />
T2 = 14.3% (3/21) P> 0.05. Although, when compar<strong>in</strong>g treatments of<br />
the two different groups T1 from G1, 57.1% vs T1 from G2, 15.0%;<br />
P< 0.05 and T2 from G1, 52.4% vs T2 from G2, 14.3%; P< 0.05 and<br />
T1 from G1, 57.1% vs T1 from G2, 15.0%, P< 0.05, it was observed<br />
statistical difference.<br />
Conclusions Although <strong>in</strong> the northeast of Brazil, the buffaloes are not<br />
submitted to a marked day length variation between the favorable and<br />
unfavorable reproductive seasons, the Ovsynch protocol did not show<br />
acceptable conception rate <strong>in</strong> the unfavorable season what may be due<br />
to the lack of progeserone dur<strong>in</strong>g this period. Yet, the use of half of<br />
the dose of GnRH <strong>in</strong> the second application showed to be as efficient<br />
as the full dose what reduces the cost of the protocol without<br />
compromis<strong>in</strong>g the conception rate.<br />
P189<br />
Production of sex-preselected embryos and offspr<strong>in</strong>g <strong>in</strong><br />
water buffalo (Bubalus bubalis)<br />
Lu, YQ 1 *; Liang, XW 2 ; Chen, MT 2 ; Zhang, XF 2 ; Lu, SS 1 ; Zhang, M 1 ; Pang,<br />
CY 2 ; Lu, KH 1<br />
1Animal <strong>Reproduction</strong> Institute, Guangxi Key Laboratory of Subtropical<br />
Bioresource Conservation and Utilization, Guangxi University, Nann<strong>in</strong>g<br />
530004, Ch<strong>in</strong>a; 2 Guangxi Buffalo Research Institute, Ch<strong>in</strong>ese Aca<strong>de</strong>my of<br />
Agricultural Science, Nann<strong>in</strong>g 530001, Ch<strong>in</strong>a<br />
Buffalo is an important livestock resource <strong>in</strong> many Asian and<br />
Mediterranean countries. Sex-preselection via sperm sex<strong>in</strong>g would be<br />
of great <strong>in</strong>terest <strong>in</strong> buffalo species both <strong>in</strong> biological and economic<br />
terms. The objective of the present study was to evaluate the potential<br />
of OPU-IVEP system to produce sexed embryos and offspr<strong>in</strong>g <strong>in</strong><br />
buffalo species. Buffalo sperm was sorted <strong>in</strong>to X-sperm enriched<br />
population follow<strong>in</strong>g the general procedure <strong>de</strong>scribed by Johnson and<br />
Welch (Theriogenology 1999;52:1323-41). Oocytes were picked up<br />
from fertile Murrah and Nili-Ravi buffalo cows by ultrasound<br />
transvag<strong>in</strong>al ovum retrieval. The oocytes (gra<strong>de</strong> A and B) <strong>de</strong>rived<br />
from OPU were matured <strong>in</strong> TCM199 supplemented with 5% estrous<br />
cow serum and 10 μg/mL FSH and then fertilized <strong>in</strong> vitro with sexed<br />
or unsexed sperm <strong>in</strong> modified Tyro<strong>de</strong>’s medium supplemented with<br />
0.6% BSA, 2.0 mM caffe<strong>in</strong>e and 20 µg/mL Hepar<strong>in</strong> (Anim Reprod<br />
Sci, 2007;100:192-6). Twenty cycl<strong>in</strong>g Murrah and Nili-Ravi buffaloes<br />
were repeatedly submitted to OPU <strong>in</strong> this study. An average of 4.6<br />
oocytes was retrieved per session per animal, 70.9% of which was<br />
Gra<strong>de</strong> A and B. A total of 1064 oocytes were submitted to <strong>in</strong> vitro<br />
maturation and fertilization. The results <strong>in</strong>dicated that the<br />
<strong>de</strong>velopmental competence of oocytes fertilized with sexed and