Reproduction in Domestic Animals - Facultad de Ciencias Veterinarias
Reproduction in Domestic Animals - Facultad de Ciencias Veterinarias
Reproduction in Domestic Animals - Facultad de Ciencias Veterinarias
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16 t h International Congress on Animal <strong>Reproduction</strong><br />
Poster Abstracts 85<br />
Grants: CICYT-FEDER AGL 2005-02614, CICYT-FEDER AGL<br />
2007-61229 and DGA A-26/2005.<br />
P172<br />
Plasmatic progesterone and cortisol concentrations <strong>in</strong><br />
non-pregnant, pregnant and lactat<strong>in</strong>g Saanen breed goats<br />
De Paula, M 1 ; Peruca Bald<strong>in</strong>i, L 1 *; Greco, G 1 ; Bittencourt, RF 1 ; Maia, L 2 ; Oba, E 1<br />
1Department of Radiology and Animal <strong>Reproduction</strong>, São Paulo State<br />
University -UNESP- Botucatu, Brazil; 2 Department of Veter<strong>in</strong>ary Cl<strong>in</strong>ics and<br />
Pathology, Flum<strong>in</strong>ense Fe<strong>de</strong>ral University -UFF, Brazil<br />
Progesterone is well known as be<strong>in</strong>g the ma<strong>in</strong> hormone capable of<br />
ma<strong>in</strong>ta<strong>in</strong><strong>in</strong>g pregnancy <strong>in</strong> domestic animals. Cortisol, on the other<br />
hand, is released dur<strong>in</strong>g stress, be<strong>in</strong>g responsible for the <strong>in</strong>duction of<br />
parturition through stimulation of progesterone catalysis. In the<br />
bov<strong>in</strong>e species, high cortisol concentrations have been <strong>de</strong>tected<br />
moments before birth and dur<strong>in</strong>g the puerperal period. In comparison<br />
to other domestic rum<strong>in</strong>ants, few studies have been published<br />
regard<strong>in</strong>g the variations <strong>in</strong> the plasmatic concentrations of cortisol <strong>in</strong><br />
goats, accord<strong>in</strong>g to their reproductive state. The present work had as<br />
objective to <strong>de</strong>term<strong>in</strong>e the plasmatic concentrations of progesterone<br />
and cortisol <strong>in</strong> non-pregnant, pregnant and lactat<strong>in</strong>g Saanen breed<br />
goats. Forty-three (43) female Saanen goats, ag<strong>in</strong>g 24 to 32 months,<br />
were assigned <strong>in</strong>to three different groups accord<strong>in</strong>g to their<br />
reproductive state. Group 1 was composed of 15 non-pregnant goats.<br />
Thirteen (13) goats experienc<strong>in</strong>g their last month of pregnancy were<br />
assigned to group 2. As for group 3, it was composed of 15 lactat<strong>in</strong>g<br />
goats, whose kids were at most one month old. Blood was collected<br />
between 8:00 and 10:00 am through jugular venipuncture <strong>in</strong>to tubes<br />
conta<strong>in</strong><strong>in</strong>g hepar<strong>in</strong>, which were centrifugated at 3000 x g for 15<br />
m<strong>in</strong>utes. Plasma obta<strong>in</strong>ed was immediately stored at – 20 <strong>de</strong>grees<br />
Celsius. Progesterone and cortisol concentrations were estimated<br />
through radioimmunoassay, us<strong>in</strong>g Diagnostic Products Corporation®<br />
(DPC) kits. The obta<strong>in</strong>ed data was statistically analyzed through the<br />
Statistical Analysis System®, version 6.1, 1996. The concentrations<br />
of progesterone and cortisol were compared between the groups us<strong>in</strong>g<br />
the Stu<strong>de</strong>nt-Newman-Keuls test. The correlation between both<br />
hormones measured concentrations was established us<strong>in</strong>g the PROC<br />
CORR procedure. Significance levels were set as P < 0.05. No<br />
correlation was found between cortisol and progesterone<br />
concentrations (P > 0.05). Mean progesterone concentrations were<br />
1.71 ng/mL +/- 2.18, 13.56 ng/mL +/- 5.21 and 0.2 ng/mL +/- 0.08 <strong>in</strong><br />
groups 1, 2 and 3, respectively. Pregnant goats had higher (P < 0.01)<br />
progesterone concentrations than lactat<strong>in</strong>g and non-pregnant animals.<br />
As for cortisol, mean concentrations were, respectively, 1.75 μg/dL<br />
+/- 1.04, 1.21 μg/dL +/- 0.4 and 1.17 μg/dL +/- 0.6 <strong>in</strong> groups 1, 2 and<br />
3. Plasmatic cortisol concentrations were similar between the<br />
analyzed groups. Progesterone concentrations were higher <strong>in</strong> pregnant<br />
than <strong>in</strong> lactat<strong>in</strong>g and non-pregnant animals. No correlation between<br />
both hormones was found.<br />
P173<br />
Reproductive parameters of dairy goats submitted to<br />
artificial bioclimatic conditions similar to the Eastern<br />
Amazon Region<br />
P<strong>in</strong>ho, RO. 1 ; Guimarães, JD. 1 *; Mart<strong>in</strong>s, LF. 1 ; Castilho, EF. 1 ; Borges, MCB. 1 ;<br />
Paraizo, RM. 1 ; Torres, CAA 2 ; Vasconcelos, GSC 1 .<br />
Veter<strong>in</strong>ary Medic<strong>in</strong>e Department, Viçosa Fe<strong>de</strong>ral University, Brazil;<br />
2Zootechnics Department, Viçosa Fe<strong>de</strong>ral University, Brazil<br />
This work <strong>de</strong>als with the reproductive behavior of Alp<strong>in</strong>e and Saanen<br />
female goats submitted to artificial bioclimatic conditions similar to<br />
those of the Eastern Amazon Region, when compared to female goats<br />
raised un<strong>de</strong>r normal typical bioclimatic conditions of regions where<br />
they <strong>de</strong>monstrate seasonality. The study was conducted dur<strong>in</strong>g the<br />
reproductive season for goats, consist<strong>in</strong>g of an adaptation period of 30<br />
days and an experimental period of 60 days, <strong>in</strong> the bioclimatic<br />
chamber. Group 1 (n=4) animals rema<strong>in</strong>ed <strong>in</strong> the bioclimatic chamber<br />
with temperature and air humidity control (8:00-12:00 hours: 30 ºC;<br />
12:00-18:00: 36 ºC; 18:00-8:00: 26 ºC; with 60 % of average<br />
humidity; and a 12 hour fotoperiod), thus simulat<strong>in</strong>g bioclimatic<br />
conditions of the northern region of Brazil (next to the Equator l<strong>in</strong>e),<br />
whereas group 2 (n=4) was kept un<strong>de</strong>r <strong>in</strong>fluence of the natural<br />
climatic variations of the season. Physiological parameters were<br />
measured twice a day, with daily follicular dynamics accompaniment,<br />
besi<strong>de</strong>s blood collection twice a week for cortisol, progesterone and<br />
estrogen dosages. Dur<strong>in</strong>g the experimental period, a difference was<br />
observed (p0.05). There was no difference (p>0.05) <strong>in</strong> the duration of estral<br />
cycle and estrus for the animals of groups 1 and 2. There was no<br />
difference (p>0.05) <strong>in</strong> relation to the number of follicles observed <strong>in</strong><br />
the day of the estrus and to the ovulatory follicle diameter, as a<br />
function of the groups and number of estrus evaluated, with average<br />
values of 4 and 3.5 <strong>in</strong> the 1 st estrus, 5 and 3 <strong>in</strong> the 2 nd estrus, and 4 and<br />
4.5 <strong>in</strong> the 3 rd estrus, for groups 1 and 2, respectively. The number of<br />
follicular waves observed varied from 4 to 5 <strong>in</strong> group 1 and 2 to 4<br />
waves <strong>in</strong> group 2. Although the animals of group 1 <strong>de</strong>monstrated<br />
higher values of progesterone and estrogen <strong>in</strong> relation to the animals<br />
of group 2, the endocr<strong>in</strong>e secretion standard of these hormones<br />
revealed to be similar for both groups <strong>in</strong> all the studied estral cycles,<br />
as a function of time. The results <strong>in</strong>dicated that female goats can be<br />
raised un<strong>de</strong>r bioclimatic conditions, without modify<strong>in</strong>g the related<br />
physiological standards.<br />
P174<br />
Relation between superovulatory response and embryo<br />
quality with hematological and biochemical blood<br />
variables <strong>in</strong> hair ewes<br />
Ramón, J 1 *, Sauri, I 2 , Navarrete, L 1 , González, E 2 , Cervera, D 1 , Sierra, A 1 ,<br />
Piña, R 3 and Qu<strong>in</strong>tal, J 4<br />
1Center of Ov<strong>in</strong>e Selection and <strong>Reproduction</strong>, Technical Institute of Conkal,<br />
Yuc., Mexico (J. Ramón jramon@itaconkal.edu.mx); 2 Central Regional<br />
Laboratory of Merida, Yuc, Mexico. 3 Faculty of Medic<strong>in</strong>e, Autonomous<br />
University of Yucatan, 4 INIFAP-Mococha, Yucatán, México<br />
Our aim was to correlate hematological (hematocrit and hemoglob<strong>in</strong>)<br />
and biochemical blood variables (plasmatic prote<strong>in</strong>s, total prote<strong>in</strong>s,<br />
album<strong>in</strong>, glucose, cholesterol, creat<strong>in</strong><strong>in</strong>e, urea, ureic acid, alan<strong>in</strong>e<br />
am<strong>in</strong>otransferase, aspartate am<strong>in</strong>otransferase and alkal<strong>in</strong>e<br />
phosphatase) with ovulation rate and embryo quality <strong>in</strong> Pelibuey<br />
sheep. No significant relationships were found (P > 0.05) between<br />
ovulation rate and such variables. However, significant difference was<br />
found (P < 0.05) between ureic acid and embryo quality a correlation<br />
of 0.4686. By us<strong>in</strong>g a regression analysis, this equation was obta<strong>in</strong>ed:<br />
embryo quality = 3.4050 + 1.5549*Ureic acid. This equation<br />
expresses that for every 0.3 mg/dL <strong>in</strong>crement <strong>in</strong> ureic acid levels, the<br />
embryo quality improves 1.55 units of score. The levels of ureic acid<br />
were proportionally <strong>in</strong>verse to urea, show<strong>in</strong>g a ten<strong>de</strong>ncy (P < 0.1)<br />
only with creat<strong>in</strong><strong>in</strong>e over embryo quality. The supplementation before<br />
and after the superovulatory treatment showed differences, on<br />
hematocrit (P < 0.01), hemoglob<strong>in</strong> (P < 0.01), glucose (P < 0.05), urea<br />
(P < 0.05) and alkal<strong>in</strong>e phosphatase (P < 0.01). These results suggest<br />
that biochemical parameters such as ureic acid, urea and creat<strong>in</strong><strong>in</strong>e,<br />
may be <strong>in</strong>volved together with methabolic pathways that contribute to<br />
predict or expla<strong>in</strong> levels of superovulatory response and embryo<br />
quality with the applicattion of exogenous gonadothrop<strong>in</strong>s.<br />
Supported by: DGEST 509.07-P and CONACYT-SAGARPA-2004-<br />
C01-150/A-1