Reproduction in Domestic Animals - Facultad de Ciencias Veterinarias

16 t h International Congress on Animal Reproduction
82 Poster Abstracts
P163
Ram sperm morphometry: intra-individual variation and
relation with fertility
Mata-Campuzano, M. 2 *; Garcia-Macias, V. 1 ; Anel, L. 1 , Alvarez, M. 1 ; Bernardo,
J. 1 ; Chamorro, C 3 ; De Paz, P. 2
1Animal Reproduction and Obstetrics, University of Leon, 24071, León, Spain;
2Cell Biology, University of Leon, 24071, León, Spain; 3 Veterinary Anatomy;
University of Leon, 24071, León, Spain
Sperm head morphometry provides an objective analysis of a semen
sample since the development of computer assisted analysis
technology. Relationship between fertility and normal shape of
spermatozoa morphology has been studied widely in both humans and
animals.
This work has to main objectives: 1. to determine if sperm head
morphometry and fertility are related in ram sperm (assessing
ejaculates from 24 Assaf rams), 2. to test if there were differences
among the ejaculates of the same animal (6 animals, 3 separated
ejaculates, two weeks interval). All ejaculates were collected by
artificial vagina and diluted in extender (tris 3.322 g, citric acid 1.737
g, fructose 0.954 g and distilled water 100 ml) to a final concentration
of 400×10 6 spermatozoa/ml. Samples were fixed in 2% glutaraldehide
in BL1 medium (glucose 2.9 g, sodium citrate dehydrated 1.0 g
sodium bicarbonate 0.2 g and distilled water 100mL). Microscope
slides were prepared by placing a 5µl drop of the extended semen at
the edge of a frosted slide and dragging the drop across it. Slides were
air dried for at least two hours and stained using a Diff-Quik staining
method (QCA, Tarragona, Spain). Subsequently, slides were rinsed in
distilled water and air dried, then examined with a bright field
microscope at a magnification of 600x. At least 100 properly digitized
sperm heads for each sample were analyzed with a computer assisted
sperm morphology assessment (CASMA, integrated semen analysis
system v 1.0.9 PROISER, Valencia, Spain) system. Each sperm head
was measured for length (L, µm), width (W, µm), area (A, µm 2 ),
perimeter (P, µm); and four derived parameters of head shape:
elipticity (ELI): L/W; rugosity (RU): 4πA/P 2 ; elongation (ELO): (L-
W)/(L+W); and regularity (RE): π LW/4A. Mean values were L: 8.67;
W: 4.92; A: 36.37; P: 24.12; ELI: 1.76; RU: 0.78; ELO: 0.27; RE:
0.94.
In experiment 1 our results show statistically significant differences
(p0.05) with
fertility. Sample preparation and analysis techniques should be
improved in order to obtain more accurate results.
This work was supported in part by CICYT (AGL2005-07601),
Diputación de León and Junta de Castilla y León.
P164
Effects of aflatoxin B1 on ram epididymal and ejaculatory
sperm motility
Mirshokraei, P 1 *, Tajik, P 2 , Khosravi, A 3
1Department of Clinical Sciences, Faculty of Veterinary Medicine, Shahrekord
University, Shahrekord, Iran; 2 Department of Clinical Sciences, Faculty of
Veterinary Medicine, University of Tehran, Iran; 3 Department of Microbiology,
Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran
In order to study the effects of aflatoxin on ovine spermatozoa,
epididymal and ejaculatory sperm were added different concentrations
of aflatoxin B1. When ram epididymal sperm were exposed to
different concentrations of aflatoxin, one-hour post incubation in
control group, 68.16% were motile, significantly (P