Reproduction in Domestic Animals - Facultad de Ciencias Veterinarias

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16 t h International Congress on Animal Reproduction 76 Poster Abstracts Methods Twenty-eight goats that had tested positive for CAEV using PCR on vaginal secretions were used as embryo donors. Embryos with intact ZP were selected and washed ten times; they were then frozen and used for transfer into CAEV-free recipient goats. Nineteen of the forty-nine recipient goats gave birth, producing a total of 23 kids. Three blood samples were taken from each recipient goat, ten days before, during, and ten days after parturition; these were tested for CAEV antibodies using ELISA and for CAEV proviral DNA using PCR. The mothers were then euthanized. Tissue samples were taken from the lungs, udder, and retromammary and prescapular lymph nodes. The kids were separated from their mothers at birth. Seven of them died. At 4 months of age, 16 kids were subjected to drug-induced immunosuppression. Blood samples were taken every month from birth to 4 months of age; samples were then taken on day 15, day 21, and day 28 after the start of the immunosuppressive treatment. The kids were then euthanized and tissue samples taken from the carpal synovial membrane, lung tissue, prescapular lymph nodes, inguinal and retro-mammary lymph nodes, and uterus. Results All samples from the 19 recipient goats and 23 kids were found to be negative for CAEV antibodies and/or CAEV proviral DNA. Conclusion This study, performed under field conditions, clearly demonstrates that embryo transfer can be used to produce CAEV-free kids from CAEV-infected biological mothers. Indeed, none of the 16 kids collected from infected mothers at the embryonic stage, transferred to CAEV-free recipient goats, and subjected to immunosuppressive treatment at 4 months of age, were found to be positive for CAEV with any of the diagnostic methods used in all of the analyzed target tissues of the virus. Similarly, none of the 20 recipient goats seroconverted, and none of the sampled tissues tested positive for CAEV proviral DNA. P145 Effects of a mutation in Bone Morphogenetic Protein 15 gene (BMP15) on natural ovulation rate and on the response to superovulatory FSH treatment in Rasa Aragonesa ewes Folch, J 1 *; Alabart, JL 1 ; Martínez-Royo, A 1 ; Echegoyen, E 1 ; Cocero, MJ 2 ; Jurado, JJ 3 ; Bodin, L 4 ; Calvo, JH 1 1Unidad de Tecnología en Producción Animal. CITA. Av. de Montañana, 930. 50059-Zaragoza, Spain; 2 Dpto. Reproducción Animal. INIA. Av. Puerta de Hierro s/n. 28040-Madrid, Spain; 3 Dpto. Mejora Genética animal. INIA. Ctra. La Coruña Km 7.5. 28040-Madrid, Spain; 4 INRA, UR631 Station d'amélioration génétique des animaux, F-31326 Castanet-Tolosan, France A MOET Program is applied to improve the efficiency of a selection scheme for prolificacy in Rasa Aragonesa ovine breed. We have recently found a new mutation in BMP15 gene (1) in the highest prolific ewes of the Scheme. Since similar mutations are associated to higher ovulation rate (OR) in heterozygous females in other ovine breeds, we carried out a study to confirm the effect of this new mutation on OR in Rasa Aragonesa, in both natural conditions (Experiment 1) or after superovulation with oFSH (Experiment 2). Experiment 1 Two lots of ewe lambs of similar age, body weigh and body condition score were compared: BMP15 group (mutant animals) and control group (non-mutant, non-selected). All animals were maintained indoor and feeded ad libitum. At 9 months of age, lambs received FGA sponges. The OR was recorded six days after sponge withdrawal by laparoscopy and was repeated 17 and 34 days later. No males were used for heats detection. OR in Control group was similar to previously reported values in nonselected Rasa Aragonesa ewe lambs, while BMP15 group presented about 0.6 extra ovulations. Genotype n OR1 OR2 OR3 OR (Pooled) BMP15 15 1.85 a 1.69 a 1.58 c 1.71 a Control 18 1.09 b 1.14 b 1.13 d 1.12 b n: Number of ewe lambs; OR: Ovulation rate in lambs ovulating a, b: P
16 t h International Congress on Animal Reproduction Poster Abstracts 77 P147 The Effect of an Opioid Antagonist on Blood Progesterone levels of crossbreed ewes with GnRH induced short luteal phase during the anoestrus season Fuentes, HV-O. 1 *, Fuentes, CPI. 2 , Moreno, H. 3 , Parra, VM. 1Centro Universitario de los Altos, Universidad de Guadalajara, Km 7.5 Carretera a Yahualica, Tepatitlan de Morelos Jalisco CP47600 (Víctor Octavio Fuentes Hernández Ph.D. victoro@servidor.unam.mx; vfuentes@cualtos.udg.mx); 2 Department of Anesthesiology, Hospital Pemex Sur Alta Especialidad DF , Mexico; 3 Facultad de Medicina Veterinaria y Zootecnia, Universidad Michoacana de San Nicolas Hgo, Mexico With the objective of studying the effect of an opioid antagonist on progesterone levels in ewes with induced short luteal phase during the anoestrous season. A group of 20 crossbreed ewes was used. Age flutuated between 2 and 6 years. Housed in oppen paddocks with food and choped barly hay ad libitum and complemented with 250 g of concentrated feed. 10 ewes selected at random were implanted with 15 mg naloxone using cristaline microcelulose as a vehicle. The remainig 10 ewes were used as control and were implanted with a capsule of cristaline microcelulose free of any medicament. Seven days after implant both groups; treated and control; were injected with GnRH (250 ng iv at two hour intervals during 24 hours) at the end of treatment 125 µg of GnRH was injected iv. Blood samples were collected at 12 th hrs intervals since the comencement of the experiment and sampling continued for 15 days after the last injection of GnRH. It was observed that in two of the control ewes progesterone levels increased 36 hrs after GnRH treatment, reaching a highest level on day 7, on the 8 remaining control ewes short luteal phases were observed with maximal progesterone concentrations of 1 ng/ml. 3 ewes treated with naloxone showed short luteal phase, while in the remaining 7 ewes treated with naloxone, progesterone levels were similar to those observed in control ewes with normal luteal phase. During the statistical analysis a significant effect fue to treatment was detected (P
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Reproduction in Domestic Animals - Facultad de Ciencias Veterinarias

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