Reproduction in Domestic Animals - Facultad de Ciencias Veterinarias
Reproduction in Domestic Animals - Facultad de Ciencias Veterinarias
Reproduction in Domestic Animals - Facultad de Ciencias Veterinarias
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16 t h International Congress on Animal <strong>Reproduction</strong><br />
76 Poster Abstracts<br />
Methods Twenty-eight goats that had tested positive for CAEV us<strong>in</strong>g<br />
PCR on vag<strong>in</strong>al secretions were used as embryo donors. Embryos<br />
with <strong>in</strong>tact ZP were selected and washed ten times; they were then<br />
frozen and used for transfer <strong>in</strong>to CAEV-free recipient goats. N<strong>in</strong>eteen<br />
of the forty-n<strong>in</strong>e recipient goats gave birth, produc<strong>in</strong>g a total of 23<br />
kids. Three blood samples were taken from each recipient goat, ten<br />
days before, dur<strong>in</strong>g, and ten days after parturition; these were tested<br />
for CAEV antibodies us<strong>in</strong>g ELISA and for CAEV proviral DNA<br />
us<strong>in</strong>g PCR. The mothers were then euthanized. Tissue samples were<br />
taken from the lungs, ud<strong>de</strong>r, and retromammary and prescapular<br />
lymph no<strong>de</strong>s. The kids were separated from their mothers at birth.<br />
Seven of them died. At 4 months of age, 16 kids were subjected to<br />
drug-<strong>in</strong>duced immunosuppression. Blood samples were taken every<br />
month from birth to 4 months of age; samples were then taken on day<br />
15, day 21, and day 28 after the start of the immunosuppressive<br />
treatment. The kids were then euthanized and tissue samples taken<br />
from the carpal synovial membrane, lung tissue, prescapular lymph<br />
no<strong>de</strong>s, <strong>in</strong>gu<strong>in</strong>al and retro-mammary lymph no<strong>de</strong>s, and uterus.<br />
Results All samples from the 19 recipient goats and 23 kids were<br />
found to be negative for CAEV antibodies and/or CAEV proviral<br />
DNA.<br />
Conclusion This study, performed un<strong>de</strong>r field conditions, clearly<br />
<strong>de</strong>monstrates that embryo transfer can be used to produce CAEV-free<br />
kids from CAEV-<strong>in</strong>fected biological mothers. In<strong>de</strong>ed, none of the 16<br />
kids collected from <strong>in</strong>fected mothers at the embryonic stage,<br />
transferred to CAEV-free recipient goats, and subjected to<br />
immunosuppressive treatment at 4 months of age, were found to be<br />
positive for CAEV with any of the diagnostic methods used <strong>in</strong> all of<br />
the analyzed target tissues of the virus. Similarly, none of the 20<br />
recipient goats seroconverted, and none of the sampled tissues tested<br />
positive for CAEV proviral DNA.<br />
P145<br />
Effects of a mutation <strong>in</strong> Bone Morphogenetic Prote<strong>in</strong> 15<br />
gene (BMP15) on natural ovulation rate and on the<br />
response to superovulatory FSH treatment <strong>in</strong> Rasa<br />
Aragonesa ewes<br />
Folch, J 1 *; Alabart, JL 1 ; Martínez-Royo, A 1 ; Echegoyen, E 1 ; Cocero, MJ 2 ;<br />
Jurado, JJ 3 ; Bod<strong>in</strong>, L 4 ; Calvo, JH 1<br />
1Unidad <strong>de</strong> Tecnología en Producción Animal. CITA. Av. <strong>de</strong> Montañana, 930.<br />
50059-Zaragoza, Spa<strong>in</strong>; 2 Dpto. Reproducción Animal. INIA. Av. Puerta <strong>de</strong><br />
Hierro s/n. 28040-Madrid, Spa<strong>in</strong>; 3 Dpto. Mejora Genética animal. INIA. Ctra.<br />
La Coruña Km 7.5. 28040-Madrid, Spa<strong>in</strong>; 4 INRA, UR631 Station<br />
d'amélioration génétique <strong>de</strong>s animaux, F-31326 Castanet-Tolosan, France<br />
A MOET Program is applied to improve the efficiency of a selection<br />
scheme for prolificacy <strong>in</strong> Rasa Aragonesa ov<strong>in</strong>e breed. We have<br />
recently found a new mutation <strong>in</strong> BMP15 gene (1) <strong>in</strong> the highest<br />
prolific ewes of the Scheme. S<strong>in</strong>ce similar mutations are associated to<br />
higher ovulation rate (OR) <strong>in</strong> heterozygous females <strong>in</strong> other ov<strong>in</strong>e<br />
breeds, we carried out a study to confirm the effect of this new<br />
mutation on OR <strong>in</strong> Rasa Aragonesa, <strong>in</strong> both natural conditions<br />
(Experiment 1) or after superovulation with oFSH (Experiment 2).<br />
Experiment 1<br />
Two lots of ewe lambs of similar age, body weigh and body condition<br />
score were compared: BMP15 group (mutant animals) and control<br />
group (non-mutant, non-selected). All animals were ma<strong>in</strong>ta<strong>in</strong>ed<br />
<strong>in</strong>door and fee<strong>de</strong>d ad libitum. At 9 months of age, lambs received<br />
FGA sponges. The OR was recor<strong>de</strong>d six days after sponge withdrawal<br />
by laparoscopy and was repeated 17 and 34 days later. No males were<br />
used for heats <strong>de</strong>tection.<br />
OR <strong>in</strong> Control group was similar to previously reported values <strong>in</strong> nonselected<br />
Rasa Aragonesa ewe lambs, while BMP15 group presented<br />
about 0.6 extra ovulations.<br />
Genotype n OR1 OR2 OR3<br />
OR<br />
(Pooled)<br />
BMP15 15 1.85 a 1.69 a 1.58 c 1.71 a<br />
Control 18 1.09 b 1.14 b 1.13 d 1.12 b<br />
n: Number of ewe lambs; OR: Ovulation rate <strong>in</strong> lambs ovulat<strong>in</strong>g<br />
a, b: P