Reproduction in Domestic Animals - Facultad de Ciencias Veterinarias
Reproduction in Domestic Animals - Facultad de Ciencias Veterinarias
Reproduction in Domestic Animals - Facultad de Ciencias Veterinarias
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16 t h International Congress on Animal <strong>Reproduction</strong><br />
Poster Abstracts 75<br />
<strong>de</strong>term<strong>in</strong>ed accord<strong>in</strong>g to lamb<strong>in</strong>g dates. In Exp.2 the whole protocol<br />
was repeated with 115 spr<strong>in</strong>g lamb<strong>in</strong>g dams (Exp.2 d0:22 th June). In<br />
Exp.1 39% of ewes were still cycl<strong>in</strong>g <strong>in</strong> Febr, however only 6%<br />
rema<strong>in</strong>ed cyclic by the end of April (NS). Significantly higher percent<br />
of gestagen treated groups ovulated follow<strong>in</strong>g synchronisation<br />
(Gest:96% vs Mel+Gest:95% vs Mel+GPG:45%;P=0.040). 14% of<br />
Gest and Mel+Gest group vs 3% of Mel+GPG concieved from Fix AI<br />
(NS). 10% Gest, 5% Mel+Gest, 3% Mel+GPG became pregnant after<br />
ram <strong>in</strong>troduction (NS). Great proportion 31-43% conceived <strong>in</strong> the<br />
follow<strong>in</strong>g breed<strong>in</strong>g season. 38-62% rema<strong>in</strong>ed unpregnant for more<br />
than 220 days. In Exp.2 4% of dams were cycl<strong>in</strong>g by d0 (June, NS).<br />
Proportion of cyclic animals <strong>in</strong> Mel treated groups ten<strong>de</strong>d to be higher<br />
by d45-d56 (19% Gest vs 44% Mel+Gest vs 47%<br />
Mel+GPG;P=0.109). 100% of Gest and Mel+Gest vs 88% of<br />
Mel+GPG ewes ovulated follow<strong>in</strong>g synchronisation (NS), however<br />
only 24% (Gest), 22% (Mel+Gest), 8% (Mel+GPG) conceived from<br />
fix AI (P=0.190). In all treat<strong>in</strong>g groups 65% became pregnant after<br />
ram <strong>in</strong>troduction (NS). 8-27% of dams rema<strong>in</strong>ed unpregnant until<br />
d150 (NS). Survival analysis of day of conception showed no<br />
significant difference between treatment groups (Exp.1<br />
P=0.361;Exp.2 P=0.131). Concud<strong>in</strong>g our f<strong>in</strong>d<strong>in</strong>gs reproductive<br />
activity of Awassi sheep became markedly seasonal un<strong>de</strong>r temperate<br />
latitu<strong>de</strong>. Slow release melaton<strong>in</strong> implant <strong>in</strong>serted <strong>in</strong> Febr could not<br />
<strong>in</strong>duce cyclic ovarian function; however the same treatment had<br />
beneficial effect when used <strong>in</strong> June. GPG protocol as a possible<br />
alternative of longterm gestagen treatment for synhronisation for AI<br />
can only be effective when used near to the natural breed<strong>in</strong>g season.<br />
P142<br />
Effects of different egg yolk concentration and sodium<br />
do<strong>de</strong>cyl sulfate dur<strong>in</strong>g the freez<strong>in</strong>g step of<br />
cryopreservation on motility and viability of Markhoz goat<br />
spermatozoa<br />
Farshad, A.*, Khalili, B. and Fazeli, P.<br />
Laboratory of Animal <strong>Reproduction</strong> of Department of Animal Science, College<br />
of Agriculture, University of Kur<strong>de</strong>stan, Sannandaj, Iran (Farshad, A.<br />
AFarshad@uok.ac.ir)<br />
Cryopreservation <strong>in</strong>duces cold shock and partially irreversible damage<br />
to sperm membranes. Egg yolk and addition of sodium do<strong>de</strong>cyl sulfat<br />
(SDS) to exten<strong>de</strong>rs prevents the effects of cold shock. Therefore, the<br />
objective of our study was to evaluate the effects of egg yolk and SDS<br />
on the cryopreservation of spermatozoa of goat.<br />
In Experiment 1, effects of different concentrations of egg yolk were<br />
<strong>in</strong>vestigated. Semen diluted (1: 4) with Tris-citric acid-fructoseglycerol<br />
solution conta<strong>in</strong><strong>in</strong>g 5, 10, 15 and 20 % (v/v) egg yolk were<br />
packed <strong>in</strong> 0.25 ml French straws at 37°C and cooled to 5°C over 2 h.<br />
Than, samples were frozen <strong>in</strong> vapor of liquid nitrogen, stored <strong>in</strong> liquid<br />
nitrogen for 24 h and thawed at 37°C for 1 m<strong>in</strong>. After thaw<strong>in</strong>g,<br />
samples were evaluated for motility (%), progressive motility (%),<br />
viability (%) and morphological abnormality (%).<br />
The results shows, the rates of motility, progressive motility, viability<br />
and morphological abnormality <strong>in</strong> the dilution conta<strong>in</strong><strong>in</strong>g 5% egg yolk<br />
(55.7±4, 42.3±6, 60.2±4 and 89.5±3, respectively) were significantly<br />
higher (P0.05) between the 10 and 15 % egg yolk groups. The<br />
parameters of spermatozoa diluted <strong>in</strong> exten<strong>de</strong>r conta<strong>in</strong><strong>in</strong>g of 20 % egg<br />
yolk were significantly <strong>de</strong>creased (P