Reproduction in Domestic Animals - Facultad de Ciencias Veterinarias

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16 t h International Congress on Animal Reproduction 70 Poster Abstracts P126 Accuracy of ultrasonography in the diagnosis of silent heat in cows compared to plasma progesterone concentration Zdunczyk, S*; Janowski, T; Ras, M; Baranski, W Department of Animal Reproduction, University of Warmia and Mazury in Olsztyn, Poland Silent heat is defined as the lack of behavioral oestrus symptoms although the genital organs are undergoing the normal cyclical changes. It is the main reason of post-partum anoestrus in dairy cows causing elongation of service period and, in consequence, substantial economical losses. Rectal palpation is a main method used for clinical evaluation of ovarian activity in dairy herds, but it may cause high proportion of misdiagnosed and incorrectly treated animals. Ultrasonography is considered an important diagnostic aid to rectal palpation. The aim of this study was to assess the accuracy of ultrasonography for the diagnosis of silent heat compared to plasma progesterone concentration. The study was carried out in 5 dairy herds in North-East Poland. Cows, which showed no visible oestrus signs until day 60 postpartum were examined by ultrasonography twice, in a 10-day interval. A real-time, B-mode scanner (Honda 1500) with 5 MHz probe was used. Blood samples were collected simultaneously from the tail vein into heparinised evacuated tubes. Progesterone concentration in blood plasma was determined using RIA. Presence of physiological ovarian structures (follicles, corpus luteum) was an indication of cyclicity in anoestrous cows. High progesterone level on the first, but low on the second examination or low on the first and high on the second examination were interpreted as a silent heat. Based on progesterone values silent heat was diagnosed in 145 anoestrous cows, whereas ultrasonographically 106 cows were found with silent heat. The accuracy of ultrasonography in diagnosis of silent heat in cows was 89.0 %. The sensitivity and specificity of this method for diagnosis of corpus luteum were 94.7 % and 84.0 %, respectively. Our results showed that ultrasonography is useful tool to diagnose of silent heat in cows. Poster 02 - Reproduction of Small Ruminants P127 Trehalose improves ram semen cryopreservation when it is present in conventional extenders Aisen, E.*, Pelufo, V., Malcotti, V., Morello, H., Medina, V., Venturino, A. Laboratorio de Teriogenología, Facultad de Ciencias Agrarias, Universidad Nacional del Comahue. C.C. 85 (8303), Cinco Saltos (Río Negro), Argentina It is well known that trehalose improve the post-thawing sperm viability and fertility when it is added in hypertonic conditions. The aim of this study was to apply trehalose in three different extenders used commonly in ram semen cryopreservation. The solutions (with or without trehalose 100 mosm/L), containing Tris, citric acid, fructose or glucose, glycerol, egg yolk and antibiotics, were B1 (experimental), S (Salamon’s extender) and Tri (Triladyl®). Ejaculates from four Merino rams were evaluated and pooled at 30°C. The semen was diluted to contain 1x10 9 cells/mL, cooled to 5°C, loaded into 0.25-mL straws, frozen and stored in liquid nitrogen. Postthaw evaluation was based on sperm motility (MI), supravital stain (Eo), acrosome integrity (AI), hyposmotic swelling test (HOST), middle piece function (PI) and incubation resistance at 39ºC, 4 h (TR). Majority, post-thaw parameters were higher in those treatments with trehalose, specially MI, AI, PI and TR, indicating that this disaccharide in this conditions improve the morphology and function status of cryopreserved spermatozoa. The extender S with trehalose showed the best results: MI=63,0%; Eo=58%; TR=50%, PI=0,92; AI=75,8%. We conclude that, in that trial, trehalose protects membranes integrity and physiological parameters, and could improve the fertility results in artificial insemination programmes with cryopreserved ram semen. P128 Measurement of sperm capacitation and acrosome reaction - like changes by chlortetracycline test (CTC) can predict the freezability of ram semen Azevedo, HC. 1 *; Bicudo, SD. 2 ; Sousa, DB. 2 ; Maia, MS. 3 ; Rodello, L. 2 ; Sicherle, CC. 2 1Embrapa Coastal Tablelands, Aracaju, Brazil; 2 Faculty of Veterinary Medicine and Anim. Science, São Paulo State University, Brazil; 3 Embrapa Tropical Semi-Arid, Petrolina, Brazil The purpose of this study was to know if the chlortetracycline test (CTC) applied to fresh semen can predict the performance of ram spermatozoa in cryopreservation. Ejaculates of 25 Santa Inês rams were collected and the CTC assay was applied in fresh semen diluting a sample (24 x 10 6 spermatozoa) in 1000 μL of PBS (37 o C) and submitting it to centrifugation (900 g/4´) to remove the seminal plasma. The sperm pellet was then resuspended (150 μL – PBS) and an aliquot (10 μL) was mixed with 10 μL of 1mM CTC (20mM - Tris; 130mM – NaCl; 5mM - L-Cysteine). The mixture was homogenized for 20 seconds and then it was added to 10 μL of 1% of glutaraldehyde solution (2M - Tris). A 10 μL-sample of this suspension was placed on a heated slide (37 o C) and mixed with 10μL of 0.22 M 1,4-diazabicyclo[2.2.2]octane (DABCO – PBS:Glycerol - 1:9). The mixture was covered with coverslips, compressed, sealed and stored at 4 o C in the dark to be evaluated within 1 hour using an epifluorescent microscope under oil (1000x). A total of 100 cells were counted for each slide and distributed into 3 categories: uncapacitated with intact acrosome (F), capacitated with intact acrosome (B) and acrosomal reaction sperm (AR). To frozen semen, the ejaculates were diluted in egg yolk Tris extender (100 x 10 6 spermatozoa/0.25mL), cooled (0.25°C/minute to 5°C - 120 minutes), frozen (-20°C/minute to -120°C) and thawed (42°C/20´´). The frozen-thawed semen was evaluated as for spermatic plasmatic membrane integrity (PMI) by association of propidium iodide (PI – 0.5 mg/mL) and Pisum sativum agglutinin conjugated with fluorescein isothiocyanate (PSA-FITC - 100 g/mL). The rams were grouped in three cryoresistence levels (CRYO) according to general average of PMI (X=22.1%), such as: 1 – inferior (X≤10.3%); 2 – intermediate (10.3%>X≤29.9%) and; 3 – superior (X>29.9%). To all the variables it was used the variance analysis (ANOVA) in a completely randomized design and Tukey method was used to compare the averages (P
16 t h International Congress on Animal Reproduction Poster Abstracts 71 of a 14% CP supplement. The lambs were fed with at libitum access of a 18% PC supplement since 15 th days old until 60 th days old. The data on BW and WW were analyzed by Mixed Model procedures of SAS program. The fixed effects utilized Breed of sire (BS), Breed of ewe (BE), Sex (Sx), Litter size(L) and season(T) nested in BE (T(BE). The random effects were Sire(S) nested in BS and Ewe(E) nested in BE. BW and WW were influenced by BS, L , T(BE) (P 0.05). K lambs ranked first in both measures, 3.42 ± 0.36 kg. and 11.62±1.53 kg. for BW and WW, respectively. Ile(3.06±0.33) were intermediate and DPN (2.96±0.32), DPB (2.87±0.33), BB(2.74±0.35) and PB(2.82±0.33) were last for BW. For WW, Ile(9.95. ±1.44), DPN (9.85±1.40), DPB(9.69±1.44), were intermediate and BB(8.77±1.53) PB(8.45±1.45) were last. Singles lambs (3.69±0.32) had higher weights than double(2.92±0.32) or multiple litter(2.32±0.34) for BW. Also Single lambs (11.21±1.4) were higher than doubles (9.19±1.39) which were higher than multiples (8.76±1.5). We observed that the birth weight is affected by Specialized beef Breed of sire used, without being a serious risk because they are low. Also the weaning weight is improved although it is a trait more influenced by the ewe performance than sire. Notice that the BB and PB ewes have a small frame and multiple litters. Project sponsored by CONACYT-SAGARPA-2004-C01-150 P130 Systemic concentrations of endo- and exogenous FSH in anestrous ewes superovulated with Folltropin ® -V after pretreatment with medroxyprogesterone acetate (MAP)- releasing vaginal sponges and a single dose of estradiol 17β (E2 17β) Bartlewski, PM 1 *; Alexander, BD 2 ; King, WA 1 1Department of Biomedical Sciences, Ontario Veterinary College, University of Guelph, Guelph, Ont., Canada; 2 Department of Farm Animal Production and Health, Faculty of Veterinary Medicine and Animal Science, University of Peradeniya, Peradeniya, Sri Lanka The synchronization of follicular wave emergence with progestin and estradiol 17β (E 2 17β) prior to superovulation in anestrous ewes reduces the variability in ovarian responses by an unknown mechanism. Follicle stimulating hormone (FSH) is a primary promoter of antral follicular growth and maturation, but the relevance of changes in circulating FSH concentrations to the superovulatory performance in ewes remains unknown. This report is comprised of retrospective analyses of serum FSH concentrations in anestrous Rideau Arcott ewes (May-June), which were superovulated with Folltropin ® -V (porcine FSH), with (n=8; treated ewes) or without (n=10; control ewes) a 14-day pretreatment with medroxyprogesterone acetate (MAP)-releasing vaginal sponges (60 mg), and a single i.m. dose of 350 μg E 2 17β given 6 days after insertion of sponges. The superovulatory treatment, started 6 days after E 2 17β injection, consisted of six i.m. doses of Folltropin ® -V (2.5 ml x 1 and 1.25 ml x 5) given twice daily, followed by the bolus injection of GnRH (50 μg i.m.). In order to avoid the effects of interbatch variations in pFSH bioactivity, separate batches of Folltropin ® - V were pooled to prepare sufficient quantities to treat all 18 animals. Serum samples obtained during the superovulatory treatment were analyzed by radioimmunoassays (RIA) for concentrations of ovine (oFSH) and porcine FSH (pFSH), using species-specific standards and primary antibodies. Both gonadotropins have been found to crossreact with the heterospecific antibody; therefore, cross-reactivity was subtracted from all FSH concentrations. The control ewes exceeded E 2 17β-treated animals in serum concentrations of oFSH at 2, 2.5 and 3 days after the first Folltropin ® -V injection (P0.05) between the two groups in pFSH concentrations. The mean ovulation rate and number of recovered embryos did not differ between the two groups of ewes (P>0.05), but the variability in the number of luteal structures and in overall embryo yields was lower in E 2 17β-treated ewes (F test; P
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Reproduction in Domestic Animals - Facultad de Ciencias Veterinarias

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