Reproduction in Domestic Animals - Facultad de Ciencias Veterinarias
Reproduction in Domestic Animals - Facultad de Ciencias Veterinarias
Reproduction in Domestic Animals - Facultad de Ciencias Veterinarias
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16 t h International Congress on Animal <strong>Reproduction</strong><br />
64 Poster Abstracts<br />
<strong>de</strong>f<strong>in</strong>ed follicle classes before and after GnRH application. Ovaries<br />
conta<strong>in</strong><strong>in</strong>g periovulatory follicles or new corpora lutea (CL day 1-2)<br />
were collected at 0h, 4h, 10h, 20h, 25h (follicles) and 60h (CL)<br />
relative to <strong>in</strong>jection of GnRH. The MMP-1 mRNA <strong>in</strong>creased rapidly<br />
4h after GnRH (dur<strong>in</strong>g LH surge) and rema<strong>in</strong>ed high dur<strong>in</strong>g the whole<br />
experimental period. In contrast, the MMP-19 mRNA <strong>in</strong>creased<br />
significantly only after ovulation. The TIMP-1 mRNA <strong>in</strong>creased 4h<br />
after GnRH and aga<strong>in</strong> after ovulation. Transcripts of VEGF isoforms<br />
(VEGF121, 165, 189) were upregulated 4h after GnRH. All VEGF<br />
isoforms and their receptors were upregulated aga<strong>in</strong> after ovulation.<br />
The VEGF pepti<strong>de</strong> content <strong>in</strong> follicular fluid <strong>de</strong>creased 20h followed<br />
by an <strong>in</strong>crease <strong>in</strong> follicle class 25h after GnRH. ANPT-1 mRNA<br />
<strong>de</strong>creased significantly <strong>in</strong> follicles 4h after GnRH. ANPT-2 <strong>de</strong>creased<br />
10h after GnRH and <strong>in</strong> the follicle group around ovulation. Tie-1 and<br />
Tie-2 mRNA expression <strong>de</strong>creased <strong>in</strong> follicle group around ovulation,<br />
with a further <strong>in</strong>crease <strong>in</strong> early CL tissue. It is likely that the <strong>de</strong>crease<br />
of ANPT-1 and therefore the <strong>in</strong>crease of the ANPT-2/ANPT-1 ratio<br />
dur<strong>in</strong>g the LH surge is a basic mechanism of vascular remo<strong>de</strong>ll<strong>in</strong>g <strong>in</strong><br />
follicles dur<strong>in</strong>g periovulation. In conclusion, the temporal expression<br />
pattern of angiogenic factors ANPT and VEGF as well as member of<br />
MMP dur<strong>in</strong>g periovulation suggests them to be important mediators<br />
of the ovulatory process and the early CL formation (angiogenesis) <strong>in</strong><br />
cow.<br />
P109<br />
Changes of the cow’s endometrium, distribution of<br />
growth stimulat<strong>in</strong>g and <strong>de</strong>gradation factors <strong>in</strong><br />
postparturition period<br />
Sematovica, I 1 *; Jemeljanovs, A 1 ; Pilmane, M 2<br />
1Research Institute of Biotechnology and Veter<strong>in</strong>ary Medic<strong>in</strong>e „Sigra”, LUA<br />
Latvia; 2 Institute of Anatomy and Anthropology, RSU, Latvia<br />
Introduction Remarkable morphological and physiological changes<br />
occur <strong>in</strong> the uterus tissue, blood vessels, and nerves dur<strong>in</strong>g the<br />
reproductive cycle and gestation. AIM of the research was to reveal<br />
<strong>in</strong>flammatory factors, neuropepti<strong>de</strong>-conta<strong>in</strong><strong>in</strong>g <strong>in</strong>nervation,<br />
distribution of the growth stimulat<strong>in</strong>g and <strong>de</strong>gradation factors, and the<br />
apoptosis <strong>in</strong> the cows’ endometrium <strong>in</strong> after parturition period.<br />
Materials and methods N<strong>in</strong>e cows were biopsied twice – <strong>in</strong> the first<br />
and the fifth week after parturition. At the Institute of Anatomy and<br />
Anthropology of Riga, Strad<strong>in</strong>s University were performed analyses:<br />
immunohistochemically (IMH) were <strong>de</strong>tected matrix<br />
metalloprote<strong>in</strong>ases type 2 and type 9 (MMP–2 un MMP–9, work<strong>in</strong>g<br />
dilution 1:100, R&D, England), tumor necrosis factor–α (TNF–α,<br />
1:100, Abcam, England), <strong>in</strong>terleuk<strong>in</strong>–10 (IL–10, 1:400, Abcam,<br />
England), vascular endothelial growth factor (VEGF, 1:50,<br />
DakoCytomation, Denmark), nerve growth factor receptors p75<br />
(NGFR p75, w.d. 1:150, DakoCytomation, Denmark) prote<strong>in</strong> gene<br />
product 9.5 (PGP 9.5, 1:1600, DakoCytomation, Denmark), and<br />
TUNEL method was used for <strong>de</strong>tection of apoptosis. Also sta<strong>in</strong><strong>in</strong>g for<br />
hematoxyl<strong>in</strong> and eos<strong>in</strong> was performed for each sample.<br />
Results There were <strong>de</strong>tected a significant <strong>in</strong>crease of the number of<br />
<strong>in</strong>flammatory cells, TNF–α amount, and expression of VEGF, NGFR<br />
p75 (p