Reproduction in Domestic Animals - Facultad de Ciencias Veterinarias
Reproduction in Domestic Animals - Facultad de Ciencias Veterinarias
Reproduction in Domestic Animals - Facultad de Ciencias Veterinarias
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16 t h International Congress on Animal <strong>Reproduction</strong><br />
62 Poster Abstracts<br />
P102<br />
Oestrus synchronization and non return rate <strong>in</strong> dairy<br />
cows treated by two types of prostagland<strong>in</strong><br />
Córdova, A 1 *; Ruiz, CG 1 ; Córdova, MS 2 ; Córdova, CA 3 ; Guerra, JE 4 ; Tapia, B 5<br />
1Departamento <strong>de</strong> Producción Agrícola y Animal. Eco<strong>de</strong>sarrollo <strong>de</strong> la<br />
Producción Animal. Cuerpo Académico: Salud y Bienestar Animal.<br />
Universidad Autónoma Metropolitana-Xochimilco. Calz. <strong>de</strong>l Hueso 1100 Col.<br />
Villa Quietud CPP. 04960, México, D.F.<br />
* aci57@prodigy.net.mx; 2 Laborarotorios Brovel, S.A.<strong>de</strong> C.V.; 3 Becario <strong>de</strong><br />
CONACYT-México. Estudiante <strong>de</strong> Doctorado. Universidad <strong>de</strong> León, España;<br />
4<strong>Facultad</strong> <strong>de</strong> Agronomía. Universidad Autónoma <strong>de</strong> S<strong>in</strong>aloa, México;<br />
5Estudiante <strong>de</strong> Doctorado. <strong>Facultad</strong> <strong>de</strong> Veter<strong>in</strong>aria. Universidad Complutense<br />
<strong>de</strong> Madrid, España<br />
Introduction The implementation of technical and <strong>in</strong>novative<br />
biotechnologies <strong>in</strong> the bov<strong>in</strong>e production, improves the reproductive<br />
efficiency, the oestrus synchronization it is an example; they can be<br />
used similar of prostagland<strong>in</strong>s F2α to shorten the cycle oestrus; that<br />
which improves the oestrus presentation, facilitates the artificial<br />
<strong>in</strong>sem<strong>in</strong>ation (AI), it can <strong>de</strong>crease the return percentage to oestrus;<br />
obta<strong>in</strong><strong>in</strong>g a better handl<strong>in</strong>g of the cluster, s<strong>in</strong>ce more homogeneous<br />
partitions is obta<strong>in</strong>ed <strong>in</strong> the Unit of animal Production.<br />
Objetive Value the effect of the adm<strong>in</strong>istration of two prostagland<strong>in</strong>s<br />
types F2α (Sodium Cloprostenol and right-han<strong>de</strong>d Cloprostenol) on<br />
the synchronization percentage and not return to oestrus <strong>in</strong> dairy<br />
cows.<br />
Methods 20 cows were used <strong>in</strong> production of race Holste<strong>in</strong> Friesian,<br />
which were divi<strong>de</strong>d <strong>in</strong> two groups of 10 animals each one. Before<br />
beg<strong>in</strong>n<strong>in</strong>g the study, the animals were selected, by means of the<br />
ecography use, to verify the ovarian health. In the group 1, 10 cows<br />
were used, to which were adm<strong>in</strong>istered 25 mg via <strong>in</strong>tramuscular of<br />
sodium Cloprostenol. The group 2, with animals, to which were<br />
adm<strong>in</strong>istered 25 mg of right-han<strong>de</strong>d Cloprostenol via <strong>in</strong>tramuscular.<br />
The oestrus <strong>de</strong>tection was carried out realized for observation of<br />
characteristic cl<strong>in</strong>ical signs and AI was carried out by means of the<br />
method AM/PM. The not return to the oestrus it was verified to the 21<br />
days after the AI.<br />
Results The results of oestrus synchronization for the first group<br />
(sodium Cloprostenol) they were from 10% to the 12 hours, 20% at<br />
the 24 hours, 40% to the 48 hours and 30% at the 72 hours after the<br />
adm<strong>in</strong>istration of the prostagland<strong>in</strong>. The group 2 (right-han<strong>de</strong>d<br />
Cloprostenol), the synchronization percentage was from 20% to the<br />
48 hours, 10% to the 56 hours and 70% at the 72 hours. In both<br />
groups 90% was obta<strong>in</strong>ed of not return to oestrus.<br />
Conclusion The adm<strong>in</strong>istration of sodium Cloprostenol could<br />
represent an alternative to be used <strong>in</strong> synchronization programs and<br />
not return to oestrus <strong>in</strong> dairy cows <strong>in</strong> production.<br />
P103<br />
Effect of body condition on uter<strong>in</strong>e <strong>in</strong>volution, ovarian<br />
activity and ovarian IGF-1 receptor expression <strong>in</strong> dual<br />
purpose cattle dur<strong>in</strong>g the postpartum period un<strong>de</strong>r<br />
tropical conditions<br />
Ruiz, A 1 *; Dom<strong>in</strong>guez, C 3 ; Mart<strong>in</strong>ez, N 2 ; Perez, R 3 ; P<strong>in</strong>to, L 2 ; Drescher, K 2 ;<br />
Rojas, J 1 Fernan<strong>de</strong>z, A 1<br />
1<strong>Facultad</strong> <strong>de</strong> <strong>Ciencias</strong> Veter<strong>in</strong>arias, Universidad Central <strong>de</strong> Venezuela,<br />
Venezuela; 2 <strong>Facultad</strong> <strong>de</strong> Agronomía, Universidad Central <strong>de</strong> Venezuela,<br />
Venezuela; 3 Instituto para el Desarrollo Sostenible <strong>de</strong> Sistemas<br />
Agroambientales, Universidad; Experimental Rómulo Gallego, Venezuela<br />
Differential responses on the reproductive performance of cattle,<br />
<strong>de</strong>pend<strong>in</strong>g on the body condition (BC) at calv<strong>in</strong>g and dur<strong>in</strong>g the<br />
postpartum period, have been shown. Insul<strong>in</strong> like growth factors<br />
(IGFs) act modulat<strong>in</strong>g gonadotroph<strong>in</strong> action at a cellular level. Eight<br />
(8) crossbred (Bos taurus x Bos <strong>in</strong>dicus) dual purpose cows were used<br />
and assigned randomly to one of two treatments: T1, low body<br />
condition (BC < 2.5) and low feed level (LF, 85% of the nutritional<br />
requirements) or high feed level (HF, 115% of the nutritional<br />
requirements); T2, high BC (> 2.5) and LF or HF levels. Reproductive<br />
activity and uter<strong>in</strong>e <strong>in</strong>volution (UI) were evaluated once a week by<br />
means of transrectal palpation and ultrasound (Aloka SSD 900 Co.<br />
Ltd., Tokyo, Japan) from 15 to 45 days post-partum (DPP) us<strong>in</strong>g a 7.5<br />
MHz l<strong>in</strong>ear probe. Plasma progesterone (P 4 ) levels were measured by<br />
RIA (DSL, Diagnostic Systems Lab, Inc., TX, USA) at 3, 15, 22, 33,<br />
37, and 45 DPP. Uter<strong>in</strong>e <strong>in</strong>volution, characteristics of cervical mucus<br />
(CCM), symmetry of uter<strong>in</strong>e horns (SUH), uter<strong>in</strong>e position (UP), and<br />
cervix diameter (CD) were studied. Ovarian structures (corpora lutea<br />
and follicles) were evaluated by ultrasound. Ovarian follicles were<br />
classified accord<strong>in</strong>g to their diameters: Class 1 (≤ 5 mm); Class 2 (6-9<br />
mm) and; Class 3 (≥10 mm). At 45 DPP, ovaries were collected<br />
with<strong>in</strong> 30 m<strong>in</strong> after slaughter. One ovary was used to evaluate the<br />
morphology of oocytes, which were categorized as follows: type A<br />
(oocyte with the presence of a clear and compact cumulus oophurus<br />
and translucent ooplasm); type B (oocyte with dark and compact<br />
cumulus oophurus and dark ooplasm) and; type C (oocyte with dark<br />
and expan<strong>de</strong>d cumulus oophurus and dark ooplasm). The other ovary<br />
was used to study the IGF-1 receptor expression by Western blot<br />
analysis. Results were analyzed by the SPPS (version 10), us<strong>in</strong>g a<br />
non-parametric correlation and the Kruskal and Wallis tests. The nonparametric<br />
ANOVA showed that BC at calv<strong>in</strong>g affected type C<br />
oocytes (P< 0.01). A similar result was found by the correlation<br />
analysis (-.816*). There was not any effect of BC at calv<strong>in</strong>g on UI and<br />
follicle classes. Plasma P 4 levels were affected by BC at calv<strong>in</strong>g from<br />
33 DPP. The ovarian IGF-1 receptor showed a higher expression <strong>in</strong><br />
animals un<strong>de</strong>r T1, compared with those animals <strong>in</strong> T2. The results<br />
suggest that BC at calv<strong>in</strong>g is one of the factors <strong>in</strong>volved <strong>in</strong> the<br />
resumption of the reproductive activity dur<strong>in</strong>g the postpartum period<br />
un<strong>de</strong>r tropical conditions. Also, it appears that IGFs act as modulators<br />
of gonadotroph<strong>in</strong> functions at the ovary level <strong>in</strong> this species.<br />
P104<br />
Ultrasonographic <strong>de</strong>tection of embryo and its<br />
<strong>de</strong>velopment <strong>in</strong> crossbred cows<br />
Sahatpure,SK.*, Pardhi.SM. & Khillare,KP.<br />
Postgraduate Institute of Veter<strong>in</strong>ary &Animal Science Akola Maharashtra<br />
India-444104 (Un<strong>de</strong>r Maharashtra Animal &Fishery Science University<br />
Nagpur )<br />
Ultrasound was used for the <strong>de</strong>tection of embryo and its <strong>de</strong>velopment<br />
<strong>in</strong> 6 cyclic crossbred cows from day 20 to day 60 after breed<strong>in</strong>g.<br />
In the present study, synchronization of estrus was carried out by<br />
adm<strong>in</strong>istrat<strong>in</strong>g Lutalyse (PGF 2 α). The number of cows respon<strong>de</strong>d to<br />
treatment was 100 per cent and the average time required for onset of<br />
estrus was 59.83 ± 1.14 hours.<br />
In the present study all artificially <strong>in</strong>sem<strong>in</strong>ated crossbred Cows were<br />
scanned with diagnostic ultrasound scanner equipped with a l<strong>in</strong>ear<br />
array, 7.5 MHz transducer <strong>de</strong>signed for <strong>in</strong>trarectal placement. All<br />
animals were scanned ultrasonographically with an <strong>in</strong>terval of 5 days<br />
from day 20 to day 60 after breed<strong>in</strong>g.<br />
The embryonic vesicle was first visible on day 20. The embryo proper<br />
was first time observed <strong>in</strong> all cows on mean day 25. The heart beats of<br />
the embryo proper was <strong>de</strong>tected on day 27.5 ± 1.44. The allontois,<br />
amnion, forelimbs, h<strong>in</strong>d limbs and optic area were first i<strong>de</strong>ntified on<br />
day 28.75 ± 1.25; 30; 32.5 ± 1.44; 33.75 ± 1.25 and 28.75 ± 2.39,<br />
respectively. The sp<strong>in</strong>al cord, ribs and fetal movements were observed<br />
on average day of 47.5 ± 2.39; 55 and 53.75 ± 1.25, respectively.<br />
The accuracy of early pregnancy diagnosis <strong>in</strong> means of<br />
ultrasonography were 66.66, 83.33 and 100 per cent on day 20 th , 25 th<br />
and 30 th day of gestation, respectively, whereas, sensitivity and<br />
specificity of ultrasound pregnancy diagnosis was 75 and 50 per cent<br />
on day 20 respectively and 100 per cent on day 25 and 30 of gestation.<br />
P105<br />
Frequency of <strong>de</strong>tection of estrous behavior<br />
accompany<strong>in</strong>g early postpartum ovulations <strong>in</strong> fertile dairy<br />
cows<br />
Sakaguchi, M<br />
National Agricultural Research Center for Hokkaido Region, NARO<br />
Introduction Increased milk yield <strong>in</strong> dairy cattle causes a cont<strong>in</strong>uous<br />
and serious <strong>de</strong>cl<strong>in</strong>e <strong>in</strong> their fertility. Many factors such as genetic