Reproduction in Domestic Animals - Facultad de Ciencias Veterinarias

16 t h International Congress on Animal Reproduction
62 Poster Abstracts
P102
Oestrus synchronization and non return rate in dairy
cows treated by two types of prostaglandin
Córdova, A 1 *; Ruiz, CG 1 ; Córdova, MS 2 ; Córdova, CA 3 ; Guerra, JE 4 ; Tapia, B 5
1Departamento de Producción Agrícola y Animal. Ecodesarrollo de la
Producción Animal. Cuerpo Académico: Salud y Bienestar Animal.
Universidad Autónoma Metropolitana-Xochimilco. Calz. del Hueso 1100 Col.
Villa Quietud CPP. 04960, México, D.F.
* aci57@prodigy.net.mx; 2 Laborarotorios Brovel, S.A.de C.V.; 3 Becario de
CONACYT-México. Estudiante de Doctorado. Universidad de León, España;
4Facultad de Agronomía. Universidad Autónoma de Sinaloa, México;
5Estudiante de Doctorado. Facultad de Veterinaria. Universidad Complutense
de Madrid, España
Introduction The implementation of technical and innovative
biotechnologies in the bovine production, improves the reproductive
efficiency, the oestrus synchronization it is an example; they can be
used similar of prostaglandins F2α to shorten the cycle oestrus; that
which improves the oestrus presentation, facilitates the artificial
insemination (AI), it can decrease the return percentage to oestrus;
obtaining a better handling of the cluster, since more homogeneous
partitions is obtained in the Unit of animal Production.
Objetive Value the effect of the administration of two prostaglandins
types F2α (Sodium Cloprostenol and right-handed Cloprostenol) on
the synchronization percentage and not return to oestrus in dairy
cows.
Methods 20 cows were used in production of race Holstein Friesian,
which were divided in two groups of 10 animals each one. Before
beginning the study, the animals were selected, by means of the
ecography use, to verify the ovarian health. In the group 1, 10 cows
were used, to which were administered 25 mg via intramuscular of
sodium Cloprostenol. The group 2, with animals, to which were
administered 25 mg of right-handed Cloprostenol via intramuscular.
The oestrus detection was carried out realized for observation of
characteristic clinical signs and AI was carried out by means of the
method AM/PM. The not return to the oestrus it was verified to the 21
days after the AI.
Results The results of oestrus synchronization for the first group
(sodium Cloprostenol) they were from 10% to the 12 hours, 20% at
the 24 hours, 40% to the 48 hours and 30% at the 72 hours after the
administration of the prostaglandin. The group 2 (right-handed
Cloprostenol), the synchronization percentage was from 20% to the
48 hours, 10% to the 56 hours and 70% at the 72 hours. In both
groups 90% was obtained of not return to oestrus.
Conclusion The administration of sodium Cloprostenol could
represent an alternative to be used in synchronization programs and
not return to oestrus in dairy cows in production.
P103
Effect of body condition on uterine involution, ovarian
activity and ovarian IGF-1 receptor expression in dual
purpose cattle during the postpartum period under
tropical conditions
Ruiz, A 1 *; Dominguez, C 3 ; Martinez, N 2 ; Perez, R 3 ; Pinto, L 2 ; Drescher, K 2 ;
Rojas, J 1 Fernandez, A 1
1Facultad de Ciencias Veterinarias, Universidad Central de Venezuela,
Venezuela; 2 Facultad de Agronomía, Universidad Central de Venezuela,
Venezuela; 3 Instituto para el Desarrollo Sostenible de Sistemas
Agroambientales, Universidad; Experimental Rómulo Gallego, Venezuela
Differential responses on the reproductive performance of cattle,
depending on the body condition (BC) at calving and during the
postpartum period, have been shown. Insulin like growth factors
(IGFs) act modulating gonadotrophin action at a cellular level. Eight
(8) crossbred (Bos taurus x Bos indicus) dual purpose cows were used
and assigned randomly to one of two treatments: T1, low body
condition (BC < 2.5) and low feed level (LF, 85% of the nutritional
requirements) or high feed level (HF, 115% of the nutritional
requirements); T2, high BC (> 2.5) and LF or HF levels. Reproductive
activity and uterine involution (UI) were evaluated once a week by
means of transrectal palpation and ultrasound (Aloka SSD 900 Co.
Ltd., Tokyo, Japan) from 15 to 45 days post-partum (DPP) using a 7.5
MHz linear probe. Plasma progesterone (P 4 ) levels were measured by
RIA (DSL, Diagnostic Systems Lab, Inc., TX, USA) at 3, 15, 22, 33,
37, and 45 DPP. Uterine involution, characteristics of cervical mucus
(CCM), symmetry of uterine horns (SUH), uterine position (UP), and
cervix diameter (CD) were studied. Ovarian structures (corpora lutea
and follicles) were evaluated by ultrasound. Ovarian follicles were
classified according to their diameters: Class 1 (≤ 5 mm); Class 2 (6-9
mm) and; Class 3 (≥10 mm). At 45 DPP, ovaries were collected
within 30 min after slaughter. One ovary was used to evaluate the
morphology of oocytes, which were categorized as follows: type A
(oocyte with the presence of a clear and compact cumulus oophurus
and translucent ooplasm); type B (oocyte with dark and compact
cumulus oophurus and dark ooplasm) and; type C (oocyte with dark
and expanded cumulus oophurus and dark ooplasm). The other ovary
was used to study the IGF-1 receptor expression by Western blot
analysis. Results were analyzed by the SPPS (version 10), using a
non-parametric correlation and the Kruskal and Wallis tests. The nonparametric
ANOVA showed that BC at calving affected type C
oocytes (P< 0.01). A similar result was found by the correlation
analysis (-.816*). There was not any effect of BC at calving on UI and
follicle classes. Plasma P 4 levels were affected by BC at calving from
33 DPP. The ovarian IGF-1 receptor showed a higher expression in
animals under T1, compared with those animals in T2. The results
suggest that BC at calving is one of the factors involved in the
resumption of the reproductive activity during the postpartum period
under tropical conditions. Also, it appears that IGFs act as modulators
of gonadotrophin functions at the ovary level in this species.
P104
Ultrasonographic detection of embryo and its
development in crossbred cows
Sahatpure,SK.*, Pardhi.SM. & Khillare,KP.
Postgraduate Institute of Veterinary &Animal Science Akola Maharashtra
India-444104 (Under Maharashtra Animal &Fishery Science University
Nagpur )
Ultrasound was used for the detection of embryo and its development
in 6 cyclic crossbred cows from day 20 to day 60 after breeding.
In the present study, synchronization of estrus was carried out by
administrating Lutalyse (PGF 2 α). The number of cows responded to
treatment was 100 per cent and the average time required for onset of
estrus was 59.83 ± 1.14 hours.
In the present study all artificially inseminated crossbred Cows were
scanned with diagnostic ultrasound scanner equipped with a linear
array, 7.5 MHz transducer designed for intrarectal placement. All
animals were scanned ultrasonographically with an interval of 5 days
from day 20 to day 60 after breeding.
The embryonic vesicle was first visible on day 20. The embryo proper
was first time observed in all cows on mean day 25. The heart beats of
the embryo proper was detected on day 27.5 ± 1.44. The allontois,
amnion, forelimbs, hind limbs and optic area were first identified on
day 28.75 ± 1.25; 30; 32.5 ± 1.44; 33.75 ± 1.25 and 28.75 ± 2.39,
respectively. The spinal cord, ribs and fetal movements were observed
on average day of 47.5 ± 2.39; 55 and 53.75 ± 1.25, respectively.
The accuracy of early pregnancy diagnosis in means of
ultrasonography were 66.66, 83.33 and 100 per cent on day 20 th , 25 th
and 30 th day of gestation, respectively, whereas, sensitivity and
specificity of ultrasound pregnancy diagnosis was 75 and 50 per cent
on day 20 respectively and 100 per cent on day 25 and 30 of gestation.
P105
Frequency of detection of estrous behavior
accompanying early postpartum ovulations in fertile dairy
cows
Sakaguchi, M
National Agricultural Research Center for Hokkaido Region, NARO
Introduction Increased milk yield in dairy cattle causes a continuous
and serious decline in their fertility. Many factors such as genetic