Reproduction in Domestic Animals - Facultad de Ciencias Veterinarias
Reproduction in Domestic Animals - Facultad de Ciencias Veterinarias
Reproduction in Domestic Animals - Facultad de Ciencias Veterinarias
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16 t h International Congress on Animal <strong>Reproduction</strong><br />
Poster Abstracts 57<br />
NEFA <strong>in</strong> HPC (0,44 ± 0,08 mmol/l) opposite LPC (0,23 ± 0,11<br />
mmol/l) ((P0,05). We recor<strong>de</strong>d no significantly changes of<br />
concentrations of TG, urea and AST between groups dur<strong>in</strong>g ante and<br />
postpartum period. Dynamic changes IGF–I, NEFA and glucose <strong>in</strong><br />
plasma or serum may help predict and evaluate relationship between<br />
milk yield, nutrition and subsequent reproductive status of high<br />
produc<strong>in</strong>g cows. (Fun<strong>de</strong>d by VEGA 1/3484/06 and AV 4/0009/07)<br />
P088<br />
Effect of restricted suckl<strong>in</strong>g on superovulatory response<br />
and reproductive performance <strong>in</strong> early postpartum<br />
Japanese Black cows<br />
Oshima, K 1 *; Ochiai, Y 1 ; Pradhan, R 2 ; Kojima, T 1 ; Yamamoto, N 1<br />
1National Agricultural Research Center for Western Region, Oda, Shimane,<br />
Japan; 2 Graduate School of Hiroshima University, Higashi-Hiroshima,<br />
Hiroshima, Japan<br />
Factors such as suckl<strong>in</strong>g and nutrition affect the reproductive<br />
performance after parturition <strong>in</strong> cows. The objective of this study was<br />
to <strong>in</strong>vestigate the effect of restricted suckl<strong>in</strong>g on the superovulatory<br />
response and subsequent reproductive performance <strong>in</strong> early<br />
postpartum Japanese Black cows. Fifty cows were used <strong>in</strong> this study.<br />
Dur<strong>in</strong>g the postpartum period, these cows were fed 100% of the<br />
estimated daily nutrient requirements accord<strong>in</strong>g to the Japanese<br />
Feed<strong>in</strong>g Standard for Beef Cattle (2000). Body weights of cows and<br />
calves were measured twice monthly. The average daily ga<strong>in</strong> (ADG)<br />
was calculated for each month. At 7 days postpartum, the cows were<br />
classified <strong>in</strong>to 2 groups: (1) cont<strong>in</strong>uous access of calves to them from<br />
birth to wean<strong>in</strong>g at 3 months postpartum (ad libitum suckl<strong>in</strong>g; n = 21)<br />
and (2) twice daily suckl<strong>in</strong>g by calves that were penned adjacent to<br />
them (restricted suckl<strong>in</strong>g; n = 29). All cows received a controlled<br />
<strong>in</strong>ternal drug releas<strong>in</strong>g <strong>de</strong>vice (Easi-Breed; InterAg, Hamilton, New<br />
Zealand) at 40 days postpartum and were subsequently<br />
superstimulated with a total dose of 20 armour units FSH (Antr<strong>in</strong> 40;<br />
Kawasaki-Mitaka, Kanagawa, Japan) twice daily, with gradually<br />
<strong>de</strong>creas<strong>in</strong>g doses from day 45 until day 47. Embryos were<br />
nonsurgically collected 7 to 8 days after estrus. The ovaries were<br />
exam<strong>in</strong>ed by ultrasonography and the number of CL and rema<strong>in</strong><strong>in</strong>g<br />
follicles (RF) were counted. After uter<strong>in</strong>e flush<strong>in</strong>g, the cows were reemployed<br />
for reproductive purposes. The <strong>in</strong>tervals to first estrus and<br />
conception after flush<strong>in</strong>g and days open were exam<strong>in</strong>ed. Data were<br />
analyzed by Stu<strong>de</strong>nt’s t-test. ADG of cows <strong>in</strong> the second month<br />
postpartum <strong>in</strong> the ad libitum suckl<strong>in</strong>g group was significantly smaller<br />
than that <strong>in</strong> the restricted suckl<strong>in</strong>g group (P < 0.05). There were no<br />
significant differences between the ad libitum and restricted suckl<strong>in</strong>g<br />
groups <strong>in</strong> the number of CL (20.7 ± 10.0 vs.17.0 ± 9.2), RF (4.7 ± 3.6<br />
vs. 5.9 ± 7.0), recovered ova or embryos (10.6 ± 7.4 vs. 11.1 ± 8.8),<br />
and transferable and freezable embryos (6.4 ± 5.4 and 5.2 ± 5.0 vs. 7.4<br />
± 7.0 and 6.2 ± 6.9). In contrast, the <strong>in</strong>tervals to first estrus and<br />
conception after flush<strong>in</strong>g and days open <strong>in</strong> the restricted suckl<strong>in</strong>g<br />
group were significantly shorter (P < 0.05) than those <strong>in</strong> the ad libitum<br />
suckl<strong>in</strong>g group (9.0 ± 5.6, 27.1 ± 27.5, and 83.7 ± 27.5 vs. 28.0 ±<br />
23.6, 44.2 ± 30.1, and 101.0 ± 30.2). These results suggest that<br />
restricted suckl<strong>in</strong>g <strong>in</strong> early postpartum Japanese Black cows does not<br />
affect the superovulatory response and embryo quality; however, it<br />
does affect their reproductive performance after flush<strong>in</strong>g.<br />
P089<br />
Integration of CIDR <strong>in</strong> timed artificial <strong>in</strong>sem<strong>in</strong>ation<br />
protocol <strong>in</strong> lactat<strong>in</strong>g dairy cows<br />
Pancarci, SM 1 *, Gurbulak, K 2 , Gungor, O 1 , Orkun Demiral, O 3 , Thatcher, WW 4<br />
1Department of Obstetrics,Gynecology & <strong>Reproduction</strong>, Faculty of Veter<strong>in</strong>ary<br />
Medic<strong>in</strong>e, Kafkas University, Turkey; 2 Department of Obstetrics,Gynecology &<br />
<strong>Reproduction</strong>, Faculty of Veter<strong>in</strong>ary Medic<strong>in</strong>e, Erciyes University,<br />
Turkey; 3 Department of Artificial Insem<strong>in</strong>ation and <strong>Reproduction</strong>, Faculty of<br />
Veter<strong>in</strong>ary Medic<strong>in</strong>e, Erciyes University, Turkey 4 Department of Animal<br />
Sciences, University of Florida, United States<br />
The objective of this study was to <strong>in</strong>vestigate the use of a controlled<br />
<strong>in</strong>ternal drug [progesterone] release (CIDR) <strong>de</strong>vice <strong>in</strong>serted<br />
concurrently with or the day after <strong>in</strong>itiation of Ovsynch/TAI protocol.<br />
Lactat<strong>in</strong>g Holste<strong>in</strong> dairy cows <strong>in</strong> central Turkey (n=162) were<br />
assigned to receive the Ovsynch protocol (OVSYNCH;<br />
synchronization of ovulation by <strong>in</strong>ject<strong>in</strong>g GnRH 7 d before and 56h<br />
after PGF2α, followed by one fixed-time AI [TAI] 16 to 18 h after the<br />
second GnRH <strong>in</strong>jection; n=49], Ovsynch plus a CIDR <strong>in</strong>sert for 7 d,<br />
beg<strong>in</strong>n<strong>in</strong>g at the first GnRH <strong>in</strong>jection (OVSYNCH + CIDR7; n=63)<br />
or Ovsynch plus a CIDR <strong>in</strong>sert for 6 d, beg<strong>in</strong>n<strong>in</strong>g the day after the<br />
first GnRH <strong>in</strong>jection (OVSYNCH + CIDR6; n=50). All cows used <strong>in</strong><br />
this study were restricted to not hav<strong>in</strong>g a <strong>de</strong>tected estrus prior to TAI<br />
and had a palpable follicle at TAI. Pregnancies were diagnosed with<br />
transrectal ultrasonography and palpation per rectum 32 and 45-60<br />
days after TAI, respectively. Pregnancy rates at 32 days after TAI<br />
were 30.6% (15/49), 39.7% (25/63) and 54.0% (27/50) <strong>in</strong><br />
OVSYNCH, OVSYNCH + CIDR7 and OVSYNCH + CIDR6 groups,<br />
respectively. The stepwise logistic regression procedure <strong>in</strong>dicated that<br />
cows <strong>in</strong> OVSYNCH + CIDR7 group had 0.56 (0.26-1.19) times less<br />
chance to get pregnant than those <strong>in</strong> OVSYNCH + CIDR6 group at 32<br />
days after TAI. Similarly, cows <strong>in</strong> OVSYNCH group had 0.38 (0.17-<br />
0.86) times less chance to get pregnant than those <strong>in</strong> OVSYNCH +<br />
CIDR6 group at 32 days after TAI. Pregnancy rates 45-60 days after<br />
TAI were 14.3% (7/49), 20.6% (13/63) and 38.0% (19/50) <strong>in</strong><br />
OVSYNCH, OVSYNCH + CIDR7 and OVSYNCH + CIDR6 groups,<br />
respectively. Significant differences (P