Reproduction in Domestic Animals - Facultad de Ciencias Veterinarias

16 t h International Congress on Animal Reproduction
Poster Abstracts 57
NEFA in HPC (0,44 ± 0,08 mmol/l) opposite LPC (0,23 ± 0,11
mmol/l) ((P0,05). We recorded no significantly changes of
concentrations of TG, urea and AST between groups during ante and
postpartum period. Dynamic changes IGF–I, NEFA and glucose in
plasma or serum may help predict and evaluate relationship between
milk yield, nutrition and subsequent reproductive status of high
producing cows. (Funded by VEGA 1/3484/06 and AV 4/0009/07)
P088
Effect of restricted suckling on superovulatory response
and reproductive performance in early postpartum
Japanese Black cows
Oshima, K 1 *; Ochiai, Y 1 ; Pradhan, R 2 ; Kojima, T 1 ; Yamamoto, N 1
1National Agricultural Research Center for Western Region, Oda, Shimane,
Japan; 2 Graduate School of Hiroshima University, Higashi-Hiroshima,
Hiroshima, Japan
Factors such as suckling and nutrition affect the reproductive
performance after parturition in cows. The objective of this study was
to investigate the effect of restricted suckling on the superovulatory
response and subsequent reproductive performance in early
postpartum Japanese Black cows. Fifty cows were used in this study.
During the postpartum period, these cows were fed 100% of the
estimated daily nutrient requirements according to the Japanese
Feeding Standard for Beef Cattle (2000). Body weights of cows and
calves were measured twice monthly. The average daily gain (ADG)
was calculated for each month. At 7 days postpartum, the cows were
classified into 2 groups: (1) continuous access of calves to them from
birth to weaning at 3 months postpartum (ad libitum suckling; n = 21)
and (2) twice daily suckling by calves that were penned adjacent to
them (restricted suckling; n = 29). All cows received a controlled
internal drug releasing device (Easi-Breed; InterAg, Hamilton, New
Zealand) at 40 days postpartum and were subsequently
superstimulated with a total dose of 20 armour units FSH (Antrin 40;
Kawasaki-Mitaka, Kanagawa, Japan) twice daily, with gradually
decreasing doses from day 45 until day 47. Embryos were
nonsurgically collected 7 to 8 days after estrus. The ovaries were
examined by ultrasonography and the number of CL and remaining
follicles (RF) were counted. After uterine flushing, the cows were reemployed
for reproductive purposes. The intervals to first estrus and
conception after flushing and days open were examined. Data were
analyzed by Student’s t-test. ADG of cows in the second month
postpartum in the ad libitum suckling group was significantly smaller
than that in the restricted suckling group (P < 0.05). There were no
significant differences between the ad libitum and restricted suckling
groups in the number of CL (20.7 ± 10.0 vs.17.0 ± 9.2), RF (4.7 ± 3.6
vs. 5.9 ± 7.0), recovered ova or embryos (10.6 ± 7.4 vs. 11.1 ± 8.8),
and transferable and freezable embryos (6.4 ± 5.4 and 5.2 ± 5.0 vs. 7.4
± 7.0 and 6.2 ± 6.9). In contrast, the intervals to first estrus and
conception after flushing and days open in the restricted suckling
group were significantly shorter (P < 0.05) than those in the ad libitum
suckling group (9.0 ± 5.6, 27.1 ± 27.5, and 83.7 ± 27.5 vs. 28.0 ±
23.6, 44.2 ± 30.1, and 101.0 ± 30.2). These results suggest that
restricted suckling in early postpartum Japanese Black cows does not
affect the superovulatory response and embryo quality; however, it
does affect their reproductive performance after flushing.
P089
Integration of CIDR in timed artificial insemination
protocol in lactating dairy cows
Pancarci, SM 1 *, Gurbulak, K 2 , Gungor, O 1 , Orkun Demiral, O 3 , Thatcher, WW 4
1Department of Obstetrics,Gynecology & Reproduction, Faculty of Veterinary
Medicine, Kafkas University, Turkey; 2 Department of Obstetrics,Gynecology &
Reproduction, Faculty of Veterinary Medicine, Erciyes University,
Turkey; 3 Department of Artificial Insemination and Reproduction, Faculty of
Veterinary Medicine, Erciyes University, Turkey 4 Department of Animal
Sciences, University of Florida, United States
The objective of this study was to investigate the use of a controlled
internal drug [progesterone] release (CIDR) device inserted
concurrently with or the day after initiation of Ovsynch/TAI protocol.
Lactating Holstein dairy cows in central Turkey (n=162) were
assigned to receive the Ovsynch protocol (OVSYNCH;
synchronization of ovulation by injecting GnRH 7 d before and 56h
after PGF2α, followed by one fixed-time AI [TAI] 16 to 18 h after the
second GnRH injection; n=49], Ovsynch plus a CIDR insert for 7 d,
beginning at the first GnRH injection (OVSYNCH + CIDR7; n=63)
or Ovsynch plus a CIDR insert for 6 d, beginning the day after the
first GnRH injection (OVSYNCH + CIDR6; n=50). All cows used in
this study were restricted to not having a detected estrus prior to TAI
and had a palpable follicle at TAI. Pregnancies were diagnosed with
transrectal ultrasonography and palpation per rectum 32 and 45-60
days after TAI, respectively. Pregnancy rates at 32 days after TAI
were 30.6% (15/49), 39.7% (25/63) and 54.0% (27/50) in
OVSYNCH, OVSYNCH + CIDR7 and OVSYNCH + CIDR6 groups,
respectively. The stepwise logistic regression procedure indicated that
cows in OVSYNCH + CIDR7 group had 0.56 (0.26-1.19) times less
chance to get pregnant than those in OVSYNCH + CIDR6 group at 32
days after TAI. Similarly, cows in OVSYNCH group had 0.38 (0.17-
0.86) times less chance to get pregnant than those in OVSYNCH +
CIDR6 group at 32 days after TAI. Pregnancy rates 45-60 days after
TAI were 14.3% (7/49), 20.6% (13/63) and 38.0% (19/50) in
OVSYNCH, OVSYNCH + CIDR7 and OVSYNCH + CIDR6 groups,
respectively. Significant differences (P