Reproduction in Domestic Animals - Facultad de Ciencias Veterinarias

More documents

Recommendations

Info

$(Microsoft PowerPoint - Lecci\363n 2_07.ppt)$

16 t h International Congress on Animal Reproduction 54 Poster Abstracts P079 Evaluation of oestrus detection efficacy and accuracy by three methods in a confinement or pasture management system with Holstein–Friesian cows Mee, JF 1 *; Palmer, MA 2 ; Olmos, G 1,3 ; Boyle, LA 1 1Dairy Production Department, Moorepark Dairy Production Research Centre, Teagasc, Ireland; 2 Royal (Dick) School of Veterinary Studies, University of Edinburgh, Scotland; 3 School of Agriculture, Food Science and Veterinary Medicine, National University of Ireland The objective of this experiment was to compare the efficacy of three methods of oestrus detection [visual observation (VO), tail paint (TP) and radiotelemetry-HeatWatch® (HW)] in two management systems [cubicle housing with a total mixed ration (HOUSED) and rotational pasture with concentrate supplementation (GRASS)]. The 46 randomly allocated and blocked, spring-calving Holstein-Friesian cows were monitored by the three oestrus detection methods simultaneously from ten days postcalving for nine weeks on the same farm. The occurrence of nine selected behaviours associated with oestrus was also recorded during the thrice daily 20 minute visual observation sessions. Thrice weekly milk sampling for progesterone analysis (EIA) was used to determine the dates of true standing oestrus events (oestrus detection accuracy). Data were analysed by proc Frequency, Genmod, Npar1way, Ttest and Univariate, as appropriate, in SAS. All three detection methods had a higher oestrus detection efficacy in the GRASS (VO 59, TP 65 and HW 69%) compared to the HOUSED treatment (VO 20, TP 26 and HW 37%) (P
16 t h International Congress on Animal Reproduction Poster Abstracts 55 P082 An abnormal gonadal development in bovine XY female may be caused by the formation of iso-Y chromosome during meiosis Miyake, YI 1 *, Matsui, M 1 , Moriyama, C 2 , Kamimura, S 2 1Department of Clinical Veterinary Science, Obihiro University of Agriculture and Veterinary Medicine, Japan; 2 Department of Veterinary Science, University of Miyazaki, Japan Introduction It has been reported that some types of chromosomal aberration relate with some types of abnormal development of genital organs in domestic animals, especially in cattle. Those animals with absolute or relative sterility are the object of that research; 1) to eliminate them from herd, and 2) to understand the abnormal conditions. Here, we would like to report some results about the cases of bovine XY female. Results 1. Clinical findings: A total of 9 cases were composed of Holstein (n=7), Japanese Black (n=1) and Jersey (n=1). Although all cases showed a female type in appearance, they did not show any estrous symptoms until 23 month old after the birth. Genital organs The ovaries and uterus were very small. Moreover, follicles and corpus luteum were not observed in ovaries. However, none of male reproductive organs were detected after slaughter. Chromosomal analysis The cells derived from blood, skin, spleen and kidney cultures showed 60,XY only. In the one smallest metacentric Y chromosomes, the only centromere was darkly stained by G-banded staining, although the short arm was darkly stained in normal Y chromosome. Detention of Sry gene by PCR method In the normal bull (60,XY), the Sry gene was detected by PCR method. On the other hand, the Sry gene was not detected in normal female (60,XX) and in the present cases (60,XY). Analysis of structure of Y chromosome by FISH method In Y chromosome from normal bull, one signal of the bovine Y-specific primers, BC 1.2 was hybridized to the distal area of short arm, but in Y chromosome from the present cases, two signals of BC 1.2 were hybridized to same position of both arms. In Y chromosome from normal bull, one signal of another bovine Y-specific primers, btDYZ- 1 was hybridized in close proximity to the centromere of short arm, while in Y chromosome from the present cases, two signals of btDYZ-1 were hybridized to the same position of both arms. 6. Endocrinological measurements by RIA: The high levels of peripheral FSH and LH, and low levels of peripheral P4, E2, T and irinhibin were maintained during 23 days. Furthermore, the cyclic changes of hormones as normal female were not observed. Conclusion It was assumed that the abnormal Y chromosome from the present cases is iso-Y chromosome composed of double short arms which may be formed during spermatogenesis. Furthermore, a set of Y chromosome without Sry gene and X chromosome will induce gonadal dysgenensis and also abnormal endocrinologicalte status in the present bovine XY female. P083 Ovarian structures and ovulation rate following GnRH and progesterone treatment in anestrous Bos indicus cows Montiel, F 1 *; Lamothe, C 1 ; Severino, V 2 1Faculty of Veterinary Medicine, University of Veracruz, Mexico; 2 Faculty of Veterinary Medicine, Autonomous National University of Mexico, Mexico Introduction Bos indicus cattle predominant in tropical regions exhibit low reproductive efficiency because of prolonged periods of postpartum anestrus, characterized by a pronounced delay of development of dominant follicles and first ovulation. Many treatments used for the re-establishment of postpartum ovarian cyclicity include the use of a vaginal insert impregnated with 1.9 g of progesterone (CIDR®, controlled internal drug-releasing device). In these protocols, GnRH has been used to synchronize follicular wave emergence and ovulation for timed artificial insemination (TAI). We evaluated the effect of treatment with CIDR plus GnRH on the ovarian structures and ovulation rate of postpartum suckled anestrous Bos indicus cows. Methods On days -14, -7 and 0 (day 0=start of treatment), ultrasonography (US) was performed in all cows to confirm anestrus, but with the presence of ovarian follicles ≥10 mm diameter on day 0. On day 0, 68 cows received one of the following treatments: 1) GnRH+CIDR+GnRH (n=15): cows were given i.m. 100 μg of a synthetic GnRH (Gonadorelin) plus one CIDR, which remained in situ 7 days, plus 100 μg of GnRH at 24 h after the CIDR removal; 2) GnRH+CIDR (n=14): 100 μg of GnRH plus one CIDR (7 days in situ); 3) CIDR+GnRH (n=15): one CIDR (7 days in situ) and 100 μg of GnRH at 24 h after its removal; 4) CIDR (n=12): one CIDR (7 days in situ); 5) Control (n=12): no treatment. From days 1 to 7, US was performed daily to characterize changes in the ovarian structures as a result of treatment. From days 8 to 10, US was performed every 6 h to determine the time of ovulation. Results The groups treated with CIDR and GnRH showed greater number of follicles and larger average follicular size, compared to the control group (P
Page 1 and 2:
REPRODUCTION IN DOMESTIC ANIMALS 43
Page 3 and 4:
16th International Congress on Anim
Page 5 and 6: 16 t h International Congress on An
Page 55: 16 t h International Congress on An
Page 107 and 108:
16 t h International Congress on An
Page 109 and 110:
Page 111 and 112:
Page 113 and 114:
Page 115 and 116:
Page 117 and 118:
Page 119 and 120:
Page 121 and 122:
Page 123 and 124:
Page 125 and 126:
Page 127 and 128:
Page 129 and 130:
Page 131 and 132:
Page 133 and 134:
Page 135 and 136:
Page 137 and 138:
Page 139 and 140:
Page 141 and 142:
Page 143 and 144:
Page 145 and 146:
Page 147 and 148:
Page 149 and 150:
Page 151 and 152:
Page 153 and 154:
Page 155 and 156:
Page 157 and 158:
Page 159 and 160:
Page 161 and 162:
Page 163 and 164:
Page 165 and 166:
Page 167 and 168:
Page 169 and 170:
Page 171 and 172:
Page 173 and 174:
Page 175 and 176:
Page 177 and 178:
Page 179 and 180:
Page 181 and 182:
Page 183 and 184:
Page 185 and 186:
Page 187 and 188:
Page 189 and 190:
Page 191 and 192:
Page 193 and 194:
Page 195 and 196:
Page 197 and 198:
Page 199 and 200:
Page 201 and 202:
Page 203 and 204:
Page 205 and 206:
Page 207 and 208:
Page 209 and 210:
Page 211 and 212:
Page 213 and 214:
Page 215 and 216:
Page 217 and 218:
Page 219 and 220:
Page 221 and 222:
Page 223 and 224:
Page 225 and 226:
Page 227 and 228:
Page 229 and 230:
Page 231 and 232:
Page 233 and 234:
show all

Reproduction in Domestic Animals - Facultad de Ciencias Veterinarias

You also want an ePaper? Increase the reach of your titles

Delete template?

Save as template?