Reproduction in Domestic Animals - Facultad de Ciencias Veterinarias
Reproduction in Domestic Animals - Facultad de Ciencias Veterinarias
Reproduction in Domestic Animals - Facultad de Ciencias Veterinarias
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16 t h International Congress on Animal <strong>Reproduction</strong><br />
Poster Abstracts 53<br />
As conclusion the BCSc could be associated positively with the ZICS<br />
and <strong>in</strong>versely with the time of ovarian activity postpartum.<br />
P076<br />
Evaluation of COX-2 and PGES gene expression <strong>in</strong> whole<br />
blood dur<strong>in</strong>g the peripartum of dairy cows<br />
Silva, E 1 ; Gaivão, M 1 ; Leitão, S 1 ; Costa, L 1 ; Mateus, L 1 *<br />
1Department of <strong>Reproduction</strong> and Obstetrics, C.I.I.S.A., Faculty of Veter<strong>in</strong>ary<br />
Medic<strong>in</strong>e, Portugal<br />
Dur<strong>in</strong>g the peripartum period dairy cows are immunosupressed and<br />
predisposed to <strong>de</strong>velop uter<strong>in</strong>e <strong>in</strong>fections. This has been associated to<br />
abnormal leukocyte function and to changes <strong>in</strong> neutrophil gene<br />
expression. High <strong>in</strong>tra-uter<strong>in</strong>e levels of prostagland<strong>in</strong> E 2 (PGE 2) were<br />
associated with <strong>de</strong>layed uter<strong>in</strong>e <strong>in</strong>volution and with the severity and<br />
persistence of endometritis. PGE 2 production is stimulated by LPS of<br />
gram-negative bacteria that contam<strong>in</strong>ate the puerperal uterus and act<br />
as a mediator of the <strong>in</strong>flammatory response. PGE 2 synthesis is<br />
accomplished by cyclooxygenase 2 (COX-2) and prostagland<strong>in</strong> E<br />
synthase (PGES) . The objectives of this study were to evaluate <strong>in</strong><br />
whole blood: i) the pattern of COX-2 and PGES gene expression<br />
dur<strong>in</strong>g the peripartum period and, ii) the gene expression response to<br />
LPS stimulation dur<strong>in</strong>g peripartum.<br />
Blood was collected from 17 dairy cows (9 had a normal puerperium<br />
and 8 <strong>de</strong>veloped uter<strong>in</strong>e <strong>in</strong>fection) at 1 and 2 weeks before parturition<br />
(wbp), at parturition and at 1 week after parturition. COX-2 and PGES<br />
gene expression, before and after whole blood stimulation with LPS,<br />
were quantified by Real Time PCR. Results were normalized with the<br />
housekeep<strong>in</strong>g gene ß2MG.<br />
COX-2 gene expression <strong>de</strong>creased until 6 hours before parturition and<br />
then significantly <strong>in</strong>creased (p