Reproduction in Domestic Animals - Facultad de Ciencias Veterinarias

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16 t h International Congress on Animal Reproduction 50 Poster Abstracts immunefluorescence (IFI) was used to detect CuZn-SOD presence from 133 CL slides. Statistical differences were determine by t-test (p
16 t h International Congress on Animal Reproduction Poster Abstracts 51 study investigated the relationships between these factors. A total of 84 normal beef heifers had their oestrous cycles synchronised using two injections of prostaglandin (PG), 11 days apart and were fitted with HeatWatch® devices. The time of initial standing event followed by successive mounting activity was taken as the onset of oestrus (D0). Heifers recorded in oestrus were inseminated 5 – 21hrs after onset using semen from a single ejaculate from a high fertility bull. Ovarian structures were ultrasonically examined with a 7.5-MHz probe starting 12 hours after onset of oestrus and repeated every 6 hours thereafter until ovulation (OV) occurred. Time of OV was determined from the time of the first scan on which the dominant follicle (DF) had disappeared minus 3 hrs. Ovaries were re-examined on day D7 to confirm OV and to measure luteal structures. Blood samples were collected at 12 hour intervals on D -1, D0 and again on D7 for IGF-I and P4 which were measured by IRMA and RIA, respectively. Embryo survival (EmSurv) was confirmed by ultrasonography at D30 and D100 post AI. The relationships between EmSurv and continuous variables were evaluated using logistic regression. EmSurv at D30 was 69% with two of the heifers suffering foetal loss between D30 and D100. OV occurred (Mean±S.D) 27.4 ± 5.9 h after the onset of heat. There was no relationship between the interval from onset of heat to OV or the interval from AI to OV and EmSurv. (P>0.05). There was evidence of a relationship between the size of the ovulatory follicle and EmSurv (Odds ratio=0.79; P=0.07). There was a positive relationship between concentration of P4 on Day 7 and EmSurv (Odds ratio=1.4; P0.05) between P4 on D7 and ovulatory follicle size, CL volume or concentrations of IGF-I on D-1, D0 or D7. Similarly there was no relationship (P>0.05) between IGF-I concentrations and EmSurv. We conclude that there was considerable variation in the timing of OV relative to the onset of oestrus. Time of AI relative to heat onset and or time of ovulation had no effect on embryo survival rate. There was a positive association between P4 on day 7 and embryo survival but not for IGF-I. Plasma P4 was not related to the size of the ovulatory follicle, CL volume or plasma IGF-I. Oocytes produced from large dominant follicles may have impaired embryo survival. P071 Prevalence of clinical and subclinical endometritis in dairy cows and the impact on reproductive performance Madoz, L 1 *, Ploentzke, J 2 , Albarracin, D 3 , Mejia, M 4 , Drillich, M 2 , Heuwieser, WS 2 , De La Sota, RL 1 1Catedra y Servicio de Reproduccion Animal, Facultad de Ciencias Veterinarias, Universidad Nacional de La Plata, Argentina; 2 Bovine Reproduction Clinic, Fac. Veterinary Sciences, Free University of Berlin, Germany; 3 Catedra de Patologia, Fac.de Cs. Veterinarias, Univ. Nac. de La Plata, Argentina; 4 Practica privada, Argentina The aim of this study was to evaluate the prevalence of clinical (CE) and subclinical (SE) endometritis and their impact on reproductive performance in dairy cows. Samples were collected from 211 Holstein cows in three farms in Argentina. Cows were examined for diagnosis of clinical endometritis (CE) between 21 and 62 days postpartum (dpp) at a monthly herd visit. At examination, cows were first inspected for presence of fresh and/or dry discharge on the vulva, perineum, or tail. Then the mucus content of the vagina was evaluated for color, proportion of pus to mucus, and odor; and a score was assigned as follows: clear mucus (0, [NOR]), predominantly clear mucus with flecks of pus (1, [CE1]), purulent mucus but not foulsmelling (2, [CE2]), or purulent or red-brown mucus and foul smelling (3, [CE3]). After clinical examination, if mucus content was NOR, cows were examined (EX1) for diagnosis of SE by endometrial cytology (n=165). Cows were reexamined 14±3 d later (EX2). Endometrial cytology samples were collected using a cytobrush modified for use in cattle. Cytology slides were prepared by rolling the CB on a clean glass microscope slide, air dried, fixed with ethylic alcohol and stored in a slide box. Slides were stained with a modified Wright-Giemsa stain and the degree of endometrial inflammation was assessed by counting a minimum of 200 cells at 400 x magnifications and expressed as the percent neutrophils (PPMN). The diagnosis criteria for SE was >18% PPMN in samples collected 21-33 dpp, >10% neutrophils in samples collected 34-47 dpp and >5% PPMN in samples collected 48-62 dpp. At 50 dpp, cows with normal mucus were detected in heat twice a day and AI. All AI cows were diagnosed pregnant by transrectal palpation at 35-65 d post AI. At examination, 78.2% (165/211) of cows were diagnosed NOR and 21.8% with CE (56.5% [26/46] CE1, 37.0% [17/46] CE2 and 6.5% [3/46] CE3). The cytobrush was done in 149 of 169 NOR cows. The prevalence of SE was 10.1% (15/149). At EX2, 87.5% (49/56) remained negative, 10.7% (6/56) changed from positive to negative and 1.8% (1/56) remained positive. Cows with SE needed more services per conception (2.77±0.45 vs. 1.96±0.18, p
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Reproduction in Domestic Animals - Facultad de Ciencias Veterinarias

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