Reproduction in Domestic Animals - Facultad de Ciencias Veterinarias
Reproduction in Domestic Animals - Facultad de Ciencias Veterinarias
Reproduction in Domestic Animals - Facultad de Ciencias Veterinarias
You also want an ePaper? Increase the reach of your titles
YUMPU automatically turns print PDFs into web optimized ePapers that Google loves.
16 t h International Congress on Animal <strong>Reproduction</strong><br />
Poster Abstracts 47<br />
P058<br />
The effect of periparturient propylene glycol<br />
adm<strong>in</strong>istration on metabolism and on reproductive<br />
performance <strong>in</strong> Holste<strong>in</strong>-Friesian cows<br />
Kerestes, M 1 *; Faigl, V 1 ; Győrffy, A 1 ; Márton , A 2 ; Langer, D 1 , Kulcsár, M 1 ;<br />
Gaál, T 1 ; Fébel, H 3 ; Húsvéth, F 2 ; Gabor, G 3 ; Bartha, T 1 ; Szenci, O 1 ;<br />
Huszenicza, G 1<br />
1Faculty of Veter<strong>in</strong>ary Science, Szent István University, Hungary; 2 Pannon<br />
University, Faculty of Agricultural Sciences, Keszthely, Hungary; 3 Institute of<br />
Animal Husbandry and Nutrition, Herceghalom, Hungary<br />
Negative energy balance <strong>in</strong> the early lactation is often accompanied<br />
by <strong>de</strong>creased <strong>in</strong>sul<strong>in</strong> secretion of the pancreatic β-cells and <strong>in</strong>sul<strong>in</strong><br />
resistance of the peripheral tissues. In dairy practice the propyleneglycol<br />
(1,2-propanediol, PGL) is often used for prevention of ketosis<br />
and fatty liver. We exam<strong>in</strong>ed the effect of periparturient PGL<br />
supplementation on metabolic profile, liver lipid content, and on<br />
reproductive performance <strong>in</strong> Holste<strong>in</strong> Friesian (HF) cows. The<br />
glucose-<strong>in</strong>duced <strong>in</strong>sul<strong>in</strong>-response of the β-cells and the <strong>in</strong>sul<strong>in</strong>sensitivity<br />
of peripheral tissues were also <strong>in</strong>vestigated.<br />
Fifty-one multiparous HF cows (previous lactation milk yield:<br />
8042±214 kg) <strong>in</strong> a large scale dairy herd were <strong>in</strong>volved <strong>in</strong> the study.<br />
The supplemented group (n=20) received PGL pow<strong>de</strong>r correspond<strong>in</strong>g<br />
to daily 5.05 MJ NE, from d14 before the expected calv<strong>in</strong>g date till<br />
d10 after calv<strong>in</strong>g, poured on the monodiet. The control group (n=30)<br />
did not received PGL. Blood samples were taken regularly for ßOHbutyrate<br />
(BHB), non esterified fatty acids (NEFA), glucose, <strong>in</strong>sul<strong>in</strong>,<br />
<strong>in</strong>sul<strong>in</strong> like growth hormone-I (IGF-I), thyrox<strong>in</strong>e (T 4 ) and 3,3',5,<br />
triiodtiron<strong>in</strong>e (T 3 ). On d7-10 after parturition <strong>in</strong> a subsequent of 16<br />
cows (Control=10; PGL=6) liver biopsy was taken to <strong>de</strong>term<strong>in</strong>e the<br />
hepatic total lipid content. After biopsy a simultaneous <strong>in</strong>travenous<br />
glucose and <strong>in</strong>sul<strong>in</strong> challenge test was performed. Dur<strong>in</strong>g the glucose<br />
tolerance test we exam<strong>in</strong>ed the glucose <strong>in</strong>duced <strong>in</strong>sul<strong>in</strong>-response area<br />
un<strong>de</strong>r the curve (AUC), the glucose-clearance rate, half time of<br />
glucose (T 1/2 ), and maximum <strong>in</strong>sul<strong>in</strong>-response time. From the <strong>in</strong>sul<strong>in</strong>tolerance<br />
test we measured the glucose response to the <strong>in</strong>sul<strong>in</strong>.<br />
Resumption of cyclicity was monitored by milk progesterone profiles<br />
from samples collected 3 times per week. After pre-synchronisation of<br />
the ovarian activity with two PGF2a <strong>in</strong>jections at 12 days <strong>in</strong>terval, a<br />
GPG protocol and fixed time AI was performed.<br />
The PGL supplementation <strong>in</strong>creased the <strong>in</strong>sul<strong>in</strong> concentrations and<br />
<strong>de</strong>creased the BHB levels <strong>in</strong> the last days of pregnancy. The treatment<br />
had no effect on blood glucose, thyroid hormone and IGF-I levels.<br />
The parameters measured dur<strong>in</strong>g the glucose- and <strong>in</strong>sul<strong>in</strong>-tolerance<br />
test were not changed. In cows with subcl<strong>in</strong>ical form ketosis the<br />
glucose-<strong>in</strong>duced <strong>in</strong>sul<strong>in</strong>-response and the glucose T 1/2 were notably<br />
lower. PGL adm<strong>in</strong>istration had no effect on the time of the first pp<br />
ovulation (34.1±18vs. 34±16d) and on pregnancy rate (38% vs. 32%)<br />
of the animals. The periparturient PGL supplementation somewhat<br />
affected the metabolic status of the cows, but its effects rema<strong>in</strong>ed<br />
below our expectations.<br />
P059<br />
Corpus luteum function and morphology <strong>in</strong> relation to<br />
nutrition <strong>in</strong> dairy cows<br />
Knijn, HM 1 *, Uijttewaal, MJ 1 , Dieleman, SJ 1 , van <strong>de</strong>n Hurk, R 1 , Zaaijer, D 6 ,<br />
Vos, PLAM 1<br />
1Farm Animal Health, Faculty of Veter<strong>in</strong>ary Medic<strong>in</strong>e, Utrecht University, The<br />
Netherlands; 6 DAP Future Fertility Systems, The Netherlands<br />
Anoestrus is an important problem <strong>in</strong> high yield<strong>in</strong>g dairy cows, which<br />
can have different causes, for example, a prolonged luteal phase.<br />
Recently <strong>in</strong> veter<strong>in</strong>ary practice, abnormal, large, spongy corpora lutea<br />
(CLs) were diagnosed <strong>in</strong> non-cyclic dairy cows post partum. These<br />
cows received a typical diet with low levels of sugar and rumen<br />
fermentable starch, and a relatively high fraction of rumen<br />
un<strong>de</strong>gradable starch, <strong>in</strong> comb<strong>in</strong>ation with high levels of prote<strong>in</strong>. The<br />
aim of the present study was to create a mo<strong>de</strong>l to <strong>in</strong>duce spongy CLs<br />
by feed<strong>in</strong>g this typical diet to dairy cows, and to <strong>in</strong>vestigate its effect<br />
on CL morphology and function <strong>in</strong> comparison to control diet.<br />
Twenty-four animals were selected based on parity (1-3), lactation<br />
state (3-6 months <strong>in</strong> lactation) and normal cyclicity. All animals were<br />
synchronized and at the end of the follow<strong>in</strong>g cycle they were allocated<br />
<strong>in</strong>to three diet groups; rape- (high rumen fermentable prote<strong>in</strong> > 18%<br />
ma<strong>in</strong>ly from rape, low fermentable energy), soya- (high rumen<br />
fermentable prote<strong>in</strong> >18% ma<strong>in</strong>ly from soya, low fermentable energy)<br />
and control group. Blood samples for progesterone analyses were<br />
collected three times a week. The animals were ovariectomized at day<br />
12 (day 0 is estrus) of the third cycle <strong>in</strong> which they were fed the 3<br />
different diets. The obta<strong>in</strong>ed CLs were analysed histologically with<br />
regards to surface and size of luteal cells us<strong>in</strong>g as marker 3-β-HSD<br />
expression (a pivotal enzyme <strong>in</strong> the synthesis of progesterone).<br />
Furthermore, the mRNA expression for IGF-1 (which stimulates the<br />
steroidogenesis), and lept<strong>in</strong> (which is a elusive factor l<strong>in</strong>k<strong>in</strong>g<br />
reproduction and metabolic status) and their receptors was quantified<br />
by Q-PCR technique.<br />
Dur<strong>in</strong>g this trial, no spongy CLs have <strong>de</strong>veloped but a large variety of<br />
both macro- and microscopic appearance of the CLs has been<br />
observed. Remarkably, this was the first time that adipose tissue was<br />
<strong>de</strong>scribed <strong>in</strong> the CL, which was frequently observed near the centre or<br />
cavity of the CL. In this respect, a significant difference (p