Reproduction in Domestic Animals - Facultad de Ciencias Veterinarias

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16 t h International Congress on Animal Reproduction 46 Poster Abstracts The study was carried out on 44 multiparous lactating Holestein cows (FCM = 7759.1 kg) in a commercial dairy herd. Scanning of the ovaries was performed by transrectal ultrasound (5 MHz, Ami, Canada) and blood samples for P 4 analysis were collected twice weekly commencing from day 7 to day 66 after calving. Serum P 4 concentration was measured by a commercial validated radioimmunoassay kit (Immunotech kit, France). Cows with normal or abnormal postpartum ovarian activities were defined based on the characteristics of their serum P 4 profiles (Shrestha et al., 2004. Theriogenology 61:637-49). Of 44 cows studied, 22 (50 %) had normal ovarian activity (ovulation occurring ≤45 days after calving, followed by regular ovarian cycles); while, 17 (38.6 %) and 5 (11.4 %) had delayed first ovulation (DOV) and prolonged luteal phase (PLP), respectively. Data were statistically analysed using Anova. Logistic regression analysis indicated a significant interaction between P 4 concentration on day 24 after calving and peak milk yield (kg/day, 75-d postpartum). With the limited number of PLP observed cows in the present study, statistical analyses showed that the likelihood of the occurrence of PLP increased by 1.1 for each 1 kg increase in peak milk yield of cows with P 4 concentration ≥ 1 ng/ml on day 24 after calving (P=0.01, 95% CI=1.01-1.15). First postpartum P 4 rise was observed significantly earlier in cows with PLP compared to that of the cows with normal ovarian activity (23.2 ± 4.0 vs 32.2 ± 8.38; P
16 t h International Congress on Animal Reproduction Poster Abstracts 47 P058 The effect of periparturient propylene glycol administration on metabolism and on reproductive performance in Holstein-Friesian cows Kerestes, M 1 *; Faigl, V 1 ; Győrffy, A 1 ; Márton , A 2 ; Langer, D 1 , Kulcsár, M 1 ; Gaál, T 1 ; Fébel, H 3 ; Húsvéth, F 2 ; Gabor, G 3 ; Bartha, T 1 ; Szenci, O 1 ; Huszenicza, G 1 1Faculty of Veterinary Science, Szent István University, Hungary; 2 Pannon University, Faculty of Agricultural Sciences, Keszthely, Hungary; 3 Institute of Animal Husbandry and Nutrition, Herceghalom, Hungary Negative energy balance in the early lactation is often accompanied by decreased insulin secretion of the pancreatic β-cells and insulin resistance of the peripheral tissues. In dairy practice the propyleneglycol (1,2-propanediol, PGL) is often used for prevention of ketosis and fatty liver. We examined the effect of periparturient PGL supplementation on metabolic profile, liver lipid content, and on reproductive performance in Holstein Friesian (HF) cows. The glucose-induced insulin-response of the β-cells and the insulinsensitivity of peripheral tissues were also investigated. Fifty-one multiparous HF cows (previous lactation milk yield: 8042±214 kg) in a large scale dairy herd were involved in the study. The supplemented group (n=20) received PGL powder corresponding to daily 5.05 MJ NE, from d14 before the expected calving date till d10 after calving, poured on the monodiet. The control group (n=30) did not received PGL. Blood samples were taken regularly for ßOHbutyrate (BHB), non esterified fatty acids (NEFA), glucose, insulin, insulin like growth hormone-I (IGF-I), thyroxine (T 4 ) and 3,3',5, triiodtironine (T 3 ). On d7-10 after parturition in a subsequent of 16 cows (Control=10; PGL=6) liver biopsy was taken to determine the hepatic total lipid content. After biopsy a simultaneous intravenous glucose and insulin challenge test was performed. During the glucose tolerance test we examined the glucose induced insulin-response area under the curve (AUC), the glucose-clearance rate, half time of glucose (T 1/2 ), and maximum insulin-response time. From the insulintolerance test we measured the glucose response to the insulin. Resumption of cyclicity was monitored by milk progesterone profiles from samples collected 3 times per week. After pre-synchronisation of the ovarian activity with two PGF2a injections at 12 days interval, a GPG protocol and fixed time AI was performed. The PGL supplementation increased the insulin concentrations and decreased the BHB levels in the last days of pregnancy. The treatment had no effect on blood glucose, thyroid hormone and IGF-I levels. The parameters measured during the glucose- and insulin-tolerance test were not changed. In cows with subclinical form ketosis the glucose-induced insulin-response and the glucose T 1/2 were notably lower. PGL administration had no effect on the time of the first pp ovulation (34.1±18vs. 34±16d) and on pregnancy rate (38% vs. 32%) of the animals. The periparturient PGL supplementation somewhat affected the metabolic status of the cows, but its effects remained below our expectations. P059 Corpus luteum function and morphology in relation to nutrition in dairy cows Knijn, HM 1 *, Uijttewaal, MJ 1 , Dieleman, SJ 1 , van den Hurk, R 1 , Zaaijer, D 6 , Vos, PLAM 1 1Farm Animal Health, Faculty of Veterinary Medicine, Utrecht University, The Netherlands; 6 DAP Future Fertility Systems, The Netherlands Anoestrus is an important problem in high yielding dairy cows, which can have different causes, for example, a prolonged luteal phase. Recently in veterinary practice, abnormal, large, spongy corpora lutea (CLs) were diagnosed in non-cyclic dairy cows post partum. These cows received a typical diet with low levels of sugar and rumen fermentable starch, and a relatively high fraction of rumen undegradable starch, in combination with high levels of protein. The aim of the present study was to create a model to induce spongy CLs by feeding this typical diet to dairy cows, and to investigate its effect on CL morphology and function in comparison to control diet. Twenty-four animals were selected based on parity (1-3), lactation state (3-6 months in lactation) and normal cyclicity. All animals were synchronized and at the end of the following cycle they were allocated into three diet groups; rape- (high rumen fermentable protein > 18% mainly from rape, low fermentable energy), soya- (high rumen fermentable protein >18% mainly from soya, low fermentable energy) and control group. Blood samples for progesterone analyses were collected three times a week. The animals were ovariectomized at day 12 (day 0 is estrus) of the third cycle in which they were fed the 3 different diets. The obtained CLs were analysed histologically with regards to surface and size of luteal cells using as marker 3-β-HSD expression (a pivotal enzyme in the synthesis of progesterone). Furthermore, the mRNA expression for IGF-1 (which stimulates the steroidogenesis), and leptin (which is a elusive factor linking reproduction and metabolic status) and their receptors was quantified by Q-PCR technique. During this trial, no spongy CLs have developed but a large variety of both macro- and microscopic appearance of the CLs has been observed. Remarkably, this was the first time that adipose tissue was described in the CL, which was frequently observed near the centre or cavity of the CL. In this respect, a significant difference (p
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Reproduction in Domestic Animals - Facultad de Ciencias Veterinarias

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