Reproduction in Domestic Animals - Facultad de Ciencias Veterinarias
Reproduction in Domestic Animals - Facultad de Ciencias Veterinarias
Reproduction in Domestic Animals - Facultad de Ciencias Veterinarias
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16 t h International Congress on Animal <strong>Reproduction</strong><br />
Poster Abstracts 43<br />
and both ITGAD and ITGB7 were only expressed at the hatched<br />
blastocyst stage.<br />
This study <strong>de</strong>monstrates the variability <strong>in</strong> <strong>in</strong>tegr<strong>in</strong> expression dur<strong>in</strong>g<br />
preimplantation embryo <strong>de</strong>velopment and pushes ITGα3β1, ITGα5β1<br />
and ITGαVβ3 forward as candidate receptors for the embryo specific<br />
FN1 splice variant. Further research is necessary to reveal the<br />
subcellular distribution and organization of the <strong>in</strong>tegr<strong>in</strong> prote<strong>in</strong>s as<br />
well as downstream adhesion and signal transduction pathways.<br />
P046<br />
Reproductive management strategies to improve<br />
pregnancy rate follow<strong>in</strong>g Ovsynch/TAI protocol <strong>in</strong> dairy<br />
cows and heifers<br />
Gordon, M 1 *, D<strong>in</strong>n, N 2<br />
1Animal Science, University of British Columbia, Canada; 2 Ubc Dairy<br />
Education & Research Centre, Canada<br />
Physiological and environmental stresses of high milk production and<br />
<strong>in</strong>tensive management systems have affected the onset of postpartum<br />
ovarian activity, expression of estrus, embryonic <strong>de</strong>velopment, and<br />
pregnancy <strong>in</strong> lactat<strong>in</strong>g dairy cows. Though prostagland<strong>in</strong> (PGF2α) is<br />
still the most wi<strong>de</strong>ly used drug for the <strong>in</strong>duction and synchronization<br />
of estrus, the Ovsynch/TAI protocol, which strategically uses GnRH<br />
and PGF2α to synchronize ovulation offers potential freedom to dairy<br />
farmers from the time consum<strong>in</strong>g chore of estrus <strong>de</strong>tection. The<br />
ovulation rate and pregnancy rate (PR) with Ovsynch/TAI protocol <strong>in</strong><br />
postpartum cows are around 80% and 30 to 40%, respectively and<br />
lower <strong>in</strong> heifers. However, pre-synchronization before Ovsynch/TAI<br />
protocol or the use of GnRH five to seven days after breed<strong>in</strong>g has the<br />
potential to improve PR and this was tested <strong>in</strong> the current study.<br />
Multiparous and primiparous lactat<strong>in</strong>g cows (n=225) and nulliparous<br />
heifers (n = 87) were used. <strong>Animals</strong> were randomly assigned to one of<br />
three treatments for timed first service breed<strong>in</strong>g (TAI) at about 75<br />
days <strong>in</strong> milk (DIM) for cows and 15 months of age for heifers: A)<br />
Control - Ovsynch protocol (GnRH <strong>in</strong>jection given 7 d before and<br />
another 48 h after one PGF2α <strong>in</strong>jection); B) Presynch + Ovsynch (two<br />
<strong>in</strong>jections of PGF2α 14 d apart followed by Ovsynch 14 d later); C)<br />
Ovsynch + post-AI GnRH (a GnRH <strong>in</strong>jection given 6 d after TAI<br />
follow<strong>in</strong>g Ovsynch). A total of 10 blood samples (from heifers) or<br />
milk samples (from cows) were taken from each animal (on days -38,<br />
-31, -24, -10, -3, 0 (TAI), 7, 14, 21, 28) for progesterone (P4)<br />
analysis. Pregnancy rate for heifers were 65%, 59%, and 59% and for<br />
lactat<strong>in</strong>g cows were 44%, 48%, and 46% for treatments A, B, and C,<br />
respectively, reveal<strong>in</strong>g no treatment effect on PR (p>0.05). However,<br />
there was an <strong>in</strong>teraction of DIM and BCS at TAI with PR (p=0.008<br />
and p=0.20, respectively). The trend for cows <strong>in</strong> less BCS and less<br />
DIM to have lower PR was seen most <strong>in</strong> the Ovsynch group.<br />
Moreover, pregnant animals <strong>in</strong> group B and C had higher P4 levels on<br />
day 21 and day 28 than did pregnant animals from control group<br />
(p=0.15), which could be <strong>in</strong>dicative of better function<strong>in</strong>g CL’s and<br />
thus enhanc<strong>in</strong>g PR. These results show that the Ovsynch protocol can<br />
be used to achieve acceptable PR rates <strong>in</strong> heifers us<strong>in</strong>g a synchronized<br />
TAI protocol. Although differences <strong>in</strong> pregnancy were not significant<br />
among treatments, PR and P4 results from this study show that<br />
presynchronization and post <strong>in</strong>sem<strong>in</strong>ation GnRH tend to benefit cows<br />
which are <strong>in</strong> more negative energy balance.<br />
P047<br />
The effect of early or late breed<strong>in</strong>g on milk production <strong>in</strong><br />
lactat<strong>in</strong>g dairy cows<br />
Gumen, A 1 *, Kesk<strong>in</strong>, A 1 , Yılmazbas, G 1 , Celik, Y 2 , Burucu, Y 2<br />
1University of Uludag, Faculty of Veter<strong>in</strong>ary Medic<strong>in</strong>e, Department of<br />
Obstetrics and Gynecology, Bursa, Turkey; 2 Tarfaş A. Ş., Karacabey, Bursa,<br />
Turkey<br />
Milk production and reproduction are two important factors with<br />
respect to profitability of dairy farms and much attention has been<br />
given to fertility parameters and their association with milk<br />
production. Previous studies was reported that pregnancy adversely<br />
affect milk production. Delay<strong>in</strong>g of first breed<strong>in</strong>g <strong>in</strong> high produc<strong>in</strong>g<br />
dairy cows may <strong>in</strong>crease overall milk yield. The objective of this<br />
study was to compare effect of early or late breed<strong>in</strong>g on milk<br />
production <strong>in</strong> lactat<strong>in</strong>g dairy cows. Dairy cows (n=52) were divi<strong>de</strong>d<br />
<strong>in</strong>to two groups. In group 1 (early bred), cows (n=22) were<br />
<strong>in</strong>sem<strong>in</strong>ated between 45 to 75 postpartum and group 2 (n=30; late<br />
bred) were <strong>in</strong>sem<strong>in</strong>ated between 76 to 130 postpartum. All cows were<br />
<strong>in</strong> second lactation. Cows were fed twice daily with a high-energy<br />
lactat<strong>in</strong>g dairy cow ration fed as a TMR follow<strong>in</strong>g NRC<br />
recommendations. Estrus <strong>de</strong>tection was recor<strong>de</strong>d with pedometer and<br />
visual observation. Milk yield was recor<strong>de</strong>d every 5 days after calv<strong>in</strong>g<br />
first 45 days then every 15 days until 270 days. Breed<strong>in</strong>g of cows<br />
were <strong>in</strong>itiated after voluntary wait<strong>in</strong>g period (45 days <strong>in</strong> milk) with<br />
artificial <strong>in</strong>sem<strong>in</strong>ation. Cows were <strong>in</strong>sem<strong>in</strong>ated only once and became<br />
pregnant follow<strong>in</strong>g their first postpartum estrus <strong>in</strong>clu<strong>de</strong>d <strong>in</strong> this study.<br />
Average days <strong>in</strong> milk <strong>in</strong> early and late bred cows at the time of<br />
artificial <strong>in</strong>sem<strong>in</strong>ation were 65.2 and 102.6 days, respectively.<br />
Average milk yield first 270 days was 37.8 and 39.0 kg for early and<br />
late bred cows. Milk yield was not different between early and late<br />
bred cows from calv<strong>in</strong>g to 270 days after calv<strong>in</strong>g. There was no<br />
relationship between first 3 months milk yield and postpartum days of<br />
<strong>in</strong>sem<strong>in</strong>ation. Also, there was no relationship between overall milk<br />
yied (calv<strong>in</strong>g to 270 days) and postpartum days of <strong>in</strong>sem<strong>in</strong>ation.<br />
Thus, milk yield was not affected by early or late breed<strong>in</strong>g of cows<br />
but further trials are nee<strong>de</strong>d to evaluate the repeatability of this<br />
response.<br />
P048<br />
Effect of ketoprofen adm<strong>in</strong>istration 15 and 16 days after<br />
AI on conception rates <strong>in</strong> lactat<strong>in</strong>g Holste<strong>in</strong> cows<br />
Guzeloglu, A 1 *, Er<strong>de</strong>m, H 2 , C<strong>in</strong>ar, M 3 , Kilic, K 4 , Talmac, M 4 , Gorgundur, A 4 ,<br />
Gumen, A 5<br />
1Obstetrics And Gyneacology, Selcuk University Faculty of Veter<strong>in</strong>ary<br />
Medic<strong>in</strong>e, Turkey; 2 Selcuk University Faculty of Veter<strong>in</strong>ary Medic<strong>in</strong>e, Turkey;<br />
3Nig<strong>de</strong> University, Bor Vocational School, Turkey; 4 Kocas Tigem Agricultural<br />
Station Aksaray, Turkey; 5 Uludag University Faculty of Veter<strong>in</strong>ary Medic<strong>in</strong>e,<br />
Turkey<br />
It has been reported that timely adm<strong>in</strong>istration of a non-steroid anti<strong>in</strong>flammatory<br />
drug (NSAID), flunix<strong>in</strong> meglum<strong>in</strong>e, around the time of<br />
luteolysis (day 15 and day 16) <strong>in</strong>creased pregnancy rates <strong>in</strong> heifers<br />
(Guzeloglu et al., 2007) and lactat<strong>in</strong>g cows (Pfeifer et al., 2007). The<br />
objective of this study was to <strong>de</strong>term<strong>in</strong>e if two <strong>in</strong>jections of<br />
ketoprofen would <strong>de</strong>monstrate the same effect as flunix<strong>in</strong> meglum<strong>in</strong>e.<br />
Ketoprofen has no milk residue <strong>in</strong> contrast to flunix<strong>in</strong> meglum<strong>in</strong>e.<br />
Two experiments were done <strong>in</strong> lactat<strong>in</strong>g Holste<strong>in</strong> dairy cows (milk<br />
yield average 25 kg/day, 3 to 10 years old, average DIM 125 days). In<br />
experiment 1, 86 cows were <strong>in</strong>sem<strong>in</strong>ated at <strong>de</strong>tected heat and<br />
randomly assigned to receive treatment (n=45; ketoprofen, i.m., 3<br />
mg/kg BW on day 15 and 16 after <strong>in</strong>sem<strong>in</strong>ation; 24 hours apart) or<br />
control (n=41). In experiment 2, 84 cows were <strong>in</strong>sem<strong>in</strong>ated at fixed<br />
time (TAI) follow<strong>in</strong>g ovsynch program and randomly assigned to<br />
treatment (n=43) and control (n=41) groups as <strong>de</strong>scribed for<br />
experiment 1. Pregnancy diagnosis was performed by ultrasonography<br />
30 days after TAI. Conception rates did not differ between treatment<br />
and control groups (In experiment 1, 47% (21/45) for treatment and<br />
49% (20/41) control; <strong>in</strong> experiment 2, 30% (13/43) for treatment and<br />
27% (11/41) control). In both experiments, no significant effects of<br />
sire, days <strong>in</strong> milk, milk yield and parity were <strong>de</strong>tected. In conclusion,<br />
ketoprofen did not have beneficial effect on conception rates <strong>in</strong><br />
lactat<strong>in</strong>g cows as flunix<strong>in</strong> meglum<strong>in</strong>e. Moreover, data suggests that<br />
ketoprofen can be used <strong>in</strong> early pregnancy for treatment of possible<br />
<strong>in</strong>flammatory conditions without hav<strong>in</strong>g <strong>de</strong>trimental effects on<br />
pregnancy.<br />
P049<br />
Relationship between uter<strong>in</strong>e <strong>in</strong>volution and pregnancy<br />
rate <strong>in</strong> dairy cows<br />
Hajurka, J<br />
Equ<strong>in</strong>e cl<strong>in</strong>ic, University of Vter<strong>in</strong>ary Medic<strong>in</strong>e, Slovakia<br />
The objectives of this study were to assess uter<strong>in</strong>e <strong>in</strong>volution and<br />
pregnancy rate <strong>in</strong> dairy cows un<strong>de</strong>r field condition. <strong>Animals</strong> were