Reproduction in Domestic Animals - Facultad de Ciencias Veterinarias

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16 t h International Congress on Animal Reproduction 42 Poster Abstracts increase in days to CLA by selection for high yields can be reduced by including selection for non-return rate without reduction in milk yield, as evidenced by equal production levels in HMP- and HGMcows. P043 Benchmarking in Reproductive Control Programs of tropical dual purpose crossbred herds González-Stagnaro, C Zootecnia, Universidad Del Zulia, Facultad De Agronomía, Instituto De Investigaciones Agronómicas, Venezuela Medicine of Production, TQM and HACCP has proved that reengineering livestock process, offer a new way to organize farm works, applying updated technologies to improve production and profit. In addition, has demonstrated that farmers are different, open for research, but reluctant to make changes and adopt new technologies; they only will take decisions, when the successfully farmer shows their technical changes and economical benefits. Benchmarking methodology adopted management practices from more profitable farms, supported by the records evaluation, computerized information and statistical process. This ¨strategic emulation¨ of success practices by the less efficient farms, will increase productive, reproductive and economic performance. The first objective of this benchmarking work was the study of the frequency of application of 33 technologies and biotechonogies in 32 dual purpose crossbred herds Bos taurus x Bos indicus: 16 improved (IM) and 16 traditional (TM) managed system, located in Zulia state, Venezuela (10ºNL, 32-36ºC, 1100mm). Differences between systems were analyzed by “t” test. There were differences (P
16 t h International Congress on Animal Reproduction Poster Abstracts 43 and both ITGAD and ITGB7 were only expressed at the hatched blastocyst stage. This study demonstrates the variability in integrin expression during preimplantation embryo development and pushes ITGα3β1, ITGα5β1 and ITGαVβ3 forward as candidate receptors for the embryo specific FN1 splice variant. Further research is necessary to reveal the subcellular distribution and organization of the integrin proteins as well as downstream adhesion and signal transduction pathways. P046 Reproductive management strategies to improve pregnancy rate following Ovsynch/TAI protocol in dairy cows and heifers Gordon, M 1 *, Dinn, N 2 1Animal Science, University of British Columbia, Canada; 2 Ubc Dairy Education & Research Centre, Canada Physiological and environmental stresses of high milk production and intensive management systems have affected the onset of postpartum ovarian activity, expression of estrus, embryonic development, and pregnancy in lactating dairy cows. Though prostaglandin (PGF2α) is still the most widely used drug for the induction and synchronization of estrus, the Ovsynch/TAI protocol, which strategically uses GnRH and PGF2α to synchronize ovulation offers potential freedom to dairy farmers from the time consuming chore of estrus detection. The ovulation rate and pregnancy rate (PR) with Ovsynch/TAI protocol in postpartum cows are around 80% and 30 to 40%, respectively and lower in heifers. However, pre-synchronization before Ovsynch/TAI protocol or the use of GnRH five to seven days after breeding has the potential to improve PR and this was tested in the current study. Multiparous and primiparous lactating cows (n=225) and nulliparous heifers (n = 87) were used. Animals were randomly assigned to one of three treatments for timed first service breeding (TAI) at about 75 days in milk (DIM) for cows and 15 months of age for heifers: A) Control - Ovsynch protocol (GnRH injection given 7 d before and another 48 h after one PGF2α injection); B) Presynch + Ovsynch (two injections of PGF2α 14 d apart followed by Ovsynch 14 d later); C) Ovsynch + post-AI GnRH (a GnRH injection given 6 d after TAI following Ovsynch). A total of 10 blood samples (from heifers) or milk samples (from cows) were taken from each animal (on days -38, -31, -24, -10, -3, 0 (TAI), 7, 14, 21, 28) for progesterone (P4) analysis. Pregnancy rate for heifers were 65%, 59%, and 59% and for lactating cows were 44%, 48%, and 46% for treatments A, B, and C, respectively, revealing no treatment effect on PR (p>0.05). However, there was an interaction of DIM and BCS at TAI with PR (p=0.008 and p=0.20, respectively). The trend for cows in less BCS and less DIM to have lower PR was seen most in the Ovsynch group. Moreover, pregnant animals in group B and C had higher P4 levels on day 21 and day 28 than did pregnant animals from control group (p=0.15), which could be indicative of better functioning CL’s and thus enhancing PR. These results show that the Ovsynch protocol can be used to achieve acceptable PR rates in heifers using a synchronized TAI protocol. Although differences in pregnancy were not significant among treatments, PR and P4 results from this study show that presynchronization and post insemination GnRH tend to benefit cows which are in more negative energy balance. P047 The effect of early or late breeding on milk production in lactating dairy cows Gumen, A 1 *, Keskin, A 1 , Yılmazbas, G 1 , Celik, Y 2 , Burucu, Y 2 1University of Uludag, Faculty of Veterinary Medicine, Department of Obstetrics and Gynecology, Bursa, Turkey; 2 Tarfaş A. Ş., Karacabey, Bursa, Turkey Milk production and reproduction are two important factors with respect to profitability of dairy farms and much attention has been given to fertility parameters and their association with milk production. Previous studies was reported that pregnancy adversely affect milk production. Delaying of first breeding in high producing dairy cows may increase overall milk yield. The objective of this study was to compare effect of early or late breeding on milk production in lactating dairy cows. Dairy cows (n=52) were divided into two groups. In group 1 (early bred), cows (n=22) were inseminated between 45 to 75 postpartum and group 2 (n=30; late bred) were inseminated between 76 to 130 postpartum. All cows were in second lactation. Cows were fed twice daily with a high-energy lactating dairy cow ration fed as a TMR following NRC recommendations. Estrus detection was recorded with pedometer and visual observation. Milk yield was recorded every 5 days after calving first 45 days then every 15 days until 270 days. Breeding of cows were initiated after voluntary waiting period (45 days in milk) with artificial insemination. Cows were inseminated only once and became pregnant following their first postpartum estrus included in this study. Average days in milk in early and late bred cows at the time of artificial insemination were 65.2 and 102.6 days, respectively. Average milk yield first 270 days was 37.8 and 39.0 kg for early and late bred cows. Milk yield was not different between early and late bred cows from calving to 270 days after calving. There was no relationship between first 3 months milk yield and postpartum days of insemination. Also, there was no relationship between overall milk yied (calving to 270 days) and postpartum days of insemination. Thus, milk yield was not affected by early or late breeding of cows but further trials are needed to evaluate the repeatability of this response. P048 Effect of ketoprofen administration 15 and 16 days after AI on conception rates in lactating Holstein cows Guzeloglu, A 1 *, Erdem, H 2 , Cinar, M 3 , Kilic, K 4 , Talmac, M 4 , Gorgundur, A 4 , Gumen, A 5 1Obstetrics And Gyneacology, Selcuk University Faculty of Veterinary Medicine, Turkey; 2 Selcuk University Faculty of Veterinary Medicine, Turkey; 3Nigde University, Bor Vocational School, Turkey; 4 Kocas Tigem Agricultural Station Aksaray, Turkey; 5 Uludag University Faculty of Veterinary Medicine, Turkey It has been reported that timely administration of a non-steroid antiinflammatory drug (NSAID), flunixin meglumine, around the time of luteolysis (day 15 and day 16) increased pregnancy rates in heifers (Guzeloglu et al., 2007) and lactating cows (Pfeifer et al., 2007). The objective of this study was to determine if two injections of ketoprofen would demonstrate the same effect as flunixin meglumine. Ketoprofen has no milk residue in contrast to flunixin meglumine. Two experiments were done in lactating Holstein dairy cows (milk yield average 25 kg/day, 3 to 10 years old, average DIM 125 days). In experiment 1, 86 cows were inseminated at detected heat and randomly assigned to receive treatment (n=45; ketoprofen, i.m., 3 mg/kg BW on day 15 and 16 after insemination; 24 hours apart) or control (n=41). In experiment 2, 84 cows were inseminated at fixed time (TAI) following ovsynch program and randomly assigned to treatment (n=43) and control (n=41) groups as described for experiment 1. Pregnancy diagnosis was performed by ultrasonography 30 days after TAI. Conception rates did not differ between treatment and control groups (In experiment 1, 47% (21/45) for treatment and 49% (20/41) control; in experiment 2, 30% (13/43) for treatment and 27% (11/41) control). In both experiments, no significant effects of sire, days in milk, milk yield and parity were detected. In conclusion, ketoprofen did not have beneficial effect on conception rates in lactating cows as flunixin meglumine. Moreover, data suggests that ketoprofen can be used in early pregnancy for treatment of possible inflammatory conditions without having detrimental effects on pregnancy. P049 Relationship between uterine involution and pregnancy rate in dairy cows Hajurka, J Equine clinic, University of Vterinary Medicine, Slovakia The objectives of this study were to assess uterine involution and pregnancy rate in dairy cows under field condition. Animals were
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Reproduction in Domestic Animals - Facultad de Ciencias Veterinarias

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