Reproduction in Domestic Animals - Facultad de Ciencias Veterinarias

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16 t h International Congress on Animal Reproduction 40 Poster Abstracts P037 Treatment of Uterine Infections in Non Cycling Cows with Cloprostenol Fernandes, C 1-2 *; Figueiredo, A 1-2 ; Alves, B 2 ; Oliveira, E 2 ; Viana, J 4 , Gioso, M 1; Oba, E 4 1Faculty of Veterinary Medicine, University of Alfenas, Brazil; 2 Biotran LTDA, Brazil; 3 Embrapa-Gado de Leite, Brazil; 4 FMVZ-Unesp – Botucatu, Brazil Recent findings on the mechanisms of uterine defense show the eicosanoids as the main substances that modulate this activity. This observation open an excellent chance for the treatment of uterine infections, once the PGF2α and its analogous have activity on the production of these substances in the uterus. Moreover, they would provoke reduction in the progesterone (P4). P4 produces substances that inhibit the uterine defense mechanisms and partially block the production of eicosanoids. The application of PGF analogous was capable of stimulating the PGF2 and other related substances production in the uterus, as Leucotriene B4, which activate some leucocitary functions, associated to the neutrophile, the most important cell in the uterus defense mechanism. The aims of this study were to evaluate and compare the efficiency of protocols of Cloprostenol administration for the treatment of clinical endometritis in dairy cows (Holstein) without luteal ovarian activity. Cows of six herds were used, all presenting clinical endometritis, associated with a involuted uterus (30-118 days post-partum) and absence of corpus luteum. The diagnosis and classification of the endometritis was based on abnormal uterine discharge on vaginoscopic examination. In accordance with the infection degree (mucopurulent or purulent) the animals had been randomized in four groups, and received IM treatments: T1 (n=22): 2ml of saline solution; T2 (n=41): only one dose of 0.530mg of Cloprostenol (2ml Ciosin Schering Plough- Brazil); T3 (n=43): 2 doses of 0.530mg of Cloprostenol 24 hours apart and T4 (n=40): 2 doses 0.530mg of Cloprostenol with interval of 48 hours. The cows were evaluated by vaginoscopy 20 to 30 days later. The efficiency between the treatments was compared by the χ2 test. There was no herd effect in treatment results. The efficiency was 18.18a; 39.02b, 71.42c and 47.50%b for groups T1, T2, T3 and T4, respectively. The treatment with better efficiency was T3, (P5 mm), and number of follicles ≥4 mm on D7 were analyzed by logistic regression using Glimmix procedure. Daily size of follicles was analyzed by MIXED procedure. Surprisingly, treatment with EB did not delay emergence of the follicular wave (1.40±0.24 vs. 1.40±0.24 days, P=1.00) or alter largest follicle size at any day post-treatment. In addition, there was no difference between control and EB-treated cows in growth rate of the largest follicle from D1 to D7 (1.50±0.05 vs. 1.21±0.16 mm/d; P=0.34) or D4 to D7 (1.20±0.17 vs. 1.40±0.16 mm/d; P=0.53); but, EB decreased growth of the largest follicle from D1 to D4 (1.25±0.15 vs. 0.83±0.13 mm/d; P=0.03). Unexpectedly, EB treatment increased number of follicles ≥4mm on Days 5, 6, or 7 (D7; Control=7.2±0.8 vs. EB=21.2±5.3 follicles; P=0.03). Thus, a dose of EB that normally delays follicular wave emergence by ~4d was not effective in delaying emergence or growth of the dominant follicle after follicular aspiration perhaps due to absence of follicular products such as inhibin. However, EB treatment following follicular aspiration produced a dramatic increase in the numbers of small follicles even in the presence of a dominant follicle. P039 Ovarian Cysts Treatment with Fertirelin Associated or not to Cloprostenol Fernandes, C 1-2 ; Figueiredo, A 1-2 *; Alves, B 2 ; Oliveira, E 2 ; Viana, J 4 , Gioso, M 1 ; Oba, E 4 1Faculty of Veterinary Medicine, University of Alfenas, Brazil; 2 Biotran LTDA, Brazil; 3 Embrapa-Gado de Leite, Brazil; 4 FMVZ-Unesp – Botucatu, Brazil The ovarian cysts was a of high occurrence pathology and for causes significant alterations in the reproductive performance of the animals. Its treatment must be effective, in the direction to minimize the reproductive losses. The result of the successful treatment would be the regression of the cystic structure and formation of a luteal mass (corpus luteum) and the fast return to the cyclical luteal ovarian activity and manifestation of regular estrous cycles. Braun et al. (2000) shows considerable occurrence of cystic structures with partially luteinized wall in dairy cows, and cite the possibility of beneficial effect in the application of prostaglandins associated to the analogous ones of the GnRH for treatment. The objective of this study was to evaluate and to compare the efficiency of the Fertirelin Acetate in association or not with the Cloprostenol for treatment of ovarian cysts in dairy cows. 133 holstein cows between 30 and 90 days postpartum had been used in four Farms. The cyst diagnosis was made by ultrasonography (Esaote-Falco), considering as cyst a anecoic structure above of 20mm beyond the absence of luteal mass in the two ovaries. The animals had been randomized into two groups that had received the following treatments, by IM way in only dose: G1 (n=51): 0,1mg of Fertirelin Acetate (Fertigen Schering Plough- Brazil); G2 (n=49): 0,1mg of Fertirelin Acetate + 0,530mg of Cloprostenol (Ciosin Schering Plough-Brazil) at the same time, and G3 (n=35): Control Group (non treated). All the animals again had been evaluated of 12 the 18 days later. The treatment was considered efficient where in the second ultrasonography evaluation it was detected the absence of the cystic structure and presence of luteal mass. The efficiency of the treatments was evaluated by the χ2 test. The results of the two treatments had been 54.91; 81.63 and 17.14%, for the groups G1; G2 and G3, respectively (P
16 t h International Congress on Animal Reproduction Poster Abstracts 41 analogous one of the GnRH, was beneficial for resolution of this pathology. P040 Bull and sire effect for the pregnancy rate and embryonic loss in dairy cow Gabor, G 1 *, Balogh, OG 1 , Abonyi-Toth, Zs 2 , Toth, F 1 and Sasser, RG 3,4 1Department of Cattle Breeding Research Institute for Animal Breeding, H- 2053 Herceghalom, Hungary, 2 SzIE-AOTK, H-1074 Budapest, Hungary, 3Department of Animal and Veterinary Science, University of Idaho and 4BioTracking LLC, 105 E. 2nd, Moscow, ID, USA Introduction The main limiting factors of reproductive performance in dairies are pregnancy rate (PR) (influenced by embryonic loss (EL)) and number of services per conception (NSC). Between 1st January and 31st October 2007 our laboratory checked early pregnancies of approximately 11200 AI’s. That required collecting data from cows of 13 dairies. PR and NSC seemed to be affected by the AI sire is why data were collected on the AI sires on the farm too. We also recorded ovarian treatments of cows before AI, pregnancy rates and embryonic losses in cows. Our main goal was to check sire and bull effect for PR and EL. Based on the above mentioned data statistical analyses were carried out to find out interactions between PR and EL with other factors (farm, ovarian treatment, age, number of AI). Materials and methods Blood samples were collected once a week, 30-36 days post insemination (PI), and sent to the laboratory by overnight mail. Data about the cow and AI were sent via email. BioPRYN ® ELISA test (BioTracking, Moscow, ID, US) was run for detection of early pregnancy. All open cows and pregnant cows which had low optical density values (close to the cutoff) during the BioPryn test were assayed for serum P4 concentration. P4 was checked by an ELISA test (QuantiCheck, Veterinorg, Budapest). Re-check of pregnancy status was done 60-90 days PI by rectal palpation. Differences between the early and 60-90 days pregnancy detection were called late loss. Pearson's Chi-squared test and logistic regression was used to find the association among the data. Data from AI bulls (AIB) and the bull’s sires (BS) which had more than 100 AI’s, and fathers of cows (FC) whose daughters had more than 100 AI’s were used for statistical analysis. Results and discussion Significant correlation (P
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Reproduction in Domestic Animals - Facultad de Ciencias Veterinarias

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